Phosphatidic acid phosphatase type 2B (PLPP3) (NM_177414) Human Tagged ORF Clone

CAT#: RC224959

PPAP2B (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2B (PPAP2B), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406156) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224959 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPAP2B
• Synonyms: Dri42; LPP3; MGC15306; PAP-2b; PAP2-b; PAP2-beta; VCIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224959 representing NM_177414
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAACTACAAGTACGACAAAGCGATCGTCCCGGAGAGCAAGAACGGCGGCAGCCCGGCGCTCAACA
  ACAACCCGAGGAGGAGCGGCAGCAAGCGGGTGCTGCTCATCTGCCTCGACCTCTTCTGCCTCTTCATGGC
  GGGCCTCCCCTTCCTCATCATCGAGACAAGCACCATCAAGCCTTACCACCGAGGGTTTTACTGCAATGAT
  GAGAGCATCAAGTACCCACTGAAAACTGGTGAGACAATAAATGACGCTGTGCTCTGTGCCGTGGGGATCG
  TCATTGCCATCCTCGCGATCATCACGGGGGAATTCTACCGGATCTATTACCTGAAGAAGTCGCGGTCGAC
  GATTCAGAACCCCTACGTGGCAGCACTCTATAAGCAAGTGGGCTGCTTCCTCTTTGGCTGTGCCATCAGC
  CAGTCTTTCACAGACATTGCCAAAGTGTCCATAGGGCGCCTGCGTCCTCACTTCTTGAGTGTCTGCAACC
  CTGATTTCAGCCAGATCAACTGCTCTGAAGGCTACATTCAGAACTACAGATGCAGAGGTGATGACAGCAA
  AGTCCAGGAAGCCAGGAAGTCCTTCTTCTCTGGCCATGCCTCCTTCTCCATGTACACTATGCTGTATTTG
  GTGCTATACCTGCAGGCCCGCTTCACTTGGCGAGGAGCCCGCCTGCTCCGGCCCCTCCTGCAGTTCACCT
  TGATCATGATGGCCTTCTACACGGGACTGTCTCGCGTATCAGACCACAAGCACCATCCCAGTGATGTTCT
  GGCAGGATTTGCTCAAGGAGCCCTGGTGGCCTGCTGCATAGTTTTCTTCGTGTCTGACCTCTTCAAGACT
  AAGACGACGCTCTCCCTGCCTGCCCCTGCTATCCGGAAGGAAATCCTTTCACCTGTGGACATTATTGACA
  GGAACAATCACCACAACATGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224959 representing NM_177414
  Red=Cloning site Green=Tags(s)
  
  MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCND
  ESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAIS
  QSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYL
  VLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKT
  KTTLSLPAPAIRKEILSPVDIIDRNNHHNMM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177414
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177414.1, NP_803133.1
• RefSeq Size: 3243 bp
• RefSeq ORF: 935 bp
• Locus ID: 8613
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• MW: 34.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010]