Lubricin (PRG4) (NM_001127708) Human Untagged Clone
CAT#: SC323276
PRG4 (untagged)-Human proteoglycan 4 (PRG4), transcript variant B
"NM_001127708" in other vectors (2)
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PRG4 |
Synonyms | CACP; HAPO; JCAP; MSF; SZP |
Vector | pCMV6-Entry |
Sequence Data |
>SC323276 representing NM_001127708.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCATGGAAAACACTTCCCATTTACCTGTTGTTGCTGCTGTCTGTTTTCGTGATTCAGCAAGTTTCA TCTCAAGAGCTTTCCTGTAAAGGCCGCTGCTTTGAGTCCTTCGAGAGAGGGAGGGAGTGTGACTGCGAC GCCCAATGTAAGAAGTATGACAAGTGCTGTCCCGATTATGAGAGTTTCTGTGCAGAAGTGCATAATCCC ACATCACCACCATCTTCAAAGAAAGCACCTCCACCTTCAGGAGCATCTCAAACCATCAAATCAACAACC AAACGTTCACCCAAACCACCAAACAAGAAGAAGACTAAGAAAGTTATAGAATCAGAGGAAATAACAGAA GAACATTCTGTTTCTGAAAATCAAGAGTCCTCCTCCTCCTCCTCCTCTTCCTCTTCTTCTTCAACAATT CGGAAAATCAAGTCTTCCAAAAATTCAGCTGCTAATAGAGAATTACAGAAGAAACTCAAAGTAAAAGAT AACAAGAAGAACAGAACTAAAAAGAAACCTACCCCCAAACCACCAGTTGTAGATGAAGCTGGAAGTGGA TTGGACAATGGTGACTTCAAGGTCACAACTCCTGACACGTCTACCACCCAACACAATAAAGTCAGCACA TCTCCCAAGATCACAACAGCAAAACCAATAAATCCCAGACCCAGTCTTCCACCTAATTCTGATACATCT AAAGAGACGTCTTTGACAGTGAATAAAGAGACAACAGTTGAAACTAAAGAAACTACTACAACAAATAAA CAGACTTCAACTGATGGAAAAGAGAAGACTACTTCCGCTAAAGAGACACAAAGTATAGAGAAAACATCT GCTAAAGATTTAGCACCCACATCTAAAGTGCTGGCTAAACCTACACCCAAAGCTGAAACTACAACCAAA GGCCCTGCTCTCACCACTCCCAAGGAGCCCACGCCCACCACTCCCAAGGAGCCTGCATCTACCACACCC AAAGAGCCCACACCTACCACCATCAAGTCTGCACCCACCACCCCCAAGGAGCCTGCACCCACCACCACC AAGTCTGCACCCACCACTCCCAAGGAGCCTGCACCCACCACCACCAAGGAGCCTGCACCCACCACTCCC AAGGAGCCTGCACCCACCACCACCAAGGAGCCTGCACCCACCACCACCAAGTCTGCACCCACCACTCCC AAGGAGCCTGCACCCACCACCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACT CCCAAGGAGCCTACACCCACCACTCCCAAGGAGCCTGCACCCACCACCAAGGAGCCTGCACCCACCACT CCCAAAGAGCCTGCACCCACTGCCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACC ACTCCCAAGGAGCCTGCACCCACCACCACCAAGGAGCCTTCACCCACCACTCCCAAGGAGCCTGCACCC ACCACCACCAAGTCTGCACCCACCACTACCAAGGAGCCTGCACCCACCACTACCAAGTCTGCACCCACC ACTCCCAAGGAGCCTTCACCCACCACCACCAAGGAGCCTGCACCCACCACTCCCAAGGAGCCTGCACCC ACCACCCCCAAGAAGCCTGCCCCAACTACCCCCAAGGAGCCTGCACCCACCACTCCCAAGGAACCTGCA CCCACCACCACCAAGAAGCCTGCACCCACCACTCCCAAAGAGCCTGCCCCAACTACCCCCAAGGAGACT GCACCCACCACCCCCAAGAAGCTCACGCCCACCACCCCCGAGAAGCTCGCACCCACCACCCCTGAGAAG CCCGCACCCACCACCCCTGAGGAGCTCGCACCCACCACCCCTGAGGAGCCCACACCCACCACCCCTGAG GAGCCTGCTCCCACCACTCCCAAGGCAGCGGCTCCCAACACCCCTAAGGAGCCTGCTCCAACTACCCCT AAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGACTGCTCCAACTACC CCTAAAGGGACTGCTCCAACTACCCTCAAGGAACCTGCACCCACTACTCCCAAGAAGCCTGCCCCCAAG GAGCTTGCACCCACCACCACCAAGGAGCCCACATCCACCACCTGTGACAAGCCCGCTCCAACTACCCCT AAGGGGACTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACCCCTAAGGAGCCTGCTCCAACTACC CCTAAGGGGACTGCTCCAACTACCCTCAAGGAACCTGCACCCACTACTCCCAAGAAGCCTGCCCCCAAG GAGCTTGCACCCACCACCACCAAGGGGCCCACATCCACCACCTCTGACAAGCCTGCTCCAACTACACCT AAGGAGACTGCTCCAACTACCCCCAAGGAGCCTGCACCCACTACCCCCAAGAAGCCTGCTCCAACTACT CCTGAGACACCTCCTCCAACCACTTCAGAGGTCTCTACTCCAACTACCACCAAGGAGCCTACCACTATC CACAAAAGCCCTGATGAATCAACTCCTGAGCTTTCTGCAGAACCCACACCAAAAGCTCTTGAAAACAGT CCCAAGGAACCTGGTGTACCTACAACTAAGACTCCTGCAGCGACTAAACCTGAAATGACTACAACAGCT AAAGACAAGACAACAGAAAGAGACTTACGTACTACACCTGAAACTACAACTGCTGCACCTAAGATGACA AAAGAGACAGCAACTACAACAGAAAAAACTACCGAATCCAAAATAACAGCTACAACCACACAAGTAACA TCTACCACAACTCAAGATACCACACCATTCAAAATTACTACTCTTAAAACAACTACTCTTGCACCCAAA GTAACTACAACAAAAAAGACAATTACTACCACTGAGATTATGAACAAACCTGAAGAAACAGCTAAACCA AAAGACAGAGCTACTAATTCTAAAGCGACAACTCCTAAACCTCAAAAGCCAACCAAAGCACCCAAAAAA CCCACTTCTACCAAAAAGCCAAAAACAATGCCTAGAGTGAGAAAACCAAAGACGACACCAACTCCCCGC AAGATGACATCAACAATGCCAGAATTGAACCCTACCTCAAGAATAGCAGAAGCCATGCTCCAAACCACC ACCAGACCTAACCAAACTCCAAACTCCAAACTAGTTGAAGTAAATCCAAAGAGTGAAGATGCAGGTGGT GCTGAAGGAGAAACACCTCATATGCTTCTCAGGCCCCATGTGTTCATGCCTGAAGTTACTCCCGACATG GATTACTTACCGAGAGTACCCAATCAAGGCATTATCATCAATCCCATGCTTTCCGATGAGACCAATATA TGCAATGGTAAGCCAGTAGATGGACTGACTACTTTGCGCAATGGGACATTAGTTGCATTCCGAGGTCAT TATTTCTGGATGCTAAGTCCATTCAGTCCACCATCTCCAGCTCGCAGAATTACTGAAGTTTGGGGTATT CCTTCCCCCATTGATACTGTTTTTACTAGGTGCAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCT CAGTACTGGCGTTTTACCAATGATATAAAAGATGCAGGGTACCCCAAACCAATTTTCAAAGGATTTGGA GGACTAACTGGACAAATAGTGGCAGCGCTTTCAACAGCTAAATATAAGAACTGGCCTGAATCTGTGTAT TTTTTCAAGAGAGGTGGCAGCATTCAGCAGTATATTTATAAACAGGAACCTGTACAGAAGTGCCCTGGA AGAAGGCCTGCTCTAAATTATCCAGTGTATGGAGAAACGACACAGGTTAGGAGACGTCGCTTTGAACGT GCTATAGGACCTTCTCAAACACACACCATCAGAATTCAATATTCACCTGCCAGACTGGCTTATCAAGAC AAAGGTGTCCTTCATAATGAAGTTAAAGTGAGTATACTGTGGAGAGGACTTCCAAATGTGGTTACCTCA GCTATATCACTGCCCAACATCAGAAAACCTGACGGCTATGATTACTATGCCTTTTCTAAAGATCAATAC TATAACATTGATGTGCCTAGTAGAACAGCAAGAGCAATTACTACTCGTTCTGGGCAGACCTTATCCAAA GTCTGGTACAACTGTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites |
SgfI-MluI
Plasmid Map
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ACCN | NM_001127708 |
Insert Size | 4089 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127708.1, NP_001121180.1 |
RefSeq Size | 4936 bp |
RefSeq ORF | 4089 bp |
Locus ID | 10216 |
UniProt ID | Q92954 |
Cytogenetics | 1q31.1 |
MW | 146.5 kDa |
Gene Summary | The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (B, also known as V0a) lacks an alternate in-frame exon in the 5' coding region, compared to variant A. The resulting protein (isoform B) is shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
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