Lubricin (PRG4) (NM_001127710) Human Tagged ORF Clone

CAT#: RC226376

PRG4 (Myc-DDK-tagged)-Human proteoglycan 4 (PRG4), transcript variant D

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRG4
• Synonyms: CACP; HAPO; JCAP; MSF; SZP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226376 representing NM_001127710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGGAAAACACTTCCCATTTACCTGTTGTTGCTGCTGTCTGTTTTCGTGATTCAGCAAGTTTCAT
  CTCAAGAGCTTTCCTGTAAAGGCCGCTGCTTTGAGTCCTTCGAGAGAGGGAGGGAGTGTGACTGCGACGC
  CCAATGTAAGAAGTATGACAAGTGCTGTCCCGATTATGAGAGTTTCTGTGCAGAAGTAAAAGATAACAAG
  AAGAACAGAACTAAAAAGAAACCTACCCCCAAACCACCAGTTGTAGATGAAGCTGGAAGTGGATTGGACA
  ATGGTGACTTCAAGGTCACAACTCCTGACACGTCTACCACCCAACACAATAAAGTCAGCACATCTCCCAA
  GATCACAACAGCAAAACCAATAAATCCCAGACCCAGTCTTCCACCTAATTCTGATACATCTAAAGAGACG
  TCTTTGACAGTGAATAAAGAGACAACAGTTGAAACTAAAGAAACTACTACAACAAATAAACAGACTTCAA
  CTGATGGAAAAGAGAAGACTACTTCCGCTAAAGAGACACAAAGTATAGAGAAAACATCTGCTAAAGATTT
  AGCACCCACATCTAAAGTGCTGGCTAAACCTACACCCAAAGCTGAAACTACAACCAAAGGCCCTGCTCTC
  ACCACTCCCAAGGAGCCCACGCCCACCACTCCCAAGGAGCCTGCATCTACCACACCCAAAGAGCCCACAC
  CTACCACCATCAAGTCTGCACCCACCACCCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCAC
  CACTCCCAAGGAGCCTGCACCCACCACCACCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCC
  ACCACCACCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCCAAGGAGCCTGCACCCA
  CCACCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTACACC
  CACCACTCCCAAGGAGCCTGCACCCACCACCAAGGAGCCTGCACCCACCACTCCCAAAGAGCCTGCACCC
  ACTGCCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCAC
  CCACCACCACCAAGGAGCCTTCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACC
  CACCACTACCAAGGAGCCTGCACCCACCACTACCAAGTCTGCACCCACCACTCCCAAGGAGCCTTCACCC
  ACCACCACCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCCCCAAGAAGCCTGCCC
  CAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAACCTGCACCCACCACCACCAAGAAGCCTGC
  ACCCACCACTCCCAAAGAGCCTGCCCCAACTACCCCCAAGGAGACTGCACCCACCACCCCCAAGAAGCTC
  ACGCCCACCACCCCCGAGAAGCTCGCACCCACCACCCCTGAGAAGCCCGCACCCACCACCCCTGAGGAGC
  TCGCACCCACCACCCCTGAGGAGCCCACACCCACCACCCCTGAGGAGCCTGCTCCCACCACTCCCAAGGC
  AGCGGCTCCCAACACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAG
  GAGCCTGCTCCAACTACCCCTAAGGAGACTGCTCCAACTACCCCTAAAGGGACTGCTCCAACTACCCTCA
  AGGAACCTGCACCCACTACTCCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGAGCC
  CACATCCACCACCTGTGACAAGCCCGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCCTAAGGAG
  CCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGGGACTGCTCCAACTACCCTCAAGG
  AACCTGCACCCACTACTCCCAAGAAGCCTGCCCCCAAGGAGCTTGCACCCACCACCACCAAGGGGCCCAC
  ATCCACCACCTCTGACAAGCCTGCTCCAACTACACCTAAGGAGACTGCTCCAACTACCCCCAAGGAGCCT
  GCACCCACTACCCCCAAGAAGCCTGCTCCAACTACTCCTGAGACACCTCCTCCAACCACTTCAGAGGTCT
  CTACTCCAACTACCACCAAGGAGCCTACCACTATCCACAAAAGCCCTGATGAATCAACTCCTGAGCTTTC
  TGCAGAACCCACACCAAAAGCTCTTGAAAACAGTCCCAAGGAACCTGGTGTACCTACAACTAAGACTCCT
  GCAGCGACTAAACCTGAAATGACTACAACAGCTAAAGACAAGACAACAGAAAGAGACTTACGTACTACAC
  CTGAAACTACAACTGCTGCACCTAAGATGACAAAAGAGACAGCAACTACAACAGAAAAAACTACCGAATC
  CAAAATAACAGCTACAACCACACAAGTAACATCTACCACAACTCAAGATACCACACCATTCAAAATTACT
  ACTCTTAAAACAACTACTCTTGCACCCAAAGTAACTACAACAAAAAAGACAATTACTACCACTGAGATTA
  TGAACAAACCTGAAGAAACAGCTAAACCAAAAGACAGAGCTACTAATTCTAAAGCGACAACTCCTAAACC
  TCAAAAGCCAACCAAAGCACCCAAAAAACCCACTTCTACCAAAAAGCCAAAAACAATGCCTAGAGTGAGA
  AAACCAAAGACGACACCAACTCCCCGCAAGATGACATCAACAATGCCAGAATTGAACCCTACCTCAAGAA
  TAGCAGAAGCCATGCTCCAAACCACCACCAGACCTAACCAAACTCCAAACTCCAAACTAGTTGAAGTAAA
  TCCAAAGAGTGAAGATGCAGGTGGTGCTGAAGGAGAAACACCTCATATGCTTCTCAGGCCCCATGTGTTC
  ATGCCTGAAGTTACTCCCGACATGGATTACTTACCGAGAGTACCCAATCAAGGCATTATCATCAATCCCA
  TGCTTTCCGATGAGACCAATATATGCAATGGTAAGCCAGTAGATGGACTGACTACTTTGCGCAATGGGAC
  ATTAGTTGCATTCCGAGGTCATTATTTCTGGATGCTAAGTCCATTCAGTCCACCATCTCCAGCTCGCAGA
  ATTACTGAAGTTTGGGGTATTCCTTCCCCCATTGATACTGTTTTTACTAGGTGCAACTGTGAAGGAAAAA
  CTTTCTTCTTTAAGGATTCTCAGTACTGGCGTTTTACCAATGATATAAAAGATGCAGGGTACCCCAAACC
  AATTTTCAAAGGATTTGGAGGACTAACTGGACAAATAGTGGCAGCGCTTTCAACAGCTAAATATAAGAAC
  TGGCCTGAATCTGTGTATTTTTTCAAGAGAGGTGGCAGCATTCAGCAGTATATTTATAAACAGGAACCTG
  TACAGAAGTGCCCTGGAAGAAGGCCTGCTCTAAATTATCCAGTGTATGGAGAAACGACACAGGTTAGGAG
  ACGTCGCTTTGAACGTGCTATAGGACCTTCTCAAACACACACCATCAGAATTCAATATTCACCTGCCAGA
  CTGGCTTATCAAGACAAAGGTGTCCTTCATAATGAAGTTAAAGTGAGTATACTGTGGAGAGGACTTCCAA
  ATGTGGTTACCTCAGCTATATCACTGCCCAACATCAGAAAACCTGACGGCTATGATTACTATGCCTTTTC
  TAAAGATCAATACTATAACATTGATGTGCCTAGTAGAACAGCAAGAGCAATTACTACTCGTTCTGGGCAG
  ACCTTATCCAAAGTCTGGTACAACTGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226376 representing NM_001127710
  Red=Cloning site Green=Tags(s)
  
  MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVKDNK
  KNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPSLPPNSDTSKET
  SLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTSKVLAKPTPKAETTTKGPAL
  TTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTTKEPAPTTPKEPAP
  TTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAP
  TAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTTPKEPSP
  TTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTTPKEPAPTTPKETAPTTPKKL
  TPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPK
  EPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTCDKPAPTTPKGTAPTTPKE
  PAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPKETAPTTPKEP
  APTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKALENSPKEPGVPTTKTP
  AATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQDTTPFKIT
  TLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTKAPKKPTSTKKPKTMPRVR
  KPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDAGGAEGETPHMLLRPHVF
  MPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGTLVAFRGHYFWMLSPFSPPSPARR
  ITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKPIFKGFGGLTGQIVAALSTAKYKN
  WPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRRRRFERAIGPSQTHTIRIQYSPAR
  LAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQ
  TLSKVWYNCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127710
• ORF Size: 3810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001127710.1, NM_001127710.2, NP_001121182.1
• RefSeq ORF: 3813
• Locus ID: 10216
• MW: 136.4 kDa
• Gene Summary: The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]