GPLD1 (NM_177483) Human Tagged ORF Clone
CAT#: RC223455
GPLD1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2
"NM_177483" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GPLD1 |
Synonyms | GPIPLD; GPIPLDM; MGC22590; PIGPLD; PIGPLD1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223455 representing NM_177483
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCTTTCAGGTTGTGGCCTGGCCTGCTGATCATGTTGGGTTCTCTCTGCCATAGAGGTTCACCGT GTGGCCTTTCAACACACGTAGAAATAGGACACAGAGCTCTGGAGTTTCTTCAGCTTCACAATGGGCGTGT TAACTACAGAGAGCTGTTACTAGAACACCAGGATGCGTATCAGGCTGGAATCGTGTTTCCTGATTGTTTT TACCCTAGCATCTGCAAAGGAGGAAAATTCCATGATGTGTCTGAGAGCACTCACTGGACTCCGTTTCTTA ATGCAAGCGTTCATTATATCCGAGAGAACTATCCCCTTCCCTGGGAGAAGGACACAGAGAAACTGGTAGC TTTCTTGTTTGGAATTACTTCTCACATGGCGGCAGATGTCAGCTGGCATAGTCTGGGCCTTGAACAAGGA TTCCTTAGGACCATGGGAGCTATTGATTTTCACGGCTCCTATTCAGAGGCTCATTCGGCTGGTGATTTTG GTACTGTTTATTTACATTTGCTAAATTTTCTCGTGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223455 representing NM_177483
Red=Cloning site Green=Tags(s) MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCF YPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQG FLRTMGAIDFHGSYSEAHSAGDFGTVYLHLLNFLVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177483 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177483.1, NP_803436.1 |
RefSeq Size | 1096 bp |
RefSeq ORF | 530 bp |
Locus ID | 2822 |
Cytogenetics | 6p22.3 |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis |
MW | 17.3 kDa |
Gene Summary | 'Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307100 | GPLD1 (untagged)-Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2 |
USD 420.00 |
|
RG223455 | GPLD1 (GFP-tagged) - Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2 |
USD 460.00 |
|
RC223455L1 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC223455L2 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC223455L3 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC223455L4 | Lenti ORF clone of Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 2, mGFP tagged |
USD 620.00 |
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