MFI2 (MELTF) (NM_005929) Human Untagged Clone

CAT#: SC303705

MFI2 (untagged)-Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MELTF
• Synonyms: CD228; MAP97; MFI2; MTf; MTF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005929, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGGGTCCGAGCGGGGCTCTGTGGCTGCTCCTGGCTCTGCGCACCGTGCTCGGTGGCATGGAGGTGC
  GGTGGTGCGCCACCTCGGACCCAGAGCAGCACAAGTGCGGCAACATGAGCGAGGCCTTCCGGGAAGCGGG
  CATCCAGCCCTCCCTCCTCTGCGTCCGGGGCACCTCCGCCGACCACTGCGTCCAGCTCATCGCGGCCCAG
  GAGGCTGACGCCATCACTCTGGATGGAGGAGCCATCTATGAGGCGGGAAAGGAGCACGGCCTGAAGCCGG
  TGGTGGGCGAAGTGTACGATCAAGAGGTCGGTACCTCCTATTACGCCGTGGCTGTGGTCAGGAGGAGCTC
  CCATGTGACCATTGACACCCTGAAAGGCGTGAAGTCCTGCCACACGGGCATCAATCGCACAGTGGGCTGG
  AACGTGCCCGTGGGCTACCTGGTGGAGAGCGGCCGCCTCTCGGTGATGGGCTGCGATGTACTCAAAGCTG
  TCAGCGACTATTTTGGGGGCAGCTGCGTCCCGGGGGCAGGAGAGACCAGTTACTCTGAGTCCCTCTGTCG
  CCTCTGCAGGGGTGACAGCTCTGGGGAAGGGGTGTGTGACAAGAGCCCCCTGGAGAGATACTACGACTAC
  AGCGGGGCCTTCCGGTGCCTGGCGGAAGGGGCAGGGGACGTGGCTTTTGTGAAGCACAGCACGGTACTGG
  AGAACACGGATGGGAAGACGCTTCCCTCCTGGGGCCAGGCCCTGCTGTCACAGGACTTCGAGCTGCTGTG
  CCGGGATGGTAGCCGGGCCGATGTCACCGAGTGGAGGCAGTGCCATCTGGCCCGGGTGCCTGCTCACGCC
  GTGGTGGTCCGGGCCGACACAGATGGGGGCCTCATCTTCCGGCTGCTCAACGAAGGCCAGCGTCTGTTCA
  GCCACGAGGGCAGCAGCTTCCAGATGTTCAGCTCTGAGGCCTATGGCCAGAAGGATCTACTCTTCAAAGA
  CTCTACCTCGGAGCTTGTGCCCATCGCCACACAGACCTATGAGGCGTGGCTGGGCCATGAGTACCTGCAC
  GCCATGAAGGGTCTGCTCTGTGACCCCAACCGGCTGCCCCCCTACCTGCGCTGGTGTGTGCTCTCCACTC
  CCGAGATCCAGAAGTGTGGAGACATGGCCGTGGCCTTCCGCCGGCAGCGGCTCAAGCCAGAGATCCAGTG
  CGTGTCAGCCAAGTCCCCCCAACACTGCATGGAGCGGATCCAGGCTGAGCAGGTCGACGCTGTGACCCTG
  AGTGGCGAGGACATTTACACGGCGGGGAAGACGTACGGCCTGGTTCCCGCAGCCGGGGAGCACTATGCCC
  CGGAAGACAGCAGCAACTCGTACTACGTGGTGGCCGTGGTGAGACGGGACAGCTCCCACGCCTTCACCTT
  GGATGAGCTTCGGGGCAAGCGCTCCTGCCACGCCGGTTTCGGCAGCCCTGCAGGCTGGGATGTCCCCGTG
  GGTGCCCTTATTCAGAGAGGCTTCATCCGGCCCAAGGACTGTGACGTCCTCACAGCAGTGAGCGAGTTCT
  TCAATGCCAGCTGCGTGCCCGTGAACAACCCCAAGAACTACCCCTCCTCGCTGTGTGCACTGTGCGTGGG
  GGACGAGCAGGGCCGCAACAAGTGTGTGGGCAACAGCCAGGAGCGGTATTACGGCTACCGCGGCGCCTTC
  AGGTGCCTGGTGGAGAATGCGGGTGACGTTGCCTTCGTCAGGCACACAACCGTCTTTGACAACACAAACG
  GCCACAATTCCGAGCCCTGGGCTGCTGAGCTCAGGTCAGAGGACTATGAACTGCTGTGCCCCAACGGGGC
  CCGAGCCGAGGTGTCCCAGTTTGCAGCCTGCAACCTGGCACAGATACCACCCCACGCCGTGATGGTCCGG
  CCCGACACCAACATCTTCACCGTGTATGGACTGCTGGACAAGGCCCAGGACCTGTTTGGAGACGACCACA
  ATAAGAACGGGTTCAAAATGTTCGACTCCTCCAACTATCATGGCCAAGACCTGCTTTTCAAGGATGCCAC
  CGTCCGGGCGGTGCCTGTCGGAGAGAAAACCACCTACCGCGGCTGGCTGGGGCTGGACTACGTGGCGGCG
  CTGGAAGGGATGTCGTCTCAGCAGTGCTCGGGCGCAGCGGCCCCGGCGCCCGGGGCGCCCCTGCTCCCGC
  TGCTGCTGCCCGCCCTCGCCGCCCGCCTGCTCCCGCCCGCCCTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005929
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005929.5, NP_005920.2
• RefSeq Size: 3963 bp
• RefSeq ORF: 2217 bp
• Locus ID: 4241
• Cytogenetics: 3q29
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1), as compared to variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.