TRPC4 (NM_001135958) Human Tagged ORF Clone
CAT#: RG226733
- TrueORF®
TRPC4 (GFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta
"NM_001135958" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | TRPC4 |
Synonyms | HTRP-4; HTRP4; TRP4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226733 representing NM_001135958
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA CCACAAAAAACCTAGTGGAGAAAAACAGTTTGTTGCCCAGCCCAATTGTCAACAGCTGCTGGCATCTCGC TGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGCAGTGAAGATGGTGACATGTTTCATAATAG GACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCTCCCAAAAGCCCACTTGGACTGTTCATCAG GAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATTTGACTTTTTTGTTCCTGCTGCTGCTTGCC TCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCCACCACCAACCATCGTCGAGTGGATGATAT TACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAGATGTGGGATGGCGGACTTCAGGACTACAT CCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCTTATATTTAGCAACAATCTCCTTGAAAATT GTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATCATGGGACATGTGGCATCCCACTCTGGTGG CAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTGCGTCTGATCTCACTGTTTACTGCAAATTC TCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCCTGGACATTTTGAAGTTTCTATTCATATAC TGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATTGTACTTCTATTATGAAGAAACGAAAGGGT TAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCATTTTCAACGTTATTTGAGACACTGCAGTC CCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGACCAATGTCAAAGCACAGCATGAATTTACT GAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCATCTCTCTGGTTGTTCTACTCAACATGTTAA TAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCATGCAGATATAGAATGGAAATTTGCACGAAC AAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGCCTACTCCCTTCAATGTCATCCCGAGCCCC AAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTTGTGCAAGAAAAAGATGAGAAGAAAGCCAG AAAGTTTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTGAGAAGACATCACCAATACCAAGAAGTTAT GAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAGATGCTAAAACTGAAGAAGGCCTGACCGAA GAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCGCTTTGAAGTCCTGGGATTACTAAGAGGAA GCAAACTTTCCACAATACAATCTGCGAATGCCTCGAAGGAGTCTTCAAATTCGGCAGACTCAGATGAAAA GAGTGATAGCGAAGGTAATAGCAAGGACAAGAAAAAGAATTTCAGCCTTTTTGATTTAACCACCCTGATT CATCCGAGATCAGCAGCAATTGCCTCTGAAAGACATAACATAAGCAATGGCTCTGCCCTGGTGGTTCAGG AGCCGCCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACCGATATCAAAAACTTTGGGTTATTTCATAG ACGATCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAATCTTCTCTGTTTCAGAAGAAGTTGCTCGT CAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGAATCTCGAGGATTAGCTTCACGGGGTGACC TGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGACCATAGAGAAAGGAATACGGACACACTGGG GTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGAAGGTGGTGGTGGAGGACACGGTTCCTATA ATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTATAGACTATGATCTAAACCTCCCAGACACAG TCACCCACGAAGATTACGTGACCACAAGATTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226733 representing NM_001135958
Red=Cloning site Green=Tags(s) MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLG RTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQFVAQPNCQQLLASR WYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIAPKSPLGLFIRKPFIKFICHTASYLTFLFLLLLA SQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATISLKI VAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIY CLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNAFSTLFETLQSLFWSIFGLINLYVTNVKAQHEFT EFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSP KSLWYLIKWIWTHLCKKKMRRKPESFGTIGRRAADNLRRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTE ENFKELKQDISSFRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLI HPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRRSKQNAAEQNANQIFSVSEEVAR QQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVDHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPI IPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001135958 |
ORF Size | 2412 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001135958.1, NP_001129430.1 |
RefSeq Size | 3015 bp |
RefSeq ORF | 2415 bp |
Locus ID | 7223 |
Cytogenetics | 13q13.3 |
Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
Gene Summary | 'This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC326235 | TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta |
USD 1,630.00 |
|
RC226733 | TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta |
USD 890.00 |
|
RC226733L3 | Lenti-ORF clone of TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta |
USD 1,090.00 |
|
RC226733L4 | Lenti-ORF clone of TRPC4 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta |
USD 1,090.00 |
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