ATG9B (NM_173681) Human Tagged ORF Clone

CAT#: RC224817

ATG9B (Myc-DDK-tagged)-Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B)

Product Images

Western blot validation of overexpression lysate (Cat# LY406556) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224817 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG9B
• Synonyms: APG9L2; NOS3AS; SONE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224817 representing NM_173681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGCCGAATGGGCTGGGGGGGGAGAAGAAGGCGGCTGGGGCGGTGGGGAGATCTGGGGCCCGGAT
  CGGTGCCCCTCCTCCCCATGCCACTGCCACCTCCTCCTCCTCCTTCATGCCGGGGACCTGGGGGAGGGAG
  GATCTCCATCTTCTCTCTGTCCCCTGCCCCTCATACAAGAAGCTCCCCCTCCTCATTTTCCCCTCCCACC
  GCAGGGCCCCCTTGCTCAGTGCTACAGGGGACAGGGGCTTCTCAGTCTTGCCACAGTGCTCTCCCTATCC
  CAGCCACCCCCCCAACACAGGCTCAACCTGCAATGACACCTGCCTCTGCATCTCCCTCCTGGGGATCCCA
  CTCCACCCCACCCCTGGCCCCGGCAACCCCCACTCCCTCACAGCAGTGCCCCCAGGACTCTCCTGGGCTG
  CGGGTAGGCCCTTTGATCCCTGAACAGGATTATGAGCGGCTGGAGGACTGTGACCCTGAGGGGTCCCAAG
  ACTCACCCATCCACGGGGAGGAGCAGCAACCCCTGCTTCATGTCCCTGAAGGGCTCCGAGGCTCCTGGCA
  TCACATCCAGAACCTGGACAGTTTCTTCACCAAGATCTACAGCTACCACCAGCGGAATGGCTTTGCCTGC
  ATCTTGCTGGAGGATGTCTTCCAGCTGGGACAATTTATTTTCATTGTCACCTTCACAACCTTCCTCCTTC
  GATGCGTGGATTACAATGTTCTCTTTGCCAACCAACCAAGTAACCATACCAGACCTGGGCCGTTCCACAG
  CAAAGTGACCCTGTCAGATGCCATCCTACCCTCAGCCCAGTGTGCTGAGAGGATCCGCTCCAGCCCGCTG
  CTGGTCCTCCTCCTGGTCCTGGCTGCCGGCTTCTGGCTGGTCCAACTGCTTCGCTCAGTCTGCAACCTCT
  TCAGCTACTGGGACATCCAGGTGTTTTACAGGGAGGCCCTGCACATCCCCCCGGAGGAGCTGAGCTCGGT
  TCCCTGGGCAGAGGTGCAGTCCCGCCTCTTGGCACTGCAGCGGAGCGGGGGCCTGTGCGTGCAGCCGCGG
  CCCCTGACGGAGCTGGACATCCACCACCGCATCCTGCGCTACACCAACTACCAGGTGGCGCTGGCCAACA
  AAGGCCTGCTGCCGGCCCGCTGCCCGCTGCCCTGGGGAGGCAGTGCGGCTTTCCTCAGCCGCGGCCTGGC
  GCTCAATGTCGACCTGCTGCTCTTCCGCGGTCCCTTCTCGCTCTTCCGCGGGGGCTGGGAGCTGCCGCAC
  GCCTACAAGCGCAGCGACCAGCGGGGCGCCCTAGCAGCGCGCTGGGGGCGCACAGTGCTGCTGCTGGCCG
  CCCTGAACCTGGCGCTGAGCCCGCTGGTGCTGGCCTGGCAGGTTCTGCACGTCTTCTATAGCCACGTGGA
  GCTGCTGCGGCGCGAGCCTGGCGCGCTGGGGGCGCGCGGCTGGTCCCGCCTGGCGCGCTTGCAGCTGCGC
  CACTTCAACGAGCTGCCGCACGAGCTGCGCGCGCGCCTGGCCCGCGCCTACCGCCCCGCCGCCGCCTTCC
  TGCGCACCGCTGCGCCCCCCGCGCCCCTGCGCACGCTGCTGGCCCGCCAGCTCGTTTTCTTCGCGGGTGC
  ACTCTTCGCCGCGCTGCTTGTGCTCACCGTCTACGACGAGGACGTGCTAGCCGTGGAGCACGTGCTCACC
  GCCATGACCGCGCTCGGGGTCACCGCCACCGTCGCCAGGTCTTTCATTCCGGAAGAGCAGTGCCAGGGTC
  GTGCGCCGCAGCTCCTGCTGCAGACAGCCCTGGCCCACATGCACTACCTCCCGGAGGAGCCCGGCCCCGG
  CGGCAGGGACCGCGCCTACCGGCAGATGGCGCAGCTGCTGCAGTACCGAGCGGTCTCCCTCCTGGAGGAG
  CTCCTGTCCCCGCTCCTCACCCCGCTGTTTCTGCTTTTCTGGTTCCGCCCTCGTGCCCTGGAGATTATCG
  ACTTTTTTCATCACTTCACTGTGGATGTGGCTGGGGTTGGGGACATCTGTTCCTTTGCCCTTATGGATGT
  GAAGCGCCACGGACACCCTCAGTGGCTCTCGGCGGGACAGACTGAGGCCTCGCTGTCTCAGCGTGCGGAG
  GACGGCAAGACTGAGCTTTCTTTGATGCGGTTCTCCCTGGCGCATCCACTCTGGCGCCCCCCAGGGCACA
  GCTCTAAGTTTCTTGGGCACCTCTGGGGCCGAGTACAACAAGATGCAGCTGCCTGGGGTGCCACCTCGGC
  TCGCGGCCCCTCCACCCCGGGGGTGCTCAGCAACTGCACCTCGCCCCTGCCTGAGGCCTTCCTGGCCAAC
  CTCTTCGTGCACCCTCTCCTGCCTCCGAGAGATCTGAGCCCGACAGCCCCCTGTCCAGCTGCGGCCACAG
  CCAGCCTCCTTGCCTCCATTTCCCGAATTGCCCAGGACCCCAGCTCTGTGTCCCCAGGAGGCACTGGGGG
  CCAGAAGCTGGCCCAGCTCCCAGAACTTGCTTCTGCCGAGATGAGTCTCCATGTCATCTACCTGCACCAG
  CTTCACCAGCAGCAGCAGCAGCAGGAGCCGTGGGGTGAGGCTGCAGCCTCCATCCTGTCCAGGCCCTGCT
  CCAGCCCCTCACAGCCACCCTCGCCTGATGAGGAGAAGCCATCCTGGTCAAGTGACGGCTCCAGTCCTGC
  CTCTAGCCCCAGACAACAGTGGGGAACCCAGAAGGCCCGGAATCTGTTCCCCGGAGGGTTTCAGGTGACC
  ACAGACACCCAGAAGGAGCCTGACCGGGCCTCTTGCACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224817 representing NM_173681
  Red=Cloning site Green=Tags(s)
  
  MVSRMGWGGRRRRLGRWGDLGPGSVPLLPMPLPPPPPPSCRGPGGGRISIFSLSPAPHTRSSPSSFSPPT
  AGPPCSVLQGTGASQSCHSALPIPATPPTQAQPAMTPASASPSWGSHSTPPLAPATPTPSQQCPQDSPGL
  RVGPLIPEQDYERLEDCDPEGSQDSPIHGEEQQPLLHVPEGLRGSWHHIQNLDSFFTKIYSYHQRNGFAC
  ILLEDVFQLGQFIFIVTFTTFLLRCVDYNVLFANQPSNHTRPGPFHSKVTLSDAILPSAQCAERIRSSPL
  LVLLLVLAAGFWLVQLLRSVCNLFSYWDIQVFYREALHIPPEELSSVPWAEVQSRLLALQRSGGLCVQPR
  PLTELDIHHRILRYTNYQVALANKGLLPARCPLPWGGSAAFLSRGLALNVDLLLFRGPFSLFRGGWELPH
  AYKRSDQRGALAARWGRTVLLLAALNLALSPLVLAWQVLHVFYSHVELLRREPGALGARGWSRLARLQLR
  HFNELPHELRARLARAYRPAAAFLRTAAPPAPLRTLLARQLVFFAGALFAALLVLTVYDEDVLAVEHVLT
  AMTALGVTATVARSFIPEEQCQGRAPQLLLQTALAHMHYLPEEPGPGGRDRAYRQMAQLLQYRAVSLLEE
  LLSPLLTPLFLLFWFRPRALEIIDFFHHFTVDVAGVGDICSFALMDVKRHGHPQWLSAGQTEASLSQRAE
  DGKTELSLMRFSLAHPLWRPPGHSSKFLGHLWGRVQQDAAAWGATSARGPSTPGVLSNCTSPLPEAFLAN
  LFVHPLLPPRDLSPTAPCPAAATASLLASISRIAQDPSSVSPGGTGGQKLAQLPELASAEMSLHVIYLHQ
  LHQQQQQQEPWGEAAASILSRPCSSPSQPPSPDEEKPSWSSDGSSPASSPRQQWGTQKARNLFPGGFQVT
  TDTQKEPDRASCTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173681
• ORF Size: 2772 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173681.2, NM_173681.3, NM_173681.5, NP_775952.2, NP_775952.4
• RefSeq Size: 4800
• RefSeq ORF: 2775
• Locus ID: 285973
• Protein Families: Transmembrane
• MW: 100.8 kDa
• Gene Summary: This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]