PAICS (NM_001079525) Human Tagged ORF Clone

CAT#: RC223925

PAICS (Myc-DDK-tagged)-Human phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY421515) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223925 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAICS
• Synonyms: ADE2; ADE2H1; AIRC; PAIS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223925 representing NM_001079525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACAGCTGAGGTACTGAACATTGGTAAAAAATTATATGAGGGTAAAACAAAAGAAGTCTACGAAT
  TGTTAGACAGTCCAGGAAAAGTCCTCCTGCAGTCCAAGGACCAGATTACAGCAGGAAATGCAGCTAGAAA
  AAACCACCTGGAAGGAAAAGCTGCAATCTCAAATAAAATCACCAGTTGTATTTTTCAGTTATTACAGGAA
  GCAGTTACCTCATATAAGTCAAATCGTATTAAAACTGCCTTCACCAGAAAATGTGGGGAGACAGCTTTCA
  TTGCACCGCAGTGTGAAATGATTCCAATTGAATGGGTTTGCAGAAGAATAGCAACTGGTTCTTTTCTCAA
  AAGAAATCCTGGTGTCAAGGAAGGATATAAGTTTTACCCACCTAAAGTGGAGTTGTTTTTCAAGGATGAT
  GCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAAATTTTGCTTTGCTGGACTTCTTA
  TAGGCCAGACTGAAGTGGATATCATGAGTCATGCTACACAGGCTATATTTGAAATACTGGAGAAATCCTG
  GTTGCCCCAGAATTGTACACTGGTTGATATGAAGATTGAATTTGGTGTTGATGTAACCACCAAAGAAATT
  GTTCTTGCTGATGTTATTGACAATGATTCCTGGAGACTCTGGCCATCAGGAGATCGAAGCCAACAGAAAG
  ACAAACAGTCTTATCGGGACCTCAAAGAAGTAACTCCTGAAGGGCTCCAAATGGTAAAGAAAAACTTTGA
  GTGGGTTGCAGAGAGAGTAGAGTTGCTTTTGAAATCAGAAAGTCAGTGCAGGGTTGTAGTGTTGATGGGC
  TCTACTTCTGATCTTGGTCACTGTGAAAAAATCAAGAAGGCCTGTGGAAATTTTGGCATTCCATGTGAAC
  TTCGAGTAACATCTGCGCATAAAGGACCAGATGAAACTCTGAGGATTAAAGCTGAGTATGAAGGGGATGG
  CATTCCTACTGTATTTGTGGCAGTGGCAGGCAGAAGTAATGGTTTGGGACCAGTGATGTCTGGGAACACT
  GCATATCCAGTTATCAGCTGTCCTCCCCTCACACCAGACTGGGGAGTTCAGGATGTGTGGTCTTCTCTTC
  GACTACCCAGTGGTCTTGGCTGTTCAACCGTACTTTCTCCAGAAGGATCAGCTCAATTTGCTGCTCAGAT
  ATTTGGGTTAAGCAACCATTTGGTATGGAGCAAACTGCGAGCAAGCATTTTGAACACATGGATTTCCTTG
  AAGCAGGCTGACAAGAAAATCAGAGAATGTAATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223925 representing NM_001079525
  Red=Cloning site Green=Tags(s)
  
  MATAEVLNIGKKLYEGKTKEVYELLDSPGKVLLQSKDQITAGNAARKNHLEGKAAISNKITSCIFQLLQE
  AVTSYKSNRIKTAFTRKCGETAFIAPQCEMIPIEWVCRRIATGSFLKRNPGVKEGYKFYPPKVELFFKDD
  ANNDPQWSEEQLIAAKFCFAGLLIGQTEVDIMSHATQAIFEILEKSWLPQNCTLVDMKIEFGVDVTTKEI
  VLADVIDNDSWRLWPSGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVAERVELLLKSESQCRVVVLMG
  STSDLGHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVAVAGRSNGLGPVMSGNT
  AYPVISCPPLTPDWGVQDVWSSLRLPSGLGCSTVLSPEGSAQFAAQIFGLSNHLVWSKLRASILNTWISL
  KQADKKIRECNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079525
• ORF Size: 1296 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001079525.1, NP_001072993.1
• RefSeq Size: 3350
• RefSeq ORF: 1299
• Locus ID: 10606
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 47.8 kDa
• Gene Summary: This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]