ATF6 beta (ATF6B) (NM_001136153) Human Tagged ORF Clone

CAT#: RC227970

ATF6B (Myc-DDK-tagged)-Human activating transcription factor 6 beta (ATF6B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATF6B
• Synonyms: CREB-RP; CREBL1; G13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227970 representing NM_001136153
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGATGCTGCTCAGCGAGATTGCTGACCCGACGCGTTTCTTCACCGACAACCTGCTTAGCC
  CGGAGGACTGGGACAGCACCTTGTATTCTGGCCTAGATGAAGTGGCCGAGGAGCAGACGCAGCTCTTCCG
  TTGCCCGGAGCAGGATGTCCCGTTTGACGGCAGCTCCCTGGACGTGGGGATGGATGTCAGCCCCTCTGAG
  CCCCCATGGGAACTCCTGCCGATCTTCCCAGATCTTCAGGTGAAGTCTGAGCCATCTTCCCCCTGCTCTT
  CCTCCTCCCTCAGCTCCGAGTCATCGCGTCTCTCCACAGAGCCATCCAGCGAGGCTCTTGGGGTAGGGGA
  GGTGCTCCATGTGAAGACAGAGTCCTTGGCACCCCCACTGTGTCTCCTGGGAGATGACCCAACATCCTCA
  TTTGAAACCGTCCAGATCAACGTTATCCCCACCTCTGATGATTCCTCAGATGTCCAGACCAAGATAGAAC
  CTGTCTCTCCATGTTCTTCCGTCAACTCTGAGGCCTCCCTGCTCTCAGCCGACTCCTCCAGCCAGGCTTT
  TATAGGAGAGGAGGTCCTGGAAGTGAAGACAGAGTCCCTGTCCCCTTCAGGATGCCTCCTGTGGGATGTC
  CCAGCCCCCTCACTTGGAGCTGTCCAGATCAGCATGGGCCCATCCCTTGATGGCTCCTCAGGCAAAGCCC
  TGCCCACCCGGAAGCCGCCACTGCAGCCCAAACCTGTAGTGCTAACCACTGTCCCAATGCCATCCAGAGC
  TGTGCCTCCCAGCACCACAGTCCTTCTGCAGTCCCTCGTCCAGCCACCCCCAGTGTCCCCAGTTGTCCTC
  ATCCAGGGTGCTATTCGAGTCCAGCCTGAAGGGCCGGCTCCCTCTCTACCACGGCCTGAGAGGAAGAGCA
  TCGTTCCCGCTCCTATGCCTGGAAACTCCTGCCCGCCTGAAGTGGATGCAAAGCTGCTGAAGCGGCAGCA
  GCGAATGATCAAGAACCGGGAGTCAGCCTGCCAGTCCCGGAGAAAGAAGAAAGAGTATCTGCAGGGACTG
  GAGGCTCGGCTGCAAGCAGTACTGGCTGACAACCAGCAGCTCCGCCGAGAGAATGCTGCCCTCCGGCGGC
  GGCTGGAGGCCCTGCTGGCTGAAAACAGCGAGCTCAAGTTAGGGTCTGGAAACAGGAAGGTGGTCTGCAT
  CATGGTCTTCCTTCTCTTCATTGCCTTCAACTTTGGACCTGTCAGCATCAGTGAGCCTCCTTCAGCTCCC
  ATCTCTCCTCGGATGAACAAGGGGGAGCCTCAACCCCGGAGACACTTGCTGGGGTTCTCAGAGCAAGAGC
  CAGTTCAGGGAGTTGAACCTCTCCAGGGGTCCTCCCAGGGCCCTAAGGAGCCCCAGCCCAGCCCCACAGA
  CCAGCCCAGTTTCAGCAACCTGACAGCCTTCCCTGGGGGCGCCAAGGAGCTACTACTAAGAGACCTAGAC
  CAGCTCTTCCTCTCCTCTGATTGCCGGCACTTCAACCGCACTGAGTCCCTGAGGCTTGCTGACGAGTTGA
  GTGGCTGGGTCCAGCGCCACCAGAGAGGCCGGAGGAAGATCCCTCAGAGGGCCCAGGAGAGACAGAAGTC
  TCAGCCACGGAAGAAGTCACCTCCAGTTAAGGCAGTCCCCATCCAACCCCCTGGACCCCCAGAAAGGGAT
  TCTGTGGGCCAGCTGCAACTATATCGCCACCCAGACCGTTCGCAGCCAGCATTCTTGGATGCAATTGACC
  GACGGGAAGACACATTTTATGTTGTCTCTTTCCGAAGGGACCACCTGCTGCTCCCAGCCATCAGCCACAA
  CAAGACCTCCCGGCCCAAGATGTCCCTGGTGATGCCTGCCATGGCCCCCAATGAGACCCTGTCAGGCCGT
  GGGGCCCCGGGGGACTATGAGGAGATGATGCAGATCGAGTGTGAGGTCATGGACACCAGGGTGATTCACA
  TCAAGACCTCCACAGTGCCCCCCTCGCTCCGAAAACAGCCATCCCCAACCCCAGGCAATGCCACAGGTGG
  CCCCTTGCCAGTCTCTGCAGCCAGCCAGGCCCACCAGGCCTCCCACCAGCCCCTCTACCTCAATCATCCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227970 representing NM_001136153
  Red=Cloning site Green=Tags(s)
  
  MAELMLLSEIADPTRFFTDNLLSPEDWDSTLYSGLDEVAEEQTQLFRCPEQDVPFDGSSLDVGMDVSPSE
  PPWELLPIFPDLQVKSEPSSPCSSSSLSSESSRLSTEPSSEALGVGEVLHVKTESLAPPLCLLGDDPTSS
  FETVQINVIPTSDDSSDVQTKIEPVSPCSSVNSEASLLSADSSSQAFIGEEVLEVKTESLSPSGCLLWDV
  PAPSLGAVQISMGPSLDGSSGKALPTRKPPLQPKPVVLTTVPMPSRAVPPSTTVLLQSLVQPPPVSPVVL
  IQGAIRVQPEGPAPSLPRPERKSIVPAPMPGNSCPPEVDAKLLKRQQRMIKNRESACQSRRKKKEYLQGL
  EARLQAVLADNQQLRRENAALRRRLEALLAENSELKLGSGNRKVVCIMVFLLFIAFNFGPVSISEPPSAP
  ISPRMNKGEPQPRRHLLGFSEQEPVQGVEPLQGSSQGPKEPQPSPTDQPSFSNLTAFPGGAKELLLRDLD
  QLFLSSDCRHFNRTESLRLADELSGWVQRHQRGRRKIPQRAQERQKSQPRKKSPPVKAVPIQPPGPPERD
  SVGQLQLYRHPDRSQPAFLDAIDRREDTFYVVSFRRDHLLLPAISHNKTSRPKMSLVMPAMAPNETLSGR
  GAPGDYEEMMQIECEVMDTRVIHIKTSTVPPSLRKQPSPTPGNATGGPLPVSAASQAHQASHQPLYLNHP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136153
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136153.1, NP_001129625.1
• RefSeq ORF: 2103 bp
• Locus ID: 1388
• Cytogenetics: 6p21.32
• Protein Families: Transcription Factors
• MW: 76.2 kDa
• Gene Summary: 'The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]'