ANO6 (NM_001142678) Human Tagged ORF Clone

CAT#: RC226624

ANO6 (Myc-DDK-tagged)-Human anoctamin 6 (ANO6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANO6
• Synonyms: BDPLT7; SCTS; TMEM16F
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226624 representing NM_001142678
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTGTGCTGCTGTGTTGGAAAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTC
  AGCATGATTTTCGAACCCCGGAGTTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGG
  CCAGCGAAGAATTGACTTTGTTCTAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACA
  AATGAAAAACAAAGGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAG
  CAACAAGATCAGTATTGGATGACAAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTAC
  GTATGCTGAGATAATGCACATCAAATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTT
  GGTACACTCAACTGGTTTACCAAAGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTT
  TCACTGCCCCATTTGAGAAGAACCGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCC
  AGCCACCAGAAGCCGCATTGTTTACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGC
  AAGTTTGGGATCAACAGACTTGTAAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAAT
  TCCGCCGTCAGTCAGAGGATCCCAGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCC
  TCGAAGCATATACAAAAAGCAGCCCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTAC
  TTTGCTTGGCTGGGCTATTACACTCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCT
  ATGGATATCTTAATCAAGATAACTGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGAT
  CATAATGTGTCCTCAGTGTGATAGGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAG
  AAATTGTGCATCTTCGACAGTTTTGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTGT
  TTTTGGAGTTTTGGAAGCGACGCCAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAGGA
  AGAACAAGCCCGACCAGAATACGAAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAAGAA
  GAACGCATTCCCTTTACTGCCTGGGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTTTTCT
  GGATCCTATTGATCATCGCTTCAGTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTATTTTC
  TGCAAAACTTCCCAAGAACATTAATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACAGCCACG
  TCCATCACGGCCTCCATCATCAGCTTTATAATTATCATGATTCTGAACACCATATATGAAAAAGTGGCAA
  TTATGATTACTAACTTCGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATGAAGATGTT
  CTTATTCCAGTTTGTCAACTACTACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAATTTGTAGGC
  TATCCAGGAGACCCAGTTTATTGGTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGTGGCTGTCTTC
  TTGAACTGACAACTCAGCTGACAATAATCATGGGAGGAAAAGCAATCTGGAATAACATACAAGAAGTATT
  ATTGCCCTGGATCATGAATCTAATTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAGATAACCCCACGA
  TGGGAACAGGACTACCATCTGCAGCCTATGGGCAAACTGGGATTATTTTATGAATATCTTGAAATGATTA
  TTCAGTTTGGGTTCGTCACCTTATTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTGGCTCTCGTGAACAA
  TATATTGGAAATAAGAGTGGACGCATGGAAACTGACCACCCAGTTTAGACGCCTGGTACCAGAGAAAGCC
  CAAGACATTGGAGCATGGCAGCCCATCATGCAAGGAATAGCAATTCTGGCTGTGGTGACCAATGCCATGA
  TCATAGCTTTCACGTCGGACATGATCCCCCGCCTAGTGTACTACTGGTCCTTCTCCGTCCCTCCCTACGG
  GGACCACACTTCCTACACCATGGAAGGGTACATCAACAACACTCTCTCCATCTTCAAAGTCGCAGACTTC
  AAAAACAAAAGCAAGGGAAACCCGTACTCTGACCTGGGTAACCATACCACATGCAGGTATCGTGATTTCC
  GATACCCACCTGGACACCCCCAGGAGTATAAACACAACATCTACTATTGGCATGTGATTGCAGCCAAGCT
  GGCTTTTATCATTGTCATGGAGCACGTCATCTACTCTGTGAAATTTTTCATTTCATATGCAATTCCCGAT
  GTATCAAAACGCACAAAGAGCAAGATCCAGAGAGAAAAATACCTAACCCAAAAGCTTCTTCATGAGAATC
  ACCTCAAAGATATGACGAAAAATATGGGGGTGATAGCTGAGCGGATGATAGAAGCAGTAGATAACAATTT
  ACGGCCAAAATCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226624 representing NM_001142678
  Red=Cloning site Green=Tags(s)
  
  MFCAAVLENLGQTIVPDLGSLESQHDFRTPEFEEFNGKPDSLFFNDGQRRIDFVLVYEDESRKETNKKGT
  NEKQRRKRQAYESNLICHGLQLEATRSVLDDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKNRSSAF
  GTLNWFTKVLSVDESIIKPEQEFFTAPFEKNRMNDFYIVDRDAFFNPATRSRIVYFILSRVKYQVINNVS
  KFGINRLVNSGIYKAAFPLHDCKFRRQSEDPSCPNERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIY
  FAWLGYYTQMLLLAAVVGVACFLYGYLNQDNCTWSKEVCHPDIGGKIIMCPQCDRLCPFWKLNITCESSK
  KLCIFDSFGTLVFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQARPEYEARCTHVVINEITQEE
  ERIPFTAWGKCIRITLCASAVFFWILLIIASVIGIIVYRLSVFIVFSAKLPKNINGTDPIQKYLTPQTAT
  SITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVG
  YPGDPVYWLGKYRNEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWIMNLIGRFHRVSGSEKITPR
  WEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRLVPEKA
  QDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSVPPYGDHTSYTMEGYINNTLSIFKVADF
  KNKSKGNPYSDLGNHTTCRYRDFRYPPGHPQEYKHNIYYWHVIAAKLAFIIVMEHVIYSVKFFISYAIPD
  VSKRTKSKIQREKYLTQKLLHENHLKDMTKNMGVIAERMIEAVDNNLRPKSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142678
• ORF Size: 2676 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142678.1, NP_001136150.1
• RefSeq ORF: 2679
• Locus ID: 196527
• Protein Families: Transmembrane
• MW: 103.8 kDa
• Gene Summary: This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]