CYP7B1 (NM_004820) Human Untagged Clone

CAT#: SC316629

CYP7B1 (untagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP7B1
• Synonyms: CBAS3; CP7B; SPG5A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC316629 sequence for NM_004820 edited (data generated by NextGen Sequencing)
  ATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTGGAGCGGTTGGGCCTCCCG
  GGCCTGGCCCTCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGCTTGCTTGTCCGGCGCACC
  AGGAGACCCGGTGAGCCTCCATTGATAAAAGGTTGGCTTCCTTATCTTGGAGTGGTCCTG
  AACTTACGAAAAGACCCCTTAAGGTTCATGAAAACACTTCAAAAGCAACATGGTGACACT
  TTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCCTGGACCCCTTCCAGTACCAG
  CTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATTTTCTAATAAATTATTAGAG
  AAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACATGAATGATGAGCTTCACCTC
  TGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTTGGAAAGCATGATGCAGAAT
  CTAAAACAAGTTTTTGAACCCCAGCTGTTAAAAACCACAAGTTGGGACACGGCAGAACTG
  TATCCATTCTGCAGCTCAATAATATTTGAGATCACATTTACAACTATATATGGAAAAGTT
  ATTGTTTGTGACAACAACAAATTTATTAGTGAGCTAAGAGATGATTTTTTAAAATTTGAT
  GACAAGTTTGCATATTTAGTATCCAACATACCCATTGAGCTTCTAGGAAATGTCAAGTCT
  ATTAGAGAGAAAATTATAAAATGCTTCTCATCAGAAAAGTTAGCCAAGATGCAAGGATGG
  TCAGAAGTTTTTCAAAGCAGGCAAGATGTCCTGGAGAAATATTATGTGCACGAGGACCTT
  GAAATAGGAGCACATCATTTAGGCTTTCTCTGGGCCTCTGTGGCAAACACTATTCCAACT
  ATGTTCTGGGCAATGTATTATCTTCTGCGGCACCCAGAAGCTATGGCAGCAGTGCGTGAC
  GAAATTGACCGTTTGCTGCAGTCAACAGGTCAAAAGAAAGGGTCTGGATTTCCCATCCAC
  CTCACCAGAGAACAATTGGACAGCCTAATCTGCCTAGAAAGCAGCATTTTTGAAGCTTTA
  CGACTGTCCTCATATTCAACCACCATTCGTTTTGTTGAGGAGGATTTGACTCTCAGTTCA
  GAGACCGGGGACTACTGTGTGCGAAAGGGAGACTTGGTAGCCATCTTTCCTCCAGTCCTA
  CATGGTGACCCTGAAATCTTTGAAGCTCCAGAGGAGTTTAGATATGATCGTTTTATAGAA
  GATGGTAAGAAGAAAACCACCTTTTTCAAAAGAGGGAAAAAGCTGAAGTGTTACCTAATG
  CCGTTTGGAACTGGAACCAGCAAATGTCCAGGCCGATTTTTTGCACTTATGGAAATAAAA
  CAATTGTTGGTTATACTTTTAACTTATTTTGATTTAGAAATAATTGATGATAAGCCCATA
  GGACTAAACTACAGCCGCTTGTTGTTTGGTATTCAGTATCCAGATTCTGATGTTTTATTT
  AGATACAAAGTGAAATCTTAG
  
  Clone variation with respect to NM_004820.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004820 unedited
  TGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCACGGTGACATTCCTGTC
  TGCGCTGCGGGCAGGCCAGCCTCTCCGGCCCTGGACTAGGGGGCGCCGCCGAGAGAAGCC
  GCCGCAGGCTGCAGAGTCGCCGACCGCGCTGTTCGCAGGCAGACCCCGGGCTGCCCACCG
  CGGCCTAGCGCGCGCCGGATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTG
  GAGCGGTTGGGCCTCCCGGGCCTGGCCCTCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGC
  TTGCTTGTCCGGCGCACCAGGAGACCCGGTGAGCCTCCATTGATAAAAGGTTGGCTTCCT
  TATCTTGGGAGTGGTCCTGAACTTACGAAAAGACCCCTTAAGGTTCATGAAAACACTTCA
  AAAGCAACATGGTGACACTTTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCCT
  GGACCCCTTCCAGTACCAGCTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATT
  TTCTAATAAATTATTAGAGAAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACAT
  GAATGATGAGCTTCACCTCTGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTT
  GGAAAGCATGATGCAAAATCTAAAACAAGTTTTTGAACCCCAGCTGTTAAAAACCACAAG
  TTGGGACACGGCAGAACTGTATCCATTCTGCAGCTCATATATTTNTGAGACACATTTACA
  CTATTTGGGGAAAGGTTTTTGGTTTGTGACACAACAAATTTATTAGTGAGCTAAGAGAAG
  GATTTTTTAAAATTTGAGGACCAGTTTGGCTTATTTAGTATCAACATACCCTTGG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_004820 unedited
  CAAAGTCCAGCCGATTTTTGCACTTAGGAATAAAACAATTGTTGGTTATACTTTTAACTT
  ATTTTGATTTAGAAATAATTGATGATAAGCCCATAGGACTAAACTACAGCCGCTTGTTGT
  TTGGTATTCAGTATCCAGATTCTGATGTTTTATTTAGCTACAAAGTGAAATCTTAGAGAA
  GCTAAAAGGAAAGAAAATAAATCTATCAAAATTACCCTAAACATCCTAAGCTCATCTATT
  TCTTTTTAATTTCTGCAAATGTAATTGATTTATTTGTTTAAAAAGCGCTAATTTCTATTT
  GATCTGATATCAGTCCAGTTTGTCCTTAGTCACAAAGCCCATCATAATATAAAACAGGAT
  GGTGGCAGGAAAATGGACATCAAAATCAACTTAAGGGTAGGCTCAAAACAGGGTTGTTGT
  TGTTTTTTTAGTTTCTCCTTGTTGTGATTTTCACCTGTTATAATAAATGTACCTTCACAC
  CAATAGATTGGGCACTGTGGAATTTAAATCACTCAAATTGTTCTCTAATATGTAAAATTA
  CACTTTGAACTACTAGAAAATTGTGCTGGAAATGGACACAGTCTAACAAATTCCAAATTC
  TCACAGAAGAAGGAAATTGAATTTCCATGAGTTACACTGGATGAAAATGTTCCCTTCAAG
  TATATATTAATAATACCTATATTTCCAGGGTATGTGTGCATTAAATGAGTNTCACAGTAG
  ATTGAATGCTTCAACTTCACTTNCATATTAAATATGCATAAATGNTNCTTAATACTTTGN
  ATACTATGGCTCATAGAACAAATTNNCTGNNTACATAGACTTNCATGTTCAGNTAGATAC
  TGATAGATTAATGTTAGAACACAGAACTATATTATCAGAAATGCCTANATANAATAAGNN
  TATATGGGAGCAATGTGATAATACGCATGATATTAATTGNCTTAATAACNTAAGGTTGCA
  AATTGCATAACTTTCTAAGCTATTATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004820
• ORF Size: 1521 bp
• Insert Size: 4600
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_004820.2, NP_004811.1
• RefSeq Size: 2230
• RefSeq ORF: 1521
• Locus ID: 9420
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.