FLASH (CASP8AP2) (NM_001137668) Human Tagged ORF Clone

CAT#: RC227738

CASP8AP2 (Myc-DDK-tagged)-Human caspase 8 associated protein 2 (CASP8AP2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CASP8AP2
• Synonyms: CED-4; FLASH; RIP25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227738 representing NM_001137668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGATGATGACAATGGTGATGGAACAAGTTTATTTGATGTCTTTTCTGCTTCTCCTCTTAAGA
  ACAATGATGAAGGCTCACTGGACATATACGCTGGGTTGGACAGTGCTGTTTCTGACAGCGCTTCCAAATC
  CTGTGTACCATCAAGAAATTGTTTGGACTTATATGAAGAGATCCTGACTGAAGAAGGAACTGCAAAGGAG
  GCAACATATAATGATTTGCAAGTAGAATATGGAAAATGTCAACTACAAATGAAAGAGCTGATGAAAAAAT
  TTAAAGAAATACAGACACAGAATTTCAGCTTAATAAACGAAAACCAGTCTCTTAAGAAGAATATTTCAGC
  ACTTATCAAAACTGCCAGAGTGGAAATAAACCGCAAGGATGAAGAAATAAGTAATCTTCACCAAAGATTG
  TCTGAGTTTCCACATTTTCGAAATAATCATAAAACTGCAAGGACATTTGATACAGTTAAAACAAAAGATC
  TTAAATCTAGATCTCCACATTTGGATGATTGTTCAAAGACTGATCACAGAGCTAAAAGTGATGTTTCTAA
  AGATGTACATCATAGCACTTCACTGCCAAATCTGGAAAAGGAAGGAAAACCACATTCTGATAAAAGGAGT
  ACTTCACATTTACCTACATCTGTTGAGAAACACTGCACTAATGGTGTTTGGTCACGTTCTCATTATCAGG
  TTGGCGAGGGTAGCTCAAATGAGGATAGTAGAAGAGGAAGAAAAGATATTAGACATAGCCAGTTTAACAG
  AGGAACTGAAAGAGTACGAAAAGACTTAAGTACTGGCTGTGGTGATGGTGAACCAAGGATATTGGAGGCT
  AGTCAAAGGCTACAAGGACATCCTGAGAAATATGGTAAAGGTGAACCAAAGACTGAAAGCAAAAGTTCGA
  AGTTTAAAAGTAACTCAGATTCTGACTATAAAGGTGAACGCATTAACTCTTCTTGGGAGAAAGAGACCCC
  TGGAGAAAGGTCACACAGTCGAGTAGACTCTCAAAGTGACAAAAAACTAGAAAGACAAAGTGAAAGATCA
  CAAAATATAAATAGGAAAGAAGTTAAATCACAAGACAAAGAAGAAAGAAAAGTTGATCAAAAACCTAAAT
  CAGTAGTAAAGGACCAAGATCACTGGAGAAGATCTGAACGAGCATCACTTCCTCATTCCAAGAATGAAAT
  AACATTTTCTCATAATTCAAGTAAATACCATCTAGAAGAGAGAAGAGGATGGGAAGATTGTAAAAGAGAC
  AAGAGTGTAAACAGTCATAGTTTTCAAGATGGAAGATGTCCATCTTCTCTTTCAAACAGTAGAACTCACA
  AAAACATTGACTCTAAGGAAGTTGATGCCATGCATCAGTGGGAAAATACACCTTTAAAAGCAGAAAGACA
  TAGAACTGAAGATAAGAGGAAAAGAGAACAAGAAAGCAAAGAAGAAAATAGGCATATTAGAAATGAAAAA
  AGAGTACCTACAGAACATTTGCAGAAGACTAATAAGGAAACTAAGAAAACCACTACTGATTTAAAGAAAC
  AGAATGAACCAAAGACTGATAAGGGAGAAGTCCTTGATAATGGTGTTTCTGAAGGAGCAGATAATAAAGA
  GCTTGCAATGAAAGCTGAGAGTGGTCCAAATGAAACAAAAAACAAGGACCTAAAATTGAGTTTTATGAAA
  AAATTGAACTTAACTCTTTCTCCTGCTAAAAAGCAACCTGTTTCCCAGGATAATCAGCATAAAATAACTG
  ATATTCCCAAGTCCAGTGGTGTATGTGATTCAGAGTCTTCAATGCAAGTTAAAACAGTGGCATATGTTCC
  CTCCATAAGTGAACATATCTTGGGGGAAGCAGCTGTCAGTGAACATACCATGGGGGAAACCAAGTCAACG
  TTATTGGAACCAAAGGTTGCTCTTCTAGCAGTGACTGAACCCAGGATCGGTATCTCAGAAACCAACAAGG
  AAGACGAAAATAGTTTGTTAGTTAGGTCTGTTGACAATACTATGCATTGTGAAGAGCCCATTTGTGGTAC
  AGAGACTTCCTTCCCATCTCCTATGGAAATACAACAGACAGAATCCTTGTTTCCATCAACAGGAATGAAA
  CAAACCATTAATAATGGAAGGGCAGCAGCTCCTGTGGTAATGGATGTATTACAAACAGATGTGTCTCAAA
  ACTTTGGCTTGGAATTGGATACCAAAAGAAATGATAATTCAGATTATTGTGGTATTTCTGAAGGTATGGA
  GATGAAGGTGGCACTTTCAACAACAGTGAGTGAAACCACTGAAAGCATTTTGCAGCCTTCAATTGAGGAA
  GCTGATATTTTGCCAATAATGCTTTCAGAAGATAATAACCCAAAATTTGAGCCTTCTGTTATAGTTACAC
  CACTGGTTGAGAGTAAGTCGTGTCATCTGGAGCCTTGCTTACCTAAAGAGACTCTAGATTCTTCACTTCA
  GCAGACTGAGTTAATGGACCACAGAATGGCAACTGGTGAAACAAACTCAGTATATCATGATGATGATAAC
  TCGGTTTTGAGCATTGACCTTAATCACCTGAGACCTATTCCAGAAGCCATCAGTCCTCTGAATAGTCCAG
  TGAGACCTGTAGCAAAAGTTCTTAGAAATGAAAGCCCACCTCAAGTTCCAGTGTATAATAACAGTCATAA
  AGATGTGTTTTTACCAAATTCAGCTCATTCTACCTCTAAGAGTCAGTCTGATCTCAATAAGGAAAATCAA
  AAGCCAATTTACAAATCTGACAAATGTACAGAAGCAGACACATGTAAGAATTCACCATTAGATGAATTAG
  AAGAAGGAGAAATTAGAAGTGATAGTGAAACATCTAAACCACAAGAAAGTTTTGAAAAAAATTCCAAACG
  TAGAGTGTCAGCTGATGTGCGGAAGTCAAAGACTATCCCACGACGTGGGAAAAGTACTGTGTGTTTAGAT
  AAAGACAGTAGGAAAACACATGTAAGAATCCATCAGACCAATAACAAATGGAATAAAAGACCTGATAAAT
  CTAGCAGATCTTCAAAAACAGAGAAGAAAGATAAAGTGATGAGCACTTCCAGCTTGGAAAAAATAGTTCC
  AATTATTGCTGTACCCTCTTCTGAACAAGAGATCATGCACATGTTACGAATGATAAGAAAACATGTAAGA
  AAAAATTATATGAAATTCAAGGCAAAATTTTCATTAATACAATTTCACAGAATTATTGAGTCAGCAATTT
  TGAGTTTTACATCTTTAATTAAACATCTCAACTTACACAAAATCTCTAAGTCAGTGACTACCTTACAGAA
  GAATCTCTGTGATATTATAGAGTCTAAACTTAAGCAAGTTAAAAAGAATGGCATAGTTGATCGTTTATTT
  GAACAGCAACTACCAGATATGAAAAAAAAATTGTGGAAGTTTGTAGATGACCAACTTGATTATTTGTTTG
  CAAAGCTTAAGAAAATCTTAGTATGTGATTCCAAAAGCTTTGGAAGAGATAGTGATGAAGGCAAACTTGA
  AAAAACAAGTAAACAGAATGCACAGTATTCAAATAGTCAGAAAAGGAGTGTGGACAACTCCAACAGAGAA
  TTGCTGAAAGAAAAATTATCAAAATCAGAAGACCCTGTTCATTATAAGTCTTTAGTGGGATGTAAAAAAT
  CTGAGGAAAATTATCAAGACCAAAATAACTCCAGTATTAACACTGTAAAGCATGACATTAAAAAAAATTT
  TAACATCTGCTTTGATAATATAAAGAACTCTCAATCCGAAGAGCGCTCCTTGGAAGTACACTGTCCAAGC
  ACCCCAAAGTCAGAAAAAAACGAAGGAAGCAGCATAGAGGATGCACAGACATCCCAGCATGCAACTTTGA
  AGCCAGAACGAAGTTTCGAGATTCTTACCGAACAGCAAGCATCGAGCCTTACTTTTAATTTAGTGAGTGA
  TGCACAAATGGGTGAAATATTTAAAAGTTTGTTGCAAGGTTCTGATCTTTTAGACAGCAGTGTTAACTGT
  ACTGAAAAAAGTGAGTGGGAGTTAAAGACTCCAGAGAAGCAGCTGCTAGAGACTCTTAAGTGCGAGTCTA
  TACCAGCTTGTACAACAGAAGAGCTAGTTTCAGGGGTGGCTTCTCCATGTCCTAAAATGATTAGTGATGA
  TAATTGGTCATTATTATCATCTGAAAAAGGTCCATCTCTGTCTTCAGGGCTTTCATTGCCGGTTCATCCT
  GATGTGTTGGATGAAAGTTGTATGTTTGAAGTGTCTACTAACCTACCTTTAAGTAAAGATAATGTGTGTA
  GTGTAGAAAAGAGCAAGCCCTGCGTTTCTTCCATACTTCTTGAAGATCTAGCAGTCTCTTTAACAGTACC
  ATCGCCTCTGAAGTCAGATGGTCATCTCAGTTTTTTAAAGCCTGATATGTCGTCCAGTTCAACTCCTGAA
  GAAGTCATTAGTGCTCATTTTAGTGAAGATGCCTTACTTGAGGAAGAGGATGCATCTGAGCAAGATATTC
  ATTTAGCTCTGGAGTCTGATAATTCAAGCAGTAAATCAAGTTGTTCTTCTTCCTGGACAAGCCGATCTGT
  TGCTCCAGGCTTTCAGTACCACCCTAATCTACCTATGCATGCCGTCATAATGGAAAAGTCCAATGATCAT
  TTCATTGTGAAAATACGACGTGCAACACCATCTACCTCTTCTGGCCTTAAACAGAGTATGATGCCTGATG
  AATTATTGACATCTTTGCCCAGACATGGAAAGGAAGCTGATGAAGGACCAGAGAAAGAATATATTTCATG
  TCAGAACACAGTTTTTAAATCTGTGGAGGAATTGGAAAACTCCAACAAAAATGTTGATGGCAGCAAGTCA
  ACTCATGAAGAACAGAGCTCTATGATACAAACACAGGTTCCTGATATATATGAATTTCTTAAAGATGCTT
  CAGATAAGATGGGTCATAGTGATGAAGTGGCTGATGAATGTTTCAAATTGCATCAAGTATGGGAAACAAA
  AGTGCCTGAAAGCATTGAAGAATTGCCTTCAATGGAAGAAATCTCACACTCTGTTGGGGAACATCTTCCA
  AACACATACGTAGATCTAACGAAAGATCCAGTCACTGAAACCAAAAACTTGGGGGAATTCATAGAAGTAA
  CAGTTTTACATATTGATCAGTTGGGATGTTCTGGAGGCAATTTAAATCAGAGTGCTCAAATATTAGACAA
  TTCTTTGCAGGCTGATACTGTAGGTGCTTTTATTGATTTGACACAAGATGCTTCAAGTGAGGCTAAAAGT
  GAAGGTAATCATCCTGCATTAGCTGTGGAAGACTTGGGATGTGGGGTGATACAGGTAGATGAAGATAATT
  GTAAGGAAGAAAAGGCACAAGTGGCAAACAGGCCTTTAAAATGCATTGTTGAGGAAACCTATATCGACTT
  GACCACAGAATCTCCCAGTTCATGTGAAGTAAAAAAAGATGAGTTAAAATCAGAGCCAGGATCAAATTGT
  GATAACTCGGAGTTGCCTGGGACTTTGCATAATTCTCACAAAAAGAGAAGAAACATTTCTGATCTAAATC
  ATCCTCATAAAAAACAAAGAAAGGAAACAGACTTAACTAATAAGGAAAAGACCAAGAAACCTACCCAAGA
  TTCTTGTGAGAATACTGAAGCTCACCAAAAGAAAGCCAGTAAGAAGAAGGCCCCTCCTGTGACTAAAGAT
  CCCTCATCATTAAAGGCAACCCCAGGGATTAAGGATTCATCAGCAGCACTTGCCACTTCTACAAGTCTTT
  CTGCAAAAAATGTTATTAAAAAGAAGGGAGAAATTATCATTTTATGGACAAGAAATGATGACCGGGAAAT
  TTTATTGGAGTGTCAGAAAAGAGGGCCATCATTTAAAACATTTGCATATTTAGCCGCCAAGTTGGATAAA
  AATCCAAATCAGGTCTCAGAAAGATTCCAGCAGCTAATGAAGCTCTTTGAAAAGTCAAAATGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227738 representing NM_001137668
  Red=Cloning site Green=Tags(s)
  
  MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKE
  ATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRL
  SEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRS
  TSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEA
  SQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERS
  QNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRD
  KSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEK
  RVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMK
  KLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKST
  LLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMK
  QTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEE
  ADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDN
  SVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQ
  KPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLD
  KDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVR
  KNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLF
  EQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRE
  LLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPS
  TPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNC
  TEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHP
  DVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPE
  EVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDH
  FIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKS
  THEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLP
  NTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKS
  EGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNC
  DNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKD
  PSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDK
  NPNQVSERFQQLMKLFEKSKCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001137668
• ORF Size: 5946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001137668.1, NP_001131140.1
• RefSeq ORF: 5949
• Locus ID: 9994
• Protein Families: Druggable Genome
• MW: 222.5 kDa
• Gene Summary: This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Nov 2008]