STAU2 (NM_001164384) Human Tagged ORF Clone

CAT#: RC228925

STAU2 (Myc-DDK-tagged)-Human staufen, RNA binding protein, homolog 2 (Drosophila) (STAU2), transcript variant 6

Product Images

Western blot validation of overexpression lysate (Cat# LY431953) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228925 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAU2
• Synonyms: 39K2; 39K3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228925 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCAAATAAATCAGATGTTCTCAGTGCAGCTGAGTCTTGGTGAGCAGACATGGGAATCCGAAGGCA
  GCAGTATAAAGAAGGCTCAGCAGGCTGTTGCCAATAAAGCTTTGACTGAATCTACGCTTCCCAAACCAGT
  TCAGAAGCCACCCAAAAGTAATGTTAACAATAACCCAGGCAGTATAACTCCAACTGTGGAACTGAATGGG
  CTTGCTATGAAAAGGGGAGAGCCTGCCATCTACAGGCCATTAGATCCAAAGCCATTCCCAAATTATAGAG
  CTAATTACAACTTTCGGGGCATGTACAATCAGAGGTATCATTGCCCAGTGCCTAAGATCTTTTATGTTCA
  GCTCACTGTAGGAAATAATGAATTTTTTGGGGAAGGAAAGACTCGACAAGCTGCTAGACACAATGCTGCA
  ATGAAAGCCCTCCAAGCACTGCAGAATGAACCTATTCCAGAAAGATCTCCTCAGAATGGTGAATCAGGAA
  AGGATATGGATGATGACAAAGATGCAAATAAGTCTGAGATCAGCTTAGTGTTTGAAATTGCTCTGAAGCG
  AAATATGCCTGTCAGTTTTGAGGTTATTAAAGAAAGTGGACCACCACATATGAAAAGCTTTGTTACTCGA
  GTGTCAGTAGGAGAGTTCTCTGCAGAAGGAGAAGGAAATAGCAAAAAACTCTCCAAGAAGCGCGCTGCGA
  CCACCGTCTTACAGGAGCTTAAAAAACTTCCACCTCTTCCTGTGGTGGAAAAGCCAAAACTATTTTTTAA
  AAAACGCCCTAAAACAATAGTAAAGGCCGGACCAGAATATGGCCAAGGGATGAACCCTATTAGCCGCCTG
  GCGCAAATTCAACAGGCCAAAAAGGAAAAGGAGCCGGATTATGTTTTGCTTTCAGAAAGAGGAATGCCTC
  GACGTCGAGAATTTGTGATGCAGGTGAAGGTAGGCAATGAAGTTGCTACAGGAACAGGACCTAATAAAAA
  GATAGCCAAAAAAAATGCTGCAGAAGCAATGCTGTTACAACTTGGTTATAAAGCATCCACTAATCTTCAG
  GATCAACTTGAGAAGACAGGGGAAAACAAAGGATGGAGTGGTCCAAAGCCTGGGTTTCCTGAACCAACAA
  ATAATACTCCAAAAGGAATTCTTCATTTGTCTCCTGATGTTTATCAAGAGATGGAAGCCAGCCGCCACAA
  AGTAATCTCTGGCACTACTCTAGGCTATTTGTCACCCAAAGATATGAACCAACCTTCAAGCTCTTTCTTC
  AGTATATCTCCCACATCGAATAGTTCAGCTACAATTGCCAGGGAACTCCTTATGAATGGAACATCTTCTA
  CAGCTGAAGCCATAGGTTTAAAAGGAAGTTCTCCTACTCCCCCTTGTTCTCCAGTACAACCTTCAAAACA
  ACTGGAATATTTAGCAAGGATTCAAGGCTTTCAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228925 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQINQMFSVQLSLGEQTWESEGSSIKKAQQAVANKALTESTLPKPVQKPPKSNVNNNPGSITPTVELNG
  LAMKRGEPAIYRPLDPKPFPNYRANYNFRGMYNQRYHCPVPKIFYVQLTVGNNEFFGEGKTRQAARHNAA
  MKALQALQNEPIPERSPQNGESGKDMDDDKDANKSEISLVFEIALKRNMPVSFEVIKESGPPHMKSFVTR
  VSVGEFSAEGEGNSKKLSKKRAATTVLQELKKLPPLPVVEKPKLFFKKRPKTIVKAGPEYGQGMNPISRL
  AQIQQAKKEKEPDYVLLSERGMPRRREFVMQVKVGNEVATGTGPNKKIAKKNAAEAMLLQLGYKASTNLQ
  DQLEKTGENKGWSGPKPGFPEPTNNTPKGILHLSPDVYQEMEASRHKVISGTTLGYLSPKDMNQPSSSFF
  SISPTSNSSATIARELLMNGTSSTAEAIGLKGSSPTPPCSPVQPSKQLEYLARIQGFQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164384
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164384.1, NP_001157856.1
• RefSeq Size: 4225 bp
• RefSeq ORF: 1440 bp
• Locus ID: 27067
• Protein Families: Transcription Factors
• MW: 52.8 kDa
• Gene Summary: Staufen homolog 2 is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. Staufen homolog 2 shares 48.5% and 59.9% similarity with drosophila and human staufen, respectively. The exact function of Staufen homolog 2 is not known, but since it contains 3 copies of conserved dsRNA binding domain, it could be involved in double-stranded RNA binding events. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]