AMPK gamma 3 (PRKAG3) (NM_017431) Human Tagged ORF Clone

CAT#: RC222787

PRKAG3 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, gamma 3 non-catalytic subunit (PRKAG3)

Product Images

Western blot validation of overexpression lysate (Cat# LY413786) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222787 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKAG3
• Synonyms: AMPKG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222787 representing NM_017431
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGGCTGGAGCACGCACTGCGCAGGACCCCTTCCTGGAGCAGCCTTGGGGGTTCTGAGCATC
  AAGAGATGAGCTTCCTAGAGCAAGAAAACAGCAGCTCATGGCCATCACCAGCTGTGACCAGCAGCTCAGA
  AAGAATCCGTGGGAAACGGAGGGCCAAAGCCTTGAGATGGACAAGGCAGAAGTCGGTGGAGGAAGGGGAG
  CCACCAGGTCAGGGGGAAGGTCCCCGGTCCAGGCCAGCTGCTGAGTCCACCGGGCTGGAGGCCACATTCC
  CCAAGACCACACCCTTGGCTCAAGCTGATCCTGCCGGGGTGGGCACTCCACCAACAGGGTGGGACTGCCT
  CCCCTCTGACTGTACAGCCTCAGCTGCAGGCTCCAGCACAGATGATGTGGAGCTGGCCACGGAGTTCCCA
  GCCACAGAGGCCTGGGAGTGTGAGCTAGAAGGCCTGCTGGAAGAGAGGCCTGCCCTGTGCCTGTCCCCGC
  AGGCCCCATTTCCCAAGCTGGGCTGGGATGACGAACTGCGGAAACCCGGCGCCCAGATCTACATGCGCTT
  CATGCAGGAGCACACCTGCTACGATGCCATGGCAACTAGCTCCAAGCTAGTCATCTTCGACACCATGCTG
  GAGATCAAGAAGGCCTTCTTTGCTCTGGTGGCCAACGGTGTGCGGGCAGCCCCTCTATGGGACAGCAAGA
  AGCAGAGCTTTGTGGGGATGCTGACCATCACTGACTTCATCCTGGTGCTGCATCGCTACTACAGGTCCCC
  CCTGGTCCAGATCTATGAGATTGAACAACATAAGATTGAGACCTGGAGGGAGATCTACCTGCAAGGCTGC
  TTCAAGCCTCTGGTCTCCATCTCTCCTAATGATAGCCTGTTTGAAGCTGTCTACACCCTCATCAAGAACC
  GGATCCATCGCCTGCCTGTTCTTGACCCGGTGTCAGGCAACGTACTCCACATCCTCACACACAAACGCCT
  GCTCAAGTTCCTGCACATCTTTGGTTCCCTGCTGCCCCGGCCCTCCTTCCTCTACCGCACTATCCAAGAT
  TTGGGCATCGGCACATTCCGAGACTTGGCTGTGGTGCTGGAGACAGCACCCATCCTGACTGCACTGGACA
  TCTTTGTGGACCGGCGTGTGTCTGCACTGCCTGTGGTCAACGAATGTGGTCAGGTCGTGGGCCTCTATTC
  CCGCTTTGATGTGATTCACCTGGCTGCCCAGCAAACCTACAACCACCTGGACATGAGTGTGGGAGAAGCC
  CTGAGGCAGAGGACACTATGTCTGGAGGGAGTCCTTTCCTGCCAGCCCCACGAGAGCTTGGGGGAAGTGA
  TCGACAGGATTGCTCGGGAGCAGGTACACAGGCTGGTGCTAGTGGACGAGACCCAGCATCTCTTGGGCGT
  GGTCTCCCTCTCCGACATCCTTCAGGCACTGGTGCTCAGCCCTGCTGGCATCGATGCCCTCGGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222787 representing NM_017431
  Red=Cloning site Green=Tags(s)
  
  MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGE
  PPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFP
  ATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTML
  EIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGC
  FKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQD
  LGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEA
  LRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017431
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017431.2, NP_059127.2
• RefSeq Size: 2299 bp
• RefSeq ORF: 1470 bp
• Locus ID: 53632
• Domains: CBS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• MW: 54.1 kDa
• Gene Summary: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]