DYRK3 (NM_003582) Human Untagged Clone

CAT#: SC323461

DYRK3 (untagged)-Kinase deficient mutant (K238M) of Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK3
• Synonyms: DYRK5; hYAK3-2; RED; REDK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003582, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGGCACAGCTCGTGGGCCTGGGCGGAAGGATGCGGGGCCGCCTGGGGCCGGGCTCCCGCCCCAGC
  AGCGGAGGTTGGGGGATGGTGTCTATGACACCTTCATGATGATAGATGAAACCAAATGTCCCCCCTGTTC
  AAATGTACTCTGCAATCCTTCTGAACCACCTCCACCCAGAAGACTAAATATGACCACTGAGCAGTTTACA
  GGAGATCATACTCAGCACTTTTTGGATGGAGGTGAGATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCA
  ACAGAAAATCCAATACTATTCAGTCAGATGGCATCAGTGACTCTGAAAAATGCTCTCCTACTGTTTCTCA
  GGGTAAAAGTTCAGATTGCTTGAATACAGTAAAATCCAACAGTTCATCCAAGGCACCCAAAGTGGTGCCT
  CTGACTCCAGAACAAGCCCTGAAGCAATATAAACACCACCTCACTGCCTATGAGAAACTGGAAATAATTA
  ATTATCCAGAAATTTACTTTGTAGGTCCAAATGCCAAGAAAAGACATGGAGTTATTGGTGGTCCCAATAA
  TGGAGGGTATGATGATGCAGATGGGGCCTATATTCATGTACCTCGAGACCATCTAGCTTATCGATATGAG
  GTGCTGAAAATTATTGGCAAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT
  ACGTGGCCCTAAAAATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT
  GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC
  CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTTTATGAGCTGATTAAAAAAAATAAGT
  TTCAGGGTTTTAGCGTCCAGTTGGTACGCAAGTTTGCCCAGTCCATCTTGCAATCTTTGGATGCCCTCCA
  CAAAAATAAGATTATTCACTGCGATCTGAAGCCAGAAAACATTCTCCTGAAACACCACGGGCGCAGTTCA
  ACCAAGGTCATTGACTTTGGGTCCAGCTGTTTCGAGTACCAGAAGCTCTACACATATATCCAGTCTCGGT
  TCTACAGAGCTCCAGAAATCATCTTAGGAAGCCGCTACAGCACACCAATTGACATATGGAGTTTTGGCTG
  CATCCTTGCAGAACTTTTAACAGGACAGCCTCTCTTCCCTGGAGAGGATGAAGGAGACCAGTTGGCCTGC
  ATGATGGAGCTTCTAGGGATGCCACCACCAAAACTTCTGGAGCAATCCAAACGTGCCAAGTACTTTATTA
  ATTCCAAGGGCATACCCCGCTACTGCTCTGTGACTACCCAGGCAGATGGGAGGGTTGTGCTTGTGGGGGG
  TCGCTCACGTAGGGGTAAAAAGCGGGGTCCCCCAGGCAGCAAAGACTGGGGGACAGCACTGAAAGGGTGT
  GATGACTACTTGTTTATAGAGTTCTTGAAAAGGTGTCTTCACTGGGACCCCTCTGCCCGCTTGACCCCAG
  CTCAAGCATTAAGACACCCTTGGATTAGCAAGTCTGTCCCCAGACCTCTCACCACCATAGACAAGGTGTC
  AGGGAAACGGGTAGTTAATCCTGCAAGTGCTTTCCAGGGATTGGGTTCTAAGCTGCCTCCAGTTGTTGGA
  ATAGCCAATAAGCTTAAAGCTAACTTAATGTCAGAAACCAATGGTAGTATACCCCTATGCAGTGTATTGC
  CAAAACTGATTAGCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003582 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTATGACACCTTCA
  TGATGATAGATGAAACCAAATGTCCCCCCTGTTCAAATGTACTCTGCAATCCTTCTGAACCACCTCCACC
  CAGAAGACTAAATATGACCACTGAGCAGTTTACAGGAGATCATACTCAGCACTTTTTGGATGGAGGTGAG
  ATGAAGGTAGAACAGCTGTTTCAAGAATTTGGCAACAGAAAAATCCAATACTATTTCAGTCAGATGGGCA
  TCAGTGACTCTGAAAAAATGCTCCTCCTACTGTTTCTCAGGGTAAAAAGTTCAGAATTGCTTGAATTACA
  GTAAAATCCAACAGTTTCATCCCAAGGGCACCCCAAGTTGGGTGCCCTCGGAACTCCGGAACAACCCTTG
  GAGCCATTTTAACCACCAACCTCATTGCCAATAGAAAACTGGAATTAATTTATTTATCCAGAAATTTACT
  TTGTGGGTCCAAATGCCAGAAAAGACATGGGGAGTTATTGGGGTTCCAAATAGGGGGGGTTAAATGATGC
  CATGGGGGCCCAATTTCTAGTGACCCGAGACCCACTATGCTTAACGAAATGAAGGTTGCGCTGAAATATT
  TGGCAAGGGGAGATTTGGGCAGGTGGGGCCGGGCTATGATTATCAAACTTTACAGTACGTGGGGCCCATG
  ATAGTGCGGCACTGAGGAGAAGCCTTTATCTCTAGCGCTGAGAGGATCTGCATTTGTAGCACTTATAAGA
  AGAGAACGTGTAGTACGTGTTCACACGCTGCGAGATTACTACGCACACGTTGTCGGCGCGATAGTGCATA
  CATCTAAACGATTAACAATTCGTGTATCACGCTTAGAGCAATGAGCACACATCGCCGACTCGATGGCTTC
  ACGACATAAAGAT
  
  Kinase domain sequence

  >SC323461 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TRRGGKCTGAATTATTGGCAGGGGAGTTTTGGGCAGGTGGCCAGGGTCTATGATCACAAACTTCGACAGT
  ACGTGGCCCTAATGATGGTGCGCAATGAGAAGCGCTTTCATCGTCAAGCAGCTGAGGAGATCCGGATTTT
  GGAGCATCTTAAGAAACAGGATAAAACTGGTAGTATGAACGTTATCCACATGCTGGAAAGTTTCACATTC
  CGGAACCATGTTTGCATGGCCTTTGAATTGCTGAGCATAGACCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_003582
• ORF Size: 1662 bp
• Insert Size: 2050
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_003582.1, NP_003573.1
• RefSeq Size: 2141
• RefSeq ORF: 1662
• Locus ID: 8444
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript, but encodes the longer isoform (a), also known as the RED-L isoform.