Lubricin (PRG4) (NM_001127708) Human Tagged ORF Clone

CAT#: RC226389

  • TrueORF®

PRG4 (Myc-DDK-tagged)-Human proteoglycan 4 (PRG4), transcript variant B. Note: ORF is codon optimized


  "NM_001127708" in other vectors (2)

Reconstitution Protocol

USD 1,430.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PRG4
Synonyms CACP; HAPO; JCAP; MSF; SZP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC226389 ORF sequence, codon optimized.
Due to the complexity of NM_001127708, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTGGAAGACCCTGCCAATTTATCTGCTCCTTCTGCTGAGCGTCTTTGTGATACAGCAGGTGAGTA
GTCAGGAGCTCTCATGCAAGGGCAGGTGTTTCGAGTCTTTCGAGCGCGGACGGGAGTGCGATTGCGACGC
TCAGTGCAAAAAGTATGACAAGTGCTGCCCTGACTACGAAAGCTTTTGCGCGGAGGTTCACAACCCCACA
TCTCCGCCCTCAAGCAAAAAGGCCCCACCGCCTTCCGGCGCCTCCCAGACCATTAAGAGTACAACCAAAA
GGAGCCCTAAACCACCAAACAAGAAAAAAACAAAAAAAGTTATAGAGTCAGAGGAAATCACCGAAGAGCA
TAGTGTCTCCGAGAACCAGGAAAGTTCAAGCTCATCCAGCTCCTCCTCATCATCAAGTACCATCCGAAAA
ATCAAGAGCTCCAAAAATTCCGCCGCAAATAGAGAGTTGCAGAAGAAGCTGAAAGTGAAGGATAACAAAA
AAAACAGAACAAAAAAGAAACCCACCCCTAAACCACCGGTTGTGGATGAGGCCGGCTCTGGGCTGGACAA
TGGAGACTTTAAAGTCACCACTCCAGACACGTCAACCACACAACACAACAAAGTAAGCACCTCACCAAAG
ATAACCACAGCCAAGCCCATTAATCCACGGCCATCCTTGCCTCCTAACAGCGACACATCAAAAGAAACTT
CTTTGACCGTGAACAAGGAAACCACGGTCGAGACCAAGGAAACTACAACCACTAATAAGCAGACCTCCAC
CGACGGGAAGGAGAAGACCACTTCAGCTAAAGAGACACAGTCCATAGAGAAAACTTCTGCAAAAGATCTG
GCACCAACGTCTAAGGTGCTCGCTAAACCGACTCCGAAAGCAGAAACCACCACAAAGGGTCCCGCTCTCA
CTACTCCAAAGGAGCCAACACCTACCACACCCAAAGAACCCGCTTCAACCACACCTAAGGAGCCCACGCC
CACCACAATTAAGTCCGCCCCGACTACACCCAAGGAGCCTGCCCCGACCACAACAAAATCTGCCCCTACT
ACCCCGAAGGAGCCCGCTCCAACAACCACTAAGGAGCCCGCACCGACTACCCCTAAGGAACCCGCCCCTA
CGACGACAAAGGAACCAGCTCCAACCACGACCAAGAGCGCTCCCACCACCCCTAAGGAGCCTGCCCCCAC
AACTCCTAAGAAGCCGGCTCCTACAACCCCGAAGGAACCAGCCCCAACCACGCCAAAAGAACCCACTCCA
ACAACCCCTAAAGAGCCAGCACCTACAACTAAAGAGCCTGCTCCGACAACCCCTAAGGAGCCTGCTCCTA
CAGCCCCCAAAAAACCAGCGCCCACCACGCCCAAGGAGCCCGCTCCTACAACCCCCAAAGAACCAGCCCC
AACCACTACTAAGGAACCGTCACCCACTACCCCAAAAGAACCGGCACCCACTACCACAAAATCAGCCCCC
ACTACCACAAAAGAGCCAGCGCCAACCACTACCAAAAGTGCGCCTACTACACCGAAAGAGCCTAGCCCAA
CCACTACAAAAGAACCGGCGCCCACAACTCCCAAAGAACCCGCTCCGACCACCCCTAAGAAACCAGCACC
TACTACACCCAAGGAACCCGCCCCTACCACACCAAAAGAGCCAGCCCCCACCACAACGAAAAAACCCGCG
CCTACCACACCCAAAGAACCCGCCCCCACTACTCCAAAGGAGACCGCTCCTACCACGCCAAAGAAACTGA
CCCCCACTACGCCGGAGAAACTGGCACCAACCACCCCAGAGAAACCCGCCCCCACTACTCCCGAGGAACT
CGCTCCGACGACACCCGAAGAACCCACGCCTACAACGCCTGAGGAGCCTGCCCCTACAACCCCAAAAGCC
GCAGCGCCAAATACCCCCAAGGAACCTGCGCCGACTACTCCTAAGGAGCCCGCACCCACAACACCAAAGG
AGCCAGCGCCGACAACTCCTAAAGAGACCGCTCCAACTACGCCTAAAGGGACTGCACCTACGACATTGAA
AGAACCTGCGCCTACGACCCCTAAGAAACCTGCTCCCAAGGAACTCGCTCCCACTACCACAAAAGAGCCA
ACGTCCACCACATGCGATAAACCAGCCCCGACCACTCCCAAGGGTACGGCTCCAACCACACCCAAGGAAC
CTGCCCCAACTACACCGAAGGAGCCCGCCCCAACGACCCCAAAGGGGACTGCTCCTACTACGTTGAAAGA
ACCAGCCCCCACAACACCAAAAAAACCCGCCCCCAAAGAGCTCGCCCCTACAACTACAAAGGGCCCTACT
AGTACCACCAGTGACAAACCCGCACCAACAACCCCGAAAGAGACTGCCCCTACCACACCTAAGGAGCCGG
CGCCTACCACCCCCAAAAAACCTGCCCCGACAACCCCTGAAACCCCTCCCCCAACAACAAGCGAGGTGTC
AACCCCCACGACTACCAAGGAGCCCACTACCATTCACAAATCTCCTGATGAGAGCACCCCAGAGTTGAGT
GCGGAGCCCACTCCGAAAGCTCTGGAAAATAGTCCAAAAGAGCCAGGTGTCCCCACTACAAAGACTCCTG
CTGCTACCAAGCCCGAAATGACAACTACCGCCAAGGATAAAACCACCGAGCGAGATCTGCGGACAACACC
CGAGACTACTACAGCCGCACCAAAAATGACGAAAGAAACAGCCACAACCACCGAAAAAACCACAGAGTCC
AAGATAACCGCCACCACCACGCAAGTGACATCTACCACAACACAGGATACTACGCCCTTCAAAATCACCA
CACTTAAAACAACTACACTGGCACCAAAGGTGACAACCACTAAGAAGACAATAACAACGACTGAGATAAT
GAATAAGCCCGAAGAGACAGCGAAACCAAAGGACAGAGCTACCAATTCCAAAGCCACCACCCCAAAGCCA
CAAAAACCAACAAAGGCACCCAAAAAGCCTACCAGTACCAAAAAACCAAAGACCATGCCTAGAGTACGGA
AGCCCAAGACAACACCCACACCGCGCAAAATGACGAGCACTATGCCAGAACTTAATCCCACATCCCGGAT
CGCAGAAGCTATGTTGCAGACCACTACCCGCCCCAATCAGACACCGAACAGTAAACTCGTCGAAGTCAAC
CCCAAATCAGAGGATGCCGGAGGCGCCGAAGGGGAGACTCCACATATGCTCCTGCGACCGCATGTTTTTA
TGCCTGAAGTGACCCCTGACATGGATTACCTTCCTAGGGTCCCTAATCAGGGTATCATCATCAATCCTAT
GCTCAGTGATGAGACCAATATTTGTAACGGGAAACCAGTGGATGGACTGACAACACTGCGCAACGGCACA
CTGGTTGCTTTTCGCGGTCATTATTTTTGGATGCTGTCCCCATTCTCACCACCATCCCCAGCTCGAAGGA
TCACAGAGGTGTGGGGCATTCCTTCTCCAATTGACACCGTTTTCACCCGCTGCAATTGCGAAGGAAAAAC
CTTCTTTTTCAAAGACAGTCAGTATTGGCGATTTACAAATGATATCAAAGACGCAGGGTATCCCAAGCCG
ATCTTCAAAGGCTTCGGAGGCCTGACAGGCCAGATTGTTGCCGCTCTCTCCACCGCTAAGTACAAGAACT
GGCCTGAATCCGTGTATTTCTTTAAGAGAGGGGGAAGCATTCAACAATATATCTATAAGCAGGAGCCCGT
GCAGAAATGCCCAGGCAGACGGCCCGCTCTGAACTACCCAGTTTACGGCGAGACTACCCAGGTAAGGAGG
AGGCGCTTCGAGAGAGCTATTGGGCCCTCCCAAACCCACACAATACGGATACAGTACTCTCCGGCAAGAC
TGGCATACCAGGACAAGGGAGTGCTCCACAACGAGGTAAAGGTTAGCATATTGTGGCGGGGCTTGCCAAA
CGTTGTAACATCTGCCATAAGCCTGCCCAACATTCGCAAGCCTGACGGATACGACTACTATGCATTTAGC
AAAGATCAGTATTACAATATAGACGTGCCTTCCAGAACTGCCCGCGCAATTACCACCAGGTCCGGCCAGA
CACTCTCCAAGGTGTGGTATAATTGCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC226389 representing NM_001127708
Red=Cloning site Green=Tags(s)

MAWKTLPIYLLLLLSVFVIQQVSSQELSCKGRCFESFERGRECDCDAQCKKYDKCCPDYESFCAEVHNPT
SPPSSKKAPPPSGASQTIKSTTKRSPKPPNKKKTKKVIESEEITEEHSVSENQESSSSSSSSSSSSTIRK
IKSSKNSAANRELQKKLKVKDNKKNRTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPK
ITTAKPINPRPSLPPNSDTSKETSLTVNKETTVETKETTTTNKQTSTDGKEKTTSAKETQSIEKTSAKDL
APTSKVLAKPTPKAETTTKGPALTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPT
TPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTP
TTPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAP
TTTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPA
PTTPKEPAPTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEPAPTTPKA
AAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKEP
TSTTCDKPAPTTPKGTAPTTPKEPAPTTPKEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPT
STTSDKPAPTTPKETAPTTPKEPAPTTPKKPAPTTPETPPPTTSEVSTPTTTKEPTTIHKSPDESTPELS
AEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTTERDLRTTPETTTAAPKMTKETATTTEKTTES
KITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKP
QKPTKAPKKPTSTKKPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVN
PKSEDAGGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPNQGIIINPMLSDETNICNGKPVDGLTTLRNGT
LVAFRGHYFWMLSPFSPPSPARRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDIKDAGYPKP
IFKGFGGLTGQIVAALSTAKYKNWPESVYFFKRGGSIQQYIYKQEPVQKCPGRRPALNYPVYGETTQVRR
RRFERAIGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFS
KDQYYNIDVPSRTARAITTRSGQTLSKVWYNCP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001127708
ORF Size 4089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001127708.1, NM_001127708.2, NP_001121180.1
RefSeq Size 4927
RefSeq ORF 4092
Locus ID 10216
MW 146.5 kDa
Gene Summary The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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