MFI2 (MELTF) (NM_005929) Human Tagged ORF Clone

CAT#: RC224600

MFI2 (Myc-DDK-tagged)-Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401797) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224600 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MELTF protein (Cat# TP324600). The protein was produced from HEK293T cells transfected with MELTF cDNA clone (Cat# RC224600) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MELTF
• Synonyms: CD228; MAP97; MFI2; MTf; MTF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224600 representing NM_005929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGTCCGAGCGGGGCTCTGTGGCTGCTCCTGGCTCTGCGCACCGTGCTCGGTGGCATGGAGGTGC
  GGTGGTGCGCCACCTCGGACCCAGAGCAGCACAAGTGCGGCAACATGAGCGAGGCCTTCCGGGAAGCGGG
  CATCCAGCCCTCCCTCCTCTGCGTCCGGGGCACCTCCGCCGACCACTGCGTCCAGCTCATCGCGGCCCAG
  GAGGCTGACGCCATCACTCTGGATGGAGGAGCCATCTATGAGGCGGGAAAGGAGCACGGCCTGAAGCCGG
  TGGTGGGCGAAGTGTACGATCAAGAGGTCGGTACCTCCTATTACGCCGTGGCTGTGGTCAGGAGGAGCTC
  CCATGTGACCATTGACACCCTGAAAGGCGTGAAGTCCTGCCACACGGGCATCAATCGCACAGTGGGCTGG
  AACGTGCCCGTGGGCTACCTGGTGGAGAGCGGCCGCCTCTCGGTGATGGGCTGCGATGTACTCAAAGCTG
  TCAGCGACTATTTTGGGGGCAGCTGCGTCCCGGGGGCAGGAGAGACCAGTTACTCTGAGTCCCTCTGTCG
  CCTCTGCAGGGGTGACAGCTCTGGGGAAGGGGTGTGTGACAAGAGCCCCCTGGAGAGATACTACGACTAC
  AGCGGGGCCTTCCGGTGCCTGGCGGAAGGGGCAGGGGACGTGGCTTTTGTGAAGCACAGCACGGTACTGG
  AGAACACGGATGGGAAGACGCTTCCCTCCTGGGGCCAGGCCCTGCTGTCACAGGACTTCGAGCTGCTGTG
  CCGGGATGGTAGCCGGGCCGATGTCACCGAGTGGAGGCAGTGCCATCTGGCCCGGGTGCCTGCTCACGCC
  GTGGTGGTCCGGGCCGACACAGATGGGGGCCTCATCTTCCGGCTGCTCAACGAAGGCCAGCGTCTGTTCA
  GCCACGAGGGCAGCAGCTTCCAGATGTTCAGCTCTGAGGCCTATGGCCAGAAGGATCTACTCTTCAAAGA
  CTCTACCTCGGAGCTTGTGCCCATCGCCACACAGACCTATGAGGCGTGGCTGGGCCATGAGTACCTGCAC
  GCCATGAAGGGTCTGCTCTGTGACCCCAACCGGCTGCCCCCCTACCTGCGCTGGTGTGTGCTCTCCACTC
  CCGAGATCCAGAAGTGTGGAGACATGGCCGTGGCCTTCCGCCGGCAGCGGCTCAAGCCAGAGATCCAGTG
  CGTGTCAGCCAAGTCCCCCCAACACTGCATGGAGCGGATCCAGGCTGAGCAGGTCGACGCTGTGACCCTG
  AGTGGCGAGGACATTTACACGGCGGGGAAGACGTACGGCCTGGTTCCCGCAGCCGGGGAGCACTATGCCC
  CGGAAGACAGCAGCAACTCGTACTACGTGGTGGCCGTGGTGAGACGGGACAGCTCCCACGCCTTCACCTT
  GGATGAGCTTCGGGGCAAGCGCTCCTGCCACGCCGGTTTCGGCAGCCCTGCAGGCTGGGATGTCCCCGTG
  GGTGCCCTTATTCAGAGAGGCTTCATCCGGCCCAAGGACTGTGACGTCCTCACAGCAGTGAGCGAGTTCT
  TCAATGCCAGCTGCGTGCCCGTGAACAACCCCAAGAACTACCCCTCCTCGCTGTGTGCACTGTGCGTGGG
  GGACGAGCAGGGCCGCAACAAGTGTGTGGGCAACAGCCAGGAGCGGTATTACGGCTACCGCGGCGCCTTC
  AGGTGCCTGGTGGAGAATGCGGGTGACGTTGCCTTCGTCAGGCACACAACCGTCTTTGACAACACAAACG
  GCCACAATTCCGAGCCCTGGGCTGCTGAGCTCAGGTCAGAGGACTATGAACTGCTGTGCCCCAACGGGGC
  CCGAGCCGAGGTGTCCCAGTTTGCAGCCTGCAACCTGGCACAGATACCACCCCACGCCGTGATGGTCCGG
  CCCGACACCAACATCTTCACCGTGTATGGACTGCTGGACAAGGCCCAGGACCTGTTTGGAGACGACCACA
  ATAAGAACGGGTTCAAAATGTTCGACTCCTCCAACTATCATGGCCAAGACCTGCTTTTCAAGGATGCCAC
  CGTCCGGGCGGTGCCTGTCGGAGAGAAAACCACCTACCGCGGCTGGCTGGGGCTGGACTACGTGGCGGCG
  CTGGAAGGGATGTCGTCTCAGCAGTGCTCGGGCGCAGCGGCCCCGGCGCCCGGGGCGCCCCTGCTCCCGC
  TGCTGCTGCCCGCCCTCGCCGCCCGCCTGCTCCCGCCCGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224600 representing NM_005929
  Red=Cloning site Green=Tags(s)
  
  MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQ
  EADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGW
  NVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDY
  SGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHA
  VVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLH
  AMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTL
  SGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPV
  GALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAF
  RCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVR
  PDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAA
  LEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005929
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005929.1, NM_005929.2, NM_005929.3, NM_005929.4, NM_005929.5, NP_005920.1, NP_005920.2
• RefSeq Size: 2377 bp
• RefSeq ORF: 2217 bp
• Locus ID: 4241
• Cytogenetics: 3q29
• Protein Families: Druggable Genome
• MW: 80.21 kDa
• Gene Summary: 'The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]'