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ANK1 (NM_001142446) Human Tagged ORF Clone
CAT#: RC227586
- TrueORF®
ANK1 (Myc-DDK-tagged)-Human ankyrin 1, erythrocytic (ANK1), transcript variant 9
"NM_001142446" in other vectors (2)
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Other products for "ANK1"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | ANK1 |
| Synonyms | ANK; SPH1; SPH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227586 representing NM_001142446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAAGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAGGAGCAGAAGG AGAAGGAGGAATCCAACAGGAAGCGGAGAAACCGCTCCCGTGACCGAAAGAAGAAGGCCGATGCTGCTAC CAGCTTTCTGAGAGCAGCAAGATCAGGTAACTTGGACAAAGCTTTGGATCACCTGCGGAATGGGGTAGAT ATTAACACCTGTAACCAGAATGGGTTGAATGGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATGG TGGTTGAACTTCTGCACAAAGAAATCATTCTAGAAACGACAACCAAGAAGGGGAACACGGCCCTGCACAT CGCTGCTCTAGCCGGGCAGGATGAGGTGGTCCGGGAGCTTGTCAACTATGGAGCCAACGTCAACGCCCAG TCACAGAAAGGTTTTACACCCCTGTACATGGCAGCACAAGAGAACCACTTGGAAGTGGTTAAGTTTTTAC TGGAAAATGGAGCTAACCAGAATGTAGCCACAGAAGACGGCTTCACGCCTCTGGCGGTAGCCCTGCAGCA GGGCCATGAGAACGTCGTCGCGCACCTCATCAACTACGGCACCAAGGGGAAGGTGCGCCTCCCGGCCCTG CACATCGCGGCCCGCAACGACGACACGCGCACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGACG TGCTTTCCAAGACGGGATTCACGCCCCTGCACATTGCGGCTCACTACGAGAACCTCAACGTGGCCCAGTT GCTCCTCAACAGAGGAGCCAGCGTCAATTTCACACCACAGAACGGCATCACGCCACTGCACATCGCCTCC CGCAGGGGCAACGTGATCATGGTGCGGCTGCTGCTGGATCGGGGAGCCCAGATAGAAACCAAGACCAAGG ACGAATTGACACCTCTCCACTGTGCAGCTCGAAATGGGCACGTGCGAATCTCAGAGATCCTGCTGGACCA CGGGGCACCAATCCAAGCCAAAACCAAGAACGGCCTGTCCCCAATTCACATGGCGGCTCAGGGAGACCAC CTCGACTGTGTCCGGCTCCTGTTGCAATACGACGCAGAGATAGACGACATCACCCTGGACCACCTGACCC CACTCCACGTGGCTGCCCACTGTGGACACCACAGGGTGGCTAAGGTCCTTCTGGATAAAGGGGCCAAACC CAACTCCAGAGCCCTGAATGGCTTTACCCCCTTACACATCGCCTGCAAAAAGAACCACGTCCGTGTCATG GAGCTGCTGCTGAAGACGGGAGCCTCGATCGACGCGGTCACCGAGTCTGGCCTGACACCTCTCCACGTGG CCTCCTTCATGGGGCACCTTCCCATCGTGAAGAACCTCCTGCAGCGGGGGGCGTCGCCCAACGTCTCCAA CGTGAAAGTGGAGACCCCGCTACACATGGCAGCCAGAGCCGGGCACACGGAAGTGGCCAAATATTTACTC CAGAACAAAGCCAAAGTCAATGCCAAGGCCAAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATCG GCCACACAAACATGGTGAAGCTCCTGCTGGAAAATAACGCCAACCCCAACCTGGCCACCACCGCCGGGCA CACCCCCCTGCACATTGCAGCCCGTGAGGGCCATGTGGAAACAGTCCTGGCCCTTCTGGAAAAGGAAGCA TCCCAGGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCGGCCAAGTACGGGAAGGTGCGGG TGGCAGAGCTGCTGCTGGAGCGGGACGCACACCCGAATGCTGCCGGAAAAAATGGCCTGACCCCCCTGCA CGTGGCCGTCCATCACAACAACCTGGACATCGTCAAGCTGCTGCTTCCCCGGGGCGGCTCCCCGCACAGC CCTGCCTGGAATGGCTACACCCCTTTGCACATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGTC TGCTGCAGTATGGGGGCTCAGCAAACGCCGAGTCGGTGCAAGGTGTGACGCCCCTTCACCTGGCCGCCCA GGAGGGCCACGCAGAGATGGTGGCTCTGCTGCTCTCGAAACAAGCCAATGGCAACCTGGGGAACAAGAGC GGACTCACTCCCCTCCATCTGGTAGCACAAGAAGGCCACGTTCCAGTGGCAGATGTGCTGATCAAACACG GCGTCATGGTGGACGCCACCACCCGGATGGGCTACACTCCCCTCCATGTGGCCAGTCACTATGGAAACAT CAAGCTGGTGAAGTTTCTGCTGCAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCC CTGCACCAGGCAGCCCAGCAGGGACACACAGACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCAA ACGAGGTCAGCTCGGATGGAACCACACCTCTGGCCATAGCCAAGCGCTTGGGCTACATTTCTGTCACCGA CGTGCTCAAGGTCGTCACGGATGAAACCAGTTTCGTGTTAGTCAGTGATAAGCATCGAATGAGTTTCCCT GAGACAGTTGATGAGATCCTGGATGTCTCGGAAGATGAAGGAACTGCTCATATAACTATAATGGGGGAAG AACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAAGAGAAGGAGCTGCTGGATTT TGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATCCCCAGGATTCCCTGTGCCATGCCTGAGACA GTGGTGATCAGGTCAGAAGAGCAGGAGCAGGCATCTAAAGAGTATGATGAGGACTCCCTCATCCCCAGCA GCCCGGCCACCGAGACCTCAGACAACATCAGCCCGGTGGCCAGCCCGGTGCATACAGGGTTTCTGGTGAG CTTCATGGTTGACGCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCCTGCGAGTGGTGATCCCG CCACGGACGTGCGCAGCGCCCACCCGCATCACCTGCCGCCTGGTCAAGCCCCAGAAGCTCAGCACGCCGC CCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGGCCCACGGGGGCACAGTTCCT GAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCATGGCCGTGGAGACCGCGAGCTCGTGGTTCTG AGGAGCGAAAACGGCTCCGTGTGGAAGGAGCACAGGAGCCGCTATGGAGAGAGCTACCTGGATCAGATCC TCAACGGGATGGACGAAGAGCTGGGGAGCCTGGAGGAGCTAGAGAAGAAGAGGGTGTGCCGAATCATCAC CACCGACTTCCCGCTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACGACACCATCGGTCCCGAA GGGGGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGAGAATGCCGTCACCAAGA GAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACTAAGCTCCTGGGCAACCAGGC CACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGGAAGTTCCACCGCCCCATTGGGCTTCGGATC CCACTACCTCCTTCCTGGACCGACAACCCGAGGGACAGCGGGGAGGGAGACACCACCAGCCTGCGCCTGC TTTGCAGCGTCATTGGAGGAACAGACCAAGCCCAGTGGGAAGACATAACAGGAACCACCAAACTTGTATA TGCCAACGAGTGCGCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGTCGGACTGTCCTCGGACT GCTGAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCCCTACATGGCCAAATTCG TCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTACTGCATGACAGATGATAAAGT GGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTGTTGGAA GGAATGTCCCTGTTTGCAGAACTCTCTGGGAACCTGGTGCCTGTGAAGAAAGCTGCCCAGCAGCGGAGCT TCCACTTCCAGTCATTTCGGGAGAACCGTCTGGCCATGCCTGTAAAGGTGAGGGACAGCAGTCGAGAGCC GGGAGGGTCCCTGTCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGCACATTCTCTGCCACCTG AACATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAGGACCCCGACGCCCCTGG CCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGGACAGAGCAGGCAGAGATGAA GATGGCTGTTATCTCAGAGCACCTCGGTCTCAGCTGGGCAGAGTTGGCCCGGGAGCTGCAGTTCAGTGTG GAAGACATCAACAGGATCCGAGTGGAAAATCCCAACTCCCTGTTGGAGCAGAGTGTGGCCTTGCTGAACC TCTGGGTCATCCGTGAAGGCCAAAACGCAAACATGGAGAATCTGTACACAGCCCTGCAGAGCATTGACCG TGGCGAGATCGTGAACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGAAGCCAGACAGGCGGCAC ACCGACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGTTACTCCTCACTGCAGGACGAGCTGCTGT CCCCTGCCTCCCTGGGCTGTGCACTTTCCTCTCCGCTACGTGCAGACCAGTACTGGAATGAGGTGGCCGT CCTAGACGCCATCCCCTTGGCGGCCACGGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGG TCTGCGGGCCTCACGCCTTCTCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGTAGCAAGGCTGAGGACT CTGATGCCACAGGTCACGAGTGGAAGTTGGAGGGGGCACTCTCAGAGGAACCGCGGGGCCCCGAGTTGGG CTCTCTGGAACTTGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTTATCGATTTGCTTGAA CAGGAGGAAGGTCAGAGGTCAGAAGAGAAGCTGCCAGGTTCTAAGAGGCAGGATGACGCGACAGGTGCAG GGCAGGACTCAGAGAATGAAGTGTCTCTTGTTTCAGGCCATCAGAGGGGGCAAGCCCGAATCACACATTC CCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGACTGCAGGACTGGGATGCAGACGGCTCGATT GTCTCATACCTGCAAGATGCTGCACAAGGTTCCTGGCAAGAGGAGGTCACGCAAGGTCCACACTCATTCC AGGGAACAAGTACCATGACTGAAGGGCTAGAGCCCGGTGGATCTCAGGAGTACGAGAAGGTCCTGGTGTC TGTAAGTGAGCACACGTGGACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCGACAGGGACCGGAGGCAG CAAGGCCAAGAAGAGCAGGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGTGCAGGGGAATGAGTTTC AGAATATTCCAGGGGAGCAGGTGACAGAGGAGCAATTCACGGATGAGCAGGGCAACATTGTCACCAAGAA GATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGCGCCGATGCCGCCCAGGAGCACGAGGAGGAT CACACCTCGACACCCAACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227586 representing NM_001142446
Red=Cloning site Green=Tags(s) MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVD INTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQ SQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPAL HIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIAS RRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDH LDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVM ELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL QNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEA SQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS PAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKS GLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSP LHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFP ETVDEILDVSEDEGTAHITIMGEELISFKAERRDSRDVDEEKELLDFVPKLDQVVESPAIPRIPCAMPET VVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIP PRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVL RSENGSVWKEHRSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPE GGSLKSKLVPLVQATFPENAVTKRVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRI PLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRT AEAVNFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLE GMSLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAMPVKVRDSSREPGGSLSFLRKAMKYEDTQHILCHL NITMPPCAKGSGAEDRRRTPTPLALRYSILSESTPGSLSGTEQAEMKMAVISEHLGLSWAELARELQFSV EDINRIRVENPNSLLEQSVALLNLWVIREGQNANMENLYTALQSIDRGEIVNMLEGSGRQSRNLKPDRRH TDRDYSLSPSQMNGYSSLQDELLSPASLGCALSSPLRADQYWNEVAVLDAIPLAATEHDTMLEMSDMQVW SAGLTPSLVTAEDSSLECSKAEDSDATGHEWKLEGALSEEPRGPELGSLELVEDDTVDSDATNGLIDLLE QEEGQRSEEKLPGSKRQDDATGAGQDSENEVSLVSGHQRGQARITHSPTVSQVTERSQDRLQDWDADGSI VSYLQDAAQGSWQEEVTQGPHSFQGTSTMTEGLEPGGSQEYEKVLVSVSEHTWTEQPEAESSQADRDRRQ QGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEED HTSTPNP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142446 |
| ORF Size | 5691 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142446.1, NP_001135918.1 |
| RefSeq ORF | 5694 bp |
| Locus ID | 286 |
| Cytogenetics | 8p11.21 |
| Protein Families | Transmembrane |
| MW | 208.1 kDa |
| Gene Summary | 'Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]' |
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