NFATC4 (NM_001136022) Human Tagged ORF Clone

CAT#: RC226608

NFATC4 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427771) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226608 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NFATC4 protein (Cat# TP326608). The protein was produced from HEK293T cells transfected with NFATC4 cDNA clone (Cat# RC226608) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFATC4
• Synonyms: NF-AT3; NF-ATC4; NFAT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226608 representing NM_001136022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAACCACCCTCCCATCTCTCCTACCCGCCAGCCTCGCCAGTATCTCCCACCGAGTCACGAATCTCC
  CATCTAACTCCCTCTCACACAACCCAGGCCTCTCCAAGCCTGACTTTCCCGGAAACTCCAGTCCAGGTCT
  TCCTTCCTCCTCCAGCCCAGGCCGGGACCTGGGGGCTCCTGCCGGATCCATGGGGGCGGCCAGCTGCGAG
  GATGAGGAGCTGGAATTTAAGCTGGTGTTCGGGGAGGAAAAGGAGGCCCCCCCGCTGGGCGCGGGGGGAT
  TGGGGGAAGAACTGGACTCAGAGGATGCCCCGCCATGCTGCCGTCTGGCCTTGGGAGAGCCCCCTCCCTA
  TGGCGCTGCACCTATCGGTATTCCCCGACCTCCACCCCCTCGGCCTGGCATGCATTCGCCACCGCCGCGA
  CCAGCCCCCTCACCTGGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGAGGCTGGGAGGACCAGGAGGGG
  GTGCTGGGGGTGCTGGGGGTGGCCGTGTTCTCGAGTGTCCCAGCATCCGCATCACCTCCATCTCTCCCAC
  GCCGGAGCCGCCAGCAGCGCTGGAGGACAACCCTGATGCCTGGGGGGACGGCTCTCCTAGAGATTACCCC
  CCACCAGAAGGCTTTGGGGGCTACAGAGAAGCAGGGGGCCAGGGTGGGGGGGCCTTCTTCAGCCCAAGCC
  CTGGCAGCAGCAGCCTGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGACGAGGCAGCCCTGTATGCAGC
  CTGCGACGAGGTGGAGTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGCCTGGGCTCCCCGCTGCCCTCG
  CCCCGGGCCTCCCCTCGGCCATGGACCCCCGAAGATCCCTGGAGCCTGTATGGTCCAAGCCCCGGAGGCC
  GAGGGCCAGAGGATAGCTGGCTACTCCTCAGTGCTCCTGGGCCCACCCCAGCCTCCCCGCGGCCTGCCTC
  TCCATGTGGCAAGCGGCGCTATTCCAGCTCGGGAACCCCATCTTCAGCCTCCCCAGCTCTGTCCCGCCGT
  GGCAGCCTGGGGGAAGAGGGGTCTGAGCCACCTCCACCACCCCCATTGCCTCTGGCCCGGGACCCGGGCT
  CCCCTGGTCCCTTTGACTATGTGGGGGCCCCACCAGCTGAGAGCATCCCTCAGAAGACACGGCGGACTTC
  CAGCGAGCAGGCAGTGGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGCAATGGGAAGCTGCCCTTGGGA
  GCAGAGGAGTCTGTGGCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTGGCATGGACTACCTGGCAGTGC
  CCTCCCCACTCGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCTATCTTCAGGACCTCTGCCCTACC
  CCCACTGGACTGGCCTCTGCCCAGCCAATATGAGCAGCTGGAGCTGAGGATCGAGGTACAGCCTAGAGCC
  CACCACCGGGCCCACTATGAGACAGAAGGCAGCCGTGGAGCTGTCAAAGCTGCCCCTGGCGGTCACCCCG
  TAGTCAAGCTCCTAGGCTACAGTGAGAAGCCACTGACCCTACAGATGTTCATCGGCACTGCAGATGAAAG
  GAACCTGCGGCCTCATGCCTTCTATCAGGTGCACCGTATCACAGGCAAGATGGTGGCCACGGCCAGCTAT
  GAAGCCGTAGTCAGTGGCACCAAGGTGTTGGAGATGACTCTGCTGCCTGAGAACAACATGGCGGCCAACA
  TTGACTGCGCGGGAATCCTGAAGCTTCGGAATTCAGACATTGAGCTTCGGAAGGGTGAGACGGACATCGG
  GCGCAAAAACACACGTGTACGGCTGGTGTTCCGGGTACACGTGCCCCAGGGCGGCGGGAAGGTCGTCTCA
  GTACAGGCAGCATCGGTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGGAGCTGCCCCAGGTGGAGGCCT
  ACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGCGAGGAACTGGTACTGACTGGCTCCAACTTCCTGCCAGA
  CTCCAAGGTGGTGTTCATTGAGAGGGGTCCTGATGGGAAGCTGCAATGGGAGGAGGAGGCCACAGTGAAC
  CGACTGCAGAGCAACGAGGTGACGCTGACCCTGACTGTCCCCGAGTACAGCAACAAGAGGGTTTCCCGGC
  CAGTCCAGGTCTACTTTTATGTCTCCAATGGGCGGAGGAAACGCAGTCCTACCCAGAGTTTCAGGTTTCT
  GCCTGTGATCTGCAAAGAGGAGCCCCTACCGGACTCATCTCTGCGGGGTTTCCCTTCAGCATCGGCAACC
  CCCTTTGGCACTGACATGGACTTCTCACCACCCAGGCCCCCCTACCCCTCCTATCCCCATGAAGACCCTG
  CTTGCGAAACTCCTTACCTATCAGAAGGCTTCGGCTATGGCATGCCCCCTCTGTACCCCCAGACGGGGCC
  CCCACCATCCTACAGACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGTACCACAGGTTGTGCCCAACCA
  CCTGCAGTTTCCTTCCTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAGGGCGGGGCTCCTCTTTCTCCC
  TGGGGCTGCCATTCTCTCCGCCAGCCCCCTTTCGGCCGCCTCCTCTTCCTGCATCCCCACCGCTTGAAGG
  CCCCTTCCCTTCCCAGAGTGATGTGCATCCCCTACCTGCTGAGGGATACAATAAGGTAGGGCCAGGCTAT
  GGCCCTGGGGAGGGGGCTCCGGAGCAGGAGAAATCCAGGGGTGGCTACAGCAGCGGCTTCCGAGACAGTG
  TCCCTATCCAGGGTATCACGCTGGAGGAAGGTGGGTGTGGGACTGGGGGCTGTGAGTGTGAGTGTGTGCA
  AGAGATTGCTCTGCATGTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226608 representing NM_001136022
  Red=Cloning site Green=Tags(s)
  
  MITTLPSLLPASLASISHRVTNLPSNSLSHNPGLSKPDFPGNSSPGLPSSSSPGRDLGAPAGSMGAASCE
  DEELEFKLVFGEEKEAPPLGAGGLGEELDSEDAPPCCRLALGEPPPYGAAPIGIPRPPPPRPGMHSPPPR
  PAPSPGTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGDGSPRDYP
  PPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFGLGSPLPS
  PRASPRPWTPEDPWSLYGPSPGGRGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSASPALSRR
  GSLGEEGSEPPPPPPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASCNGKLPLG
  AEESVAPPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELRIEVQPRA
  HHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGKMVATASY
  EAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQGGGKVVS
  VQAASVPIECSQRSAQELPQVEAYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQWEEEATVN
  RLQSNEVTLTLTVPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRGFPSASAT
  PFGTDMDFSPPRPPYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRGTTGCAQP
  PAVSFLPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGYNKVGPGY
  GPGEGAPEQEKSRGGYSSGFRDSVPIQGITLEEGGCGTGGCECECVQEIALHVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136022
• ORF Size: 2892 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136022.1, NM_001136022.2, NP_001129494.1
• RefSeq ORF: 2895 bp
• Locus ID: 4776
• Cytogenetics: 14q12
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 101.3 kDa
• Gene Summary: 'This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]'