hnRNP Q (SYNCRIP) (NM_001159675) Human Tagged ORF Clone

CAT#: RC228476

SYNCRIP (Myc-DDK-tagged)-Human synaptotagmin binding, cytoplasmic RNA interacting protein (SYNCRIP), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY431504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228476 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYNCRIP
• Synonyms: GRY-RBP; GRYRBP; hnRNP-Q; HNRNPQ; HNRPQ1; NSAP1; PP68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228476 representing NM_001159675
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAACATGTTAATGGAAATGGTACTGAAGAGCCCATGGATACTACTTCTGCAGTTATCCATT
  CAGAAAATTTTCAGACATTGCTTGATGCTGGTTTACCACAGAAAGTTGCTGAAAAACTAGATGAAATTTA
  CGTTGCAGGGCTAGTTGCACATAGTGATTTAGATGAAAGAGCTATTGAAGCTTTAAAAGAATTCAATGAA
  GACGGTGCATTGGCAGTTCTTCAACAGTTTAAAGACAGTGATCTCTCTCATGTTCAGAACAAAAGTGCCT
  TTTTATGTGGAGTCATGAAGACTTACAGGCAGAGAGAAAAACAAGGGACCAAAGTAGCAGATTCTAGTAA
  AGGACCAGATGAGGCAAAAATTAAGGCACTCTTGGAAAGAACAGGCTACACACTTGATGTGACCACTGGA
  CAGAGGAAGTATGGAGGACCACCTCCAGATTCCGTTTATTCAGGTCAGCAGCCTTCTGTTGGCACTGAGA
  TATTTGTGGGAAAGATCCCAAGAGATCTATTTGAGGATGAACTTGTTCCATTATTTGAGAAAGCTGGACC
  TATATGGGATCTTCGTCTAATGATGGATCCACTCACTGGTCTCAATAGAGGTTATGCGTTTGTCACTTTT
  TGTACAAAAGAAGCAGCTCAGGAGGCTGTTAAACTGTATAATAATCATGAAATTCGTTCTGGAAAACATA
  TTGGTGTCTGCATCTCAGTTGCCAACAATAGGCTTTTTGTGGGCTCTATTCCTAAGAGTAAAACCAAGGA
  ACAGATTCTTGAAGAATTTAGCAAAGTAACAGAGGGTCTTACAGACGTCATTTTATACCACCAACCGGAT
  GACAAGAAAAAAAACAGAGGCTTTTGCTTTCTTGAATATGAAGATCACAAAACAGCTGCCCAGGTAAAAG
  TGCTGTTTGTACGCAACCTTGCCAATACTGTAACAGAAGAGATTTTAGAAAAGGCATTTAGTCAGTTTGG
  GAAACTGGAACGAGTGAAGAAGTTAAAAGATTATGCGTTCATTCATTTTGATGAGCGAGATGGTGCTGTC
  AAGGCTATGGAAGAAATGAATGGCAAAGACTTGGAGGGAGAAAATATTGAAATTGTTTTTGCCAAGCCAC
  CAGATCAGAAAAGGAAAGAAAGAAAAGCTCAGAGGCAAGCAGCAAAAAATCAAATGTATGACGATTACTA
  CTATTATGGTCCACCTCATATGCCCCCTCCAACAAGAGGTCGAGGGCGTGGAGGTAGAGGTGGTTATGGA
  TATCCTCCAGATTATTATGGATATGAAGATTATTATGATTATTATGGTTATGATTACCATAACTATCGTG
  GTGGATATGAAGATCCATACTATGGTTATGAAGATTTTCAAGTTGGAGCTAGAGGAAGGGGTGGTAGAGG
  AGCAAGGGGTGCTGCTCCATCCAGAGGTCGTGGGGCTGCTCCTCCCCGCGGTAGAGCCGGTTATTCACAG
  AGAGGAGGTCCTGGATCAGCAAGAGGCGTTCGAGGTGCGAGAGGAGGTGCCCAACAACAAAGAGGCCGCG
  GGGTACGTGGTGCGAGGGGTGGCCGCGGTGGAAATGTAGGAGGAAAGCGCAAAGCTGATGGGTACAACCA
  GCCAGATTCCAAGCGGCGCCAGACCAATAATCAGAACTGGGGCTCCCAACCCATTGCTCAGCAACCGCTC
  CAAGGTGGTGATCATTCTGGTAACTATGGTTACAAATCTGAAAACCAGGAGTTTTATCAGGATACTTTTG
  GGCAACAGTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228476 representing NM_001159675
  Red=Cloning site Green=Tags(s)
  
  MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNE
  DGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTG
  QRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTF
  CTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPD
  DKKKNRGFCFLEYEDHKTAAQVKVLFVRNLANTVTEEILEKAFSQFGKLERVKKLKDYAFIHFDERDGAV
  KAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYG
  YPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQ
  RGGPGSARGVRGARGGAQQQRGRGVRGARGGRGGNVGGKRKADGYNQPDSKRRQTNNQNWGSQPIAQQPL
  QGGDHSGNYGYKSENQEFYQDTFGQQWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159675
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159675.1, NP_001153147.1
• RefSeq ORF: 1767
• Locus ID: 10492
• Protein Families: Stem cell - Pluripotency
• MW: 65.5 kDa
• Gene Summary: This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20. [provided by RefSeq, Dec 2011]