ALDH1A2 (NM_003888) Human Tagged ORF Clone

CAT#: RC223250

ALDH1A2 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418372) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223250 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ALDH1A2 protein (Cat# TP323250). The protein was produced from HEK293T cells transfected with ALDH1A2 cDNA clone (Cat# RC223250) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH1A2
• Synonyms: RALDH(II); RALDH2; RALDH2-T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223250 representing NM_003888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCCAGCAAGATAGAGATGCCCGGCGAGGTGAAGGCCGACCCCGCCGCCCTCATGGCGTCGCTGC
  ACCTCCTGCCGTCGCCCACGCCCAATCTCGAAATTAAGTACACCAAGATCTTTATAAACAACGAGTGGCA
  GAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTATAATCCAGCCACAGGAGAACAGGTGTGTGAAGTTCAA
  GAAGCAGACAAGGCAGATATAGACAAAGCAGTGCAGGCAGCCCGCCTGGCTTTCTCTCTTGGTTCAGTGT
  GGAGAAGGATGGATGCTTCAGAAAGGGGACGTCTGTTGGATAAGCTTGCAGACTTGGTGGAACGGGACAG
  GGCAGTTCTTGCAACCATGGAATCCCTAAATGGTGGCAAACCATTCCTGCAAGCTTTTTATGTGGATTTG
  CAGGGCGTCATCAAAACCTTTCGATATTACGCAGGCTGGGCTGATAAAATTCATGGGATGACCATTCCTG
  TAGATGGAGACTATTTTACCTTTACAAGACATGAACCCATTGGAGTGTGTGGACAGATCATCCCATGGAA
  CTTCCCCCTGCTGATGTTTGCCTGGAAAATAGCTCCAGCTTTGTGCTGTGGCAATACAGTAGTTATTAAG
  CCAGCAGAGCAAACACCACTCAGTGCACTCTACATGGGAGCCCTCATCAAGGAGGCTGGCTTTCCTCCCG
  GGGTCATCAATATTTTGCCAGGATATGGGCCAACGGCTGGGGCAGCAATAGCTTCTCACATTGGCATAGA
  CAAGATTGCATTCACAGGGTCTACTGAGGTTGGAAAGCTTATCCAAGAAGCAGCTGGAAGAAGTAATTTG
  AAGAGAGTAACTCTGGAACTTGGAGGCAAAAGTCCTAATATTATTTTTGCTGATGCTGACTTGGACTATG
  CTGTGGAGCAGGCCCACCAGGGTGTGTTCTTCAATCAAGGTCAGTGCTGCACTGCAGGCTCTCGCATCTT
  CGTGGAGGAGTCCATCTATGAGGAGTTTGTGAGAAGAAGCGTGGAGCGGGCCAAGAGGCGCGTAGTGGGG
  AGTCCCTTTGACCCCACCACTGAGCAGGGTCCCCAGATTGATAAGAAACAGTACAACAAGATCTTGGAAC
  TCATCCAGAGTGGTGTGGCTGAGGGCGCCAAGCTGGAATGTGGAGGCAAAGGACTGGGCCGAAAGGGGTT
  TTTCATTGAGCCCACAGTGTTTTCCAACGTCACTGATGATATGCGGATTGCCAAGGAGGAGATCTTTGGC
  CCTGTTCAGGAAATTTTGAGATTTAAGACGATGGATGAAGTTATCGAAAGAGCCAATAACTCAGACTTTG
  GACTCGTAGCAGCTGTCTTTACTAATGACATCAACAAGGCCCTCACAGTGTCTTCTGCAATGCAAGCTGG
  GACTGTTTGGATCAATTGTTACAATGCCTTAAATGCCCAGAGCCCCTTTGGGGGATTCAAGATGTCTGGA
  AATGGGAGAGAAATGGGAGAATTTGGCTTGCGGGAGTACTCAGAAGTTAAGACGGTGACAGTAAAGATCC
  CCCAGAAGAACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223250 representing NM_003888
  Red=Cloning site Green=Tags(s)
  
  MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQ
  EADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDL
  QGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIK
  PAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNL
  KRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVG
  SPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG
  PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSG
  NGREMGEFGLREYSEVKTVTVKIPQKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003888
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003888.1, NM_003888.2, NM_003888.3, NP_003879.2
• RefSeq Size: 3398
• RefSeq ORF: 1557
• Locus ID: 8854
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism
• MW: 56.5 kDa
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011]