DYRK3 (NM_003582) Human Tagged ORF Clone

CAT#: RC224654

DYRK3 (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYRK3
• Synonyms: DYRK5; hYAK3-2; RED; REDK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224654 representing NM_003582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGCACAGCTCGTGGGCCTGGGCGGAAGGATGCGGGGCCGCCTGGGGCCGGGCTCCCGCCCCAGC
  AGCGGAGGTTGGGGGATGGTGTCTATGACACCTTCATGATGATAGATGAAACCAAATGTCCCCCCTGTTC
  AAATGTACTCTGCAATCCTTCTGAACCACCTCCACCCAGAAGACTAAATATGACCACTGAGCAGTTTACA
  GGAGATCATACTCAGCACTTTTTGGATGGAGGTGAGATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCA
  ACAGAAAATCCAATACTATTCAGTCAGATGGCATCAGTGACTCTGAAAAATGCTCTCCTACTGTTTCTCA
  GGGTAAAAGTTCAGATTGCTTGAATACAGTAAAATCCAACAGTTCATCCAAGGCACCCAAAGTGGTGCCT
  CTGACTCCAGAACAAGCCCTGAAGCAATATAAACACCACCTCACTGCCTATGAGAAACTGGAAATAATTA
  ATTATCCAGAAATTTACTTTGTAGGTCCAAATGCCAAGAAAAGACATGGAGTTATTGGTGGTCCCAATAA
  TGGAGGGTATGATGATGCAGATGGGGCCTATATTCATGTACCTCGAGACCATCTAGCTTATCGATATGAG
  GTGCTGAAAATTATTGGCAAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT
  ACGTGGCCCTAAAAATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT
  GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC
  CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTTTATGAGCTGATTAAAAAAAATAAGT
  TTCAGGGTTTTAGCGTCCAGTTGGTACGCAAGTTTGCCCAGTCCATCTTGCAATCTTTGGATGCCCTCCA
  CAAAAATAAGATTATTCACTGCGATCTGAAGCCAGAAAACATTCTCCTGAAACACCACGGGCGCAGTTCA
  ACCAAGGTCATTGACTTTGGGTCCAGCTGTTTCGAGTACCAGAAGCTCTACACATATATCCAGTCTCGGT
  TCTACAGAGCTCCAGAAATCATCTTAGGAAGCCGCTACAGCACACCAATTGACATATGGAGTTTTGGCTG
  CATCCTTGCAGAACTTTTAACAGGACAGCCTCTCTTCCCTGGAGAGGATGAAGGAGACCAGTTGGCCTGC
  ATGATGGAGCTTCTAGGGATGCCACCACCAAAACTTCTGGAGCAATCCAAACGTGCCAAGTACTTTATTA
  ATTCCAAGGGCATACCCCGCTACTGCTCTGTGACTACCCAGGCAGATGGGAGGGTTGTGCTTGTGGGGGG
  TCGCTCACGTAGGGGTAAAAAGCGGGGTCCCCCAGGCAGCAAAGACTGGGGGACAGCACTGAAAGGGTGT
  GATGACTACTTGTTTATAGAGTTCTTGAAAAGGTGTCTTCACTGGGACCCCTCTGCCCGCTTGACCCCAG
  CTCAAGCATTAAGACACCCTTGGATTAGCAAGTCTGTCCCCAGACCTCTCACCACCATAGACAAGGTGTC
  AGGGAAACGGGTAGTTAATCCTGCAAGTGCTTTCCAGGGATTGGGTTCTAAGCTGCCTCCAGTTGTTGGA
  ATAGCCAATAAGCTTAAAGCTAACTTAATGTCAGAAACCAATGGTAGTATACCCCTATGCAGTGTATTGC
  CAAAACTGATTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224654 representing NM_003582
  Red=Cloning site Green=Tags(s)
  
  MGGTARGPGRKDAGPPGAGLPPQQRRLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMTTEQFT
  GDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSSSKAPKVVP
  LTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYE
  VLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTF
  RNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSS
  TKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLAC
  MMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKDWGTALKGC
  DDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTTIDKVSGKRVVNPASAFQGLGSKLPPVVG
  IANKLKANLMSETNGSIPLCSVLPKLIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003582
• ORF Size: 1764 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003582.2, NP_003573.2
• RefSeq Size: 2207
• RefSeq ORF: 1767
• Locus ID: 8444
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• MW: 65.5 kDa
• Gene Summary: This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]