PHEMX (TSPAN32) (NM_139022) Human Tagged ORF Clone

CAT#: RC224210

TSPAN32 (Myc-DDK-tagged)-Human tetraspanin 32 (TSPAN32)

Product Images

Western blot validation of overexpression lysate (Cat# LY408323) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224210 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TSPAN32
• Synonyms: ART1; PHEMX; PHMX; TSSC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224210 representing NM_139022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCTTGGAGTCGAGTCAGGGTTGCCAAATGCCAGATGCTGGTCACCTGCTTCTTTATCTTGCTGC
  TGGGCCTCTCTGTGGCCACCATGGTGACTCTTACCTACTTCGGGGCCCACTTTGCTGTCATCCGCCGAGC
  GTCCCTGGAGAAGAACCCGTACCAGGCTGTGCACCAATGGGCCTTCTCTGCGGGGTTGAGCCTGGTGGGC
  CTCCTGACTCTGGGAGCCGTGCTGAGCGCTGCAGCCACCGTGAGGGAGGCCCAGGGCCTCATGGCAGGGG
  GCTTCCTGTGCTTCTCCCTGGCGTTCTGCGCACAGGTGCAGGTGGTGTTCTGGAGACTCCACAGCCCCAC
  CCAGGTGGAGGACGCCATGCTGGACACCTACGACCTGGTATATGAGCAGGCGATGAAAGGTACGTCCCAC
  GTCCGGCGGCAGGAGCTGGCGGCCATCCAGGACGTGTTTCTGTGCTGTGGGAAGAAGTCTCCTTTCAGCC
  GTCTGGGGAGCACAGAGGCTGACCTGTGTCAGGGAGAGGAGGCGGCGAGAGAGGACTGCCTTCAGGGCAT
  CCGGAGCTTCCTGAGGACACACCAGCAGGTCGCCTCCAGCCTGACCAGCATCGGCCTGGCCCTCACGGTG
  TCCGCCTTGCTCTTCAGCTCCTTCCTGTGGTTTGCCATCCGCTGTGGCTGCAGCTTGGACCGCAAGGGCA
  AATACACCCTGACCCCACGAGCATGTGGCCGCCAGCCCCAGGAGCCCAGCCTCTTGAGATGCTCCCAGGG
  TGGACCCACACATTGTCTCCACTCCGAAGCAGTTGCTATTGGTCCAAGAGGATGCTCGGGTAGTCTTCGG
  TGGCTGCAGGAGAGCGATGCTGCGCCTCTGCCCCTCTCCTGCCACCTGGCTGCCCACAGAGCTCTCCAGG
  GCAGAAGTCGCGGTGGGCTCAGTGGGTGCCCTGAGCGGGGTCTCTCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224210 representing NM_139022
  Red=Cloning site Green=Tags(s)
  
  MGPWSRVRVAKCQMLVTCFFILLLGLSVATMVTLTYFGAHFAVIRRASLEKNPYQAVHQWAFSAGLSLVG
  LLTLGAVLSAAATVREAQGLMAGGFLCFSLAFCAQVQVVFWRLHSPTQVEDAMLDTYDLVYEQAMKGTSH
  VRRQELAAIQDVFLCCGKKSPFSRLGSTEADLCQGEEAAREDCLQGIRSFLRTHQQVASSLTSIGLALTV
  SALLFSSFLWFAIRCGCSLDRKGKYTLTPRACGRQPQEPSLLRCSQGGPTHCLHSEAVAIGPRGCSGSLR
  WLQESDAAPLPLSCHLAAHRALQGRSRGGLSGCPERGLSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139022
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139022.1, NM_139022.2, NP_620591.3
• RefSeq Size: 1376
• RefSeq ORF: 963
• Locus ID: 10077
• Protein Families: Transmembrane
• MW: 34.5 kDa
• Gene Summary: This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]