TRPC4 (NM_001135958) Human Tagged ORF Clone

CAT#: RC226733

TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPC4
• Synonyms: HTRP-4; HTRP4; TRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226733 representing NM_001135958
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA
  GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG
  TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA
  AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG
  TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA
  CCACAAAAAACCTAGTGGAGAAAAACAGTTTGTTGCCCAGCCCAATTGTCAACAGCTGCTGGCATCTCGC
  TGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGCAGTGAAGATGGTGACATGTTTCATAATAG
  GACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCTCCCAAAAGCCCACTTGGACTGTTCATCAG
  GAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATTTGACTTTTTTGTTCCTGCTGCTGCTTGCC
  TCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCCACCACCAACCATCGTCGAGTGGATGATAT
  TACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAGATGTGGGATGGCGGACTTCAGGACTACAT
  CCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCTTATATTTAGCAACAATCTCCTTGAAAATT
  GTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATCATGGGACATGTGGCATCCCACTCTGGTGG
  CAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTGCGTCTGATCTCACTGTTTACTGCAAATTC
  TCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCCTGGACATTTTGAAGTTTCTATTCATATAC
  TGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATTGTACTTCTATTATGAAGAAACGAAAGGGT
  TAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCATTTTCAACGTTATTTGAGACACTGCAGTC
  CCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGACCAATGTCAAAGCACAGCATGAATTTACT
  GAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCATCTCTCTGGTTGTTCTACTCAACATGTTAA
  TAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCATGCAGATATAGAATGGAAATTTGCACGAAC
  AAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGCCTACTCCCTTCAATGTCATCCCGAGCCCC
  AAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTTGTGCAAGAAAAAGATGAGAAGAAAGCCAG
  AAAGTTTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAAGTTAT
  GAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAA
  GAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAA
  GCAAACTTTCCACAATACAATCTGCGAATGCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAA
  GAGTGATAGCGAAGGTAATAGCAAGGACAAGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATT
  CATCCGAGATCAGCAGCAATTGCCTCTGAAAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGG
  AGCCGCCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAG
  ACGATCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGT
  CAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACC
  TGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGG
  GTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATA
  ATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACAG
  TCACCCACGAAGATTACGTGACCACAAGATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226733 representing NM_001135958
  Red=Cloning site Green=Tags(s)
  
  MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLG
  RTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQFVAQPNCQQLLASR
  WYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIAPKSPLGLFIRKPFIKFICHTASYLTFLFLLLLA
  SQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATISLKI
  VAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIY
  CLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNAFSTLFETLQSLFWSIFGLINLYVTNVKAQHEFT
  EFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSP
  KSLWYLIKWIWTHLCKKKMRRKPESFGTIGRRAADNLRRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTE
  ENFKELKQDISSFRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLI
  HPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRRSKQNAAEQNANQIFSVSEEVAR
  QQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVDHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPI
  IPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135958
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135958.1, NP_001129430.1
• RefSeq ORF: 2415 bp
• Locus ID: 7223
• Cytogenetics: 13q13.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 92 kDa
• Gene Summary: 'This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]'