ANK1 (NM_001142446) Human Untagged Clone

CAT#: SC326408

ANK1 (untagged)-Human ankyrin 1, erythrocytic (ANK1), transcript variant 9

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANK1
• Synonyms: ANK; SPH1; SPH2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001142446, the custom clone sequence may differ by one or more nucleotides
  ATGGCTCAAGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAG
  GAGCAGAAGGAGAAGGAGGAATCCAACAGGAAGCGGAGAAACCGCTCCCGTGACCGAAAG
  AAGAAGGCCGATGCTGCTACCAGCTTTCTGAGAGCAGCAAGATCAGGTAACTTGGACAAA
  GCTTTGGATCACCTGCGGAATGGGGTAGATATTAACACCTGTAACCAGAATGGGTTGAAT
  GGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATGGTGGTTGAACTTCTGCACAAA
  GAAATCATTCTAGAAACGACAACCAAGAAGGGGAACACGGCCCTGCACATCGCTGCTCTA
  GCCGGGCAGGATGAGGTGGTCCGGGAGCTTGTCAACTATGGAGCCAACGTCAACGCCCAG
  TCACAGAAAGGTTTTACACCCCTGTACATGGCAGCACAAGAGAACCACTTGGAAGTGGTT
  AAGTTTTTACTGGAAAATGGAGCTAACCAGAATGTAGCCACAGAAGACGGCTTCACGCCT
  CTGGCGGTAGCCCTGCAGCAGGGCCATGAGAACGTCGTCGCGCACCTCATCAACTACGGC
  ACCAAGGGGAAGGTGCGCCTCCCGGCCCTGCACATCGCGGCCCGCAACGACGACACGCGC
  ACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGACGTGCTTTCCAAGACGGGATTC
  ACGCCCCTGCACATTGCGGCTCACTACGAGAACCTCAACGTGGCCCAGTTGCTCCTCAAC
  AGAGGAGCCAGCGTCAATTTCACACCACAGAACGGCATCACGCCACTGCACATCGCCTCC
  CGCAGGGGCAACGTGATCATGGTGCGGCTGCTGCTGGATCGGGGAGCCCAGATAGAAACC
  AAGACCAAGGACGAATTGACACCTCTCCACTGTGCAGCTCGAAATGGGCACGTGCGAATC
  TCAGAGATCCTGCTGGACCACGGGGCACCAATCCAAGCCAAAACCAAGAACGGCCTGTCC
  CCAATTCACATGGCGGCTCAGGGAGACCACCTCGACTGTGTCCGGCTCCTGTTGCAATAC
  GACGCAGAGATAGACGACATCACCCTGGACCACCTGACCCCACTCCACGTGGCTGCCCAC
  TGTGGACACCACAGGGTGGCTAAGGTCCTTCTGGATAAAGGGGCCAAACCCAACTCCAGA
  GCCCTGAATGGCTTTACCCCCTTACACATCGCCTGCAAAAAGAACCACGTCCGTGTCATG
  GAGCTGCTGCTGAAGACGGGAGCCTCGATCGACGCGGTCACCGAGTCTGGCCTGACACCT
  CTCCACGTGGCCTCCTTCATGGGGCACCTTCCCATCGTGAAGAACCTCCTGCAGCGGGGG
  GCGTCGCCCAACGTCTCCAACGTGAAAGTGGAGACCCCGCTACACATGGCAGCCAGAGCC
  GGGCACACGGAAGTGGCCAAATATTTACTCCAGAACAAAGCCAAAGTCAATGCCAAGGCC
  AAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATCGGCCACACAAACATGGTGAAG
  CTCCTGCTGGAAAATAACGCCAACCCCAACCTGGCCACCACCGCCGGGCACACCCCCCTG
  CACATTGCAGCCCGTGAGGGCCATGTGGAAACAGTCCTGGCCCTTCTGGAAAAGGAAGCA
  TCCCAGGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCGGCCAAGTACGGG
  AAGGTGCGGGTGGCAGAGCTGCTGCTGGAGCGGGACGCACACCCGAATGCTGCCGGAAAA
  AATGGCCTGACCCCCCTGCACGTGGCCGTCCATCACAACAACCTGGACATCGTCAAGCTG
  CTGCTTCCCCGGGGCGGCTCCCCGCACAGCCCTGCCTGGAATGGCTACACCCCTTTGCAC
  ATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGTCTGCTGCAGTATGGGGGCTCA
  GCAAACGCCGAGTCGGTGCAAGGTGTGACGCCCCTTCACCTGGCCGCCCAGGAGGGCCAC
  GCAGAGATGGTGGCTCTGCTGCTCTCGAAACAAGCCAATGGCAACCTGGGGAACAAGAGC
  GGACTCACTCCCCTCCATCTGGTAGCACAAGAAGGCCACGTTCCAGTGGCAGATGTGCTG
  ATCAAACACGGCGTCATGGTGGACGCCACCACCCGGATGGGCTACACTCCCCTCCATGTG
  GCCAGTCACTATGGAAACATCAAGCTGGTGAAGTTTCTGCTGCAGCACCAGGCAGATGTC
  AATGCCAAGACCAAGCTAGGATACAGCCCCCTGCACCAGGCAGCCCAGCAGGGACACACA
  GACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCAAACGAGGTCAGCTCGGATGGA
  ACCACACCTCTGGCCATAGCCAAGCGCTTGGGCTACATTTCTGTCACCGACGTGCTCAAG
  GTCGTCACGGATGAAACCAGTTTCGTGTTAGTCAGTGATAAGCATCGAATGAGTTTCCCT
  GAGACAGTTGATGAGATCCTGGATGTCTCGGAAGATGAAGGAACTGCTCATATAACTATA
  ATGGGGGAAGAACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAA
  GAGAAGGAGCTGCTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATC
  CCCAGGATTCCCTGTGCCATGCCTGAGACAGTGGTGATCAGGTCAGAAGAGCAGGAGCAG
  GCATCTAAAGAGTATGATGAGGACTCCCTCATCCCCAGCAGCCCGGCCACCGAGACCTCA
  GACAACATCAGCCCGGTGGCCAGCCCGGTGCATACAGGGTTTCTGGTGAGCTTCATGGTT
  GACGCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCCTGCGAGTGGTGATCCCG
  CCACGGACGTGCGCAGCGCCCACCCGCATCACCTGCCGCCTGGTCAAGCCCCAGAAGCTC
  AGCACGCCGCCCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGG
  CCCACGGGGGCACAGTTCCTGAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCAT
  GGCCGTGGAGACCGCGAGCTCGTGGTTCTGAGGAGCGAAAACGGCTCCGTGTGGAAGGAG
  CACAGGAGCCGCTATGGAGAGAGCTACCTGGATCAGATCCTCAACGGGATGGACGAAGAG
  CTGGGGAGCCTGGAGGAGCTAGAGAAGAAGAGGGTGTGCCGAATCATCACCACCGACTTC
  CCGCTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACGACACCATCGGTCCCGAA
  GGGGGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGAGAATGCC
  GTCACCAAGAGAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACT
  AAGCTCCTGGGCAACCAGGCCACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGG
  AAGTTCCACCGCCCCATTGGGCTTCGGATCCCACTACCTCCTTCCTGGACCGACAACCCG
  AGGGACAGCGGGGAGGGAGACACCACCAGCCTGCGCCTGCTTTGCAGCGTCATTGGAGGA
  ACAGACCAAGCCCAGTGGGAAGACATAACAGGAACCACCAAACTTGTATATGCCAACGAG
  TGCGCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGTCGGACTGTCCTCGGACT
  GCTGAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCCCTACATG
  GCCAAATTCGTCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTAC
  TGCATGACAGATGATAAAGTGGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTG
  GCCCGGAGCAGGGACATAGAGGTGTTGGAAGGAATGTCCCTGTTTGCAGAACTCTCTGGG
  AACCTGGTGCCTGTGAAGAAAGCTGCCCAGCAGCGGAGCTTCCACTTCCAGTCATTTCGG
  GAGAACCGTCTGGCCATGCCTGTAAAGGTGAGGGACAGCAGTCGAGAGCCGGGAGGGTCC
  CTGTCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGCACATTCTCTGCCACCTG
  AACATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAGGACCCCG
  ACGCCCCTGGCCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGG
  ACAGAGCAGGCAGAGATGAAGATGGCTGTTATCTCAGAGCACCTCGGTCTCAGCTGGGCA
  GAGTTGGCCCGGGAGCTGCAGTTCAGTGTGGAAGACATCAACAGGATCCGAGTGGAAAAT
  CCCAACTCCCTGTTGGAGCAGAGTGTGGCCTTGCTGAACCTCTGGGTCATCCGTGAAGGC
  CAAAACGCAAACATGGAGAATCTGTACACAGCCCTGCAGAGCATTGACCGTGGCGAGATC
  GTGAACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGAAGCCAGACAGGCGGCAC
  ACCGACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGTTACTCCTCACTGCAGGAC
  GAGCTGCTGTCCCCTGCCTCCCTGGGCTGTGCACTTTCCTCTCCGCTACGTGCAGACCAG
  TACTGGAATGAGGTGGCCGTCCTAGACGCCATCCCCTTGGCGGCCACGGAGCATGACACC
  ATGCTGGAGATGTCTGACATGCAGGTGTGGTCTGCGGGCCTCACGCCTTCTCTGGTCACT
  GCTGAGGACTCCTCTCTGGAGTGTAGCAAGGCTGAGGACTCTGATGCCACAGGTCACGAG
  TGGAAGTTGGAGGGGGCACTCTCAGAGGAACCGCGGGGCCCCGAGTTGGGCTCTCTGGAA
  CTTGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTTATCGATTTGCTTGAA
  CAGGAGGAAGGTCAGAGGTCAGAAGAGAAGCTGCCAGGTTCTAAGAGGCAGGATGACGCG
  ACAGGTGCAGGGCAGGACTCAGAGAATGAAGTGTCTCTTGTTTCAGGCCATCAGAGGGGG
  CAAGCCCGAATCACACATTCCCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGA
  CTGCAGGACTGGGATGCAGACGGCTCGATTGTCTCATACCTGCAAGATGCTGCACAAGGT
  TCCTGGCAAGAGGAGGTCACGCAAGGTCCACACTCATTCCAGGGAACAAGTACCATGACT
  GAAGGGCTAGAGCCCGGTGGATCTCAGGAGTACGAGAAGGTCCTGGTGTCTGTAAGTGAG
  CACACGTGGACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCGACAGGGACCGGAGGCAG
  CAAGGCCAAGAAGAGCAGGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGTGCAGGGG
  AATGAGTTTCAGAATATTCCAGGGGAGCAGGTGACAGAGGAGCAATTCACGGATGAGCAG
  GGCAACATTGTCACCAAGAAGATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGC
  GCCGATGCCGCCCAGGAGCACGAGGAGGATCACACCTCGACACCCAACCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001142446
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142446.1, NP_001135918.1
• RefSeq Size: 8486 bp
• RefSeq ORF: 5694 bp
• Locus ID: 286
• Cytogenetics: 8p11.21
• Protein Families: Transmembrane
• Gene Summary: 'Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (9) has an alternate upstream 5' exon, which includes an AUG start codon, and has an additional exon in the middle region, but lacks a segment in the 3' coding region, compared to variant 1. The reading frame is not affected and the resulting isoform (9) is the longest, which has a distinct N-terminus and an internal additional segment as well as lacks a segment in the C-terminal region, compared to isoform 1.