SAP1 (PTPRH) (NM_001161440) Human Tagged ORF Clone
CAT#: RC229043
- TrueORF®
PTPRH (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, H (PTPRH), transcript variant 2
"NM_001161440" in other vectors (2)
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Other products for "PTPRH"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PTPRH |
Synonyms | R-PTP-H; SAP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC229043 representing NM_001161440
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGCTGGCGGGGGCCTCGGGGTCTGGGGGAACCTGGTGCTGCTGGGCCTGTGCAGCTGGACAG GGGCCAGGGCGCCTGCCCCCAACCCAGGGAGGAACCTGACAGTGGAGACTCAGACCACCAGCTCCATCTC CCTGAGCTGGGAGGTCCCCGATGGCCTAGACTCACAGAACTCCAACTACTGGGTTCAGTGTACTGGAGAC GGCGGCACAACAGAGACTCGAAACACAACAGCCACCAACGTCACCGTGGATGGCCTTGGACCCGGGTCAT TGTATACGTGTTCTGTGTGGGTGGAGAAAGACGGAGTAAATAGCTCTGTGGAGATTGTCACTAGTGCCAC AGCTCCCAACCCAGTGAGAAACCTGACAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAG GTCCCCGATGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAG GGACTCGAAGCACAGCACACACCAACATCACCGTGGATAGACTTGAACCCGGGTGTTTGTATGTGTTTTC CGTGTGGGTGGGGAAGAATGGAATCAACAGCTCCCGGGAGACTCGAAATGCCACCACAGCCCCCAACCCA GTGAGAAACCTCCATATGGAGACTCAGACCAACAGCTCCATCGCCCTATGCTGGGAAGTCCCCGATGGCC CATACCCTCAGGACTACACCTACTGGGTAGAGTACACTGGAGACGGTGGTGGCACAGAGACCCGAAACAC AACAAATACCAGTGTGACAGCTGAGAGACTTGAGCCCGGAACCTTGTACACATTCTCTGTATGGGCAGAA AAAAATGGAGCACGTGGCTCCAGGCAGAATGTCAGCATCTCCACAGTCCCCAACGCAGTGACAAGCCTCA GCAAGCAGGACTGGACCAACAGCACCATTGCTTTGCGCTGGACAGCTCCCCAGGGCCCAGGCCAGTCTTC CTACAGCTACTGGGTCTCATGGGTCAGGGAAGGCATGACTGACCCCAGGACCCAAAGCACCTCAGGTACT GACATCACCCTAAAGGAACTGGAAGCTGGCAGCCTGTACCACCTCACCGTCTGGGCCGAGAGGAATGAGG TCAGAGGCTATAACAGCACCCTCACTGCAGCCACTGCTCCCAATGAGGTCACAGATCTCCAGAATGAAAC TCAGACTAAGAACTCAGTCATGCTGTGGTGGAAGGCCCCTGGAGACCCCCACTCTCAGTTGTACGTATAC TGGGTCCAGTGGGCCAGCAAGGGACATCCCCGGAGGGGGCAAGATCCCCAAGCGAATTGGGTCAACCAGA CCAGCAGGACCAATGAGACGTGGTACAAAGTGGAGGCCCTGGAACCCGGGACGCTGTACAATTTCACCGT GTGGGCAGAGAGGAATGACGTAGCCAGTTCCACGCAGAGCCTCTGTGCGTCCACATACCCAGACACAGTC ACCATCACTTCCTGTGTCAGCACCTCAGCGGGCTATGGAGTCAACTTGATCTGGTCCTGCCCCCAGGGAG GCTACGAGGCCTTTGAGTTGGAGGTGGGAGGACAGCGGGGCTCCCAGGACAGATCTTCATGTGGGGAGGC TGTGTCTGTGTTGGGTCTCGGGCCGGCTCGGTCCTACCCAGCCACCATCACGACCATCTGGGACGGAATG AAGGTCGTGTCTCACTCTGTGGTCTGCCACACCGAGAGTGCAGGGGTCATTGCCGGAGCCTTTGTGGGCA TCCTCCTGTTTCTCATCCTCGTGGGCCTGCTGATTTTCTTCCTGAAGAGGAGGAATAAGAAGAAGCAGCA GAAACCAGAACTCAGGGATCTGGTCTTTAGCTCCCCAGGGGACATCCCAGCTGAAGACTTCGCTGACCAC GTCAGGAAGAATGAGAGGGACAGCAACTGTGGTTTTGCAGACGAGTACCAGCAACTCTCCCTGGTGGGCC ACAGCCAGTCTCAGATGGTGGCTTCGGCTTCAGAGAACAACGCCAAGAACCGCTACAGAAATGTGCTGCC CTATGACTGGTCCCGGGTGCCTCTGAAGCCCATCCATGAGGAGCCAGGCTCTGACTACATCAATGCCAGC TTCATGCCCGGTCTCTGGAGCCCCCAGGAGTTCATTGCAACCCAGGGTCCCCTGCCACAGACAGTGGGTG ACTTCTGGCGCCTGGTGTGGGAACAGCAGAGCCACACCCTGGTCATGCTGACCAACTGCATGGAGGCCGG CCGGGTGAAGTGTGAGCATTACTGGCCTCTGGACTCGCAGCCCTGCACCCATGGGCACCTGCGGGTAACC CTGGTAGATGAGGAAGTGATGGAGAACTGGACGGTGCGGGAACTGCTGCTCCTCCAGGTGGAGGAGCAGA AGACACTGTCTGTGCGCCAATTCCACTACCAGGCCTGGCCGGATCACGGCGTTCCCTCCTCCCCAGACAC CTTGCTGGCTTTCTGGAGGATGCTTCGGCAGTGGCTGGATCAGACCATGGAGGGAGGCCCACCCATTGTG CACTGCAGTGCTGGCGTGGGTCGCACAGGAACCCTCATTGCCCTGGACGTCCTGCTCCGGCAGCTGCAGT CCGAGGGTCTCCTTGGGCCCTTCAGCTTTGTAAGGAAGATGAGAGAGAGTCGGCCGTTGATGGTGCAGAC TGAGGCTCAGTACGTATTCCTGCATCAGTGCATCCTGCGGTTCCTCCAACAGTCAGCCCAGGCCCCAGCC GAGAAGGAAGTCCCGTATGAGGATGTCGAAAACCTCATCTACGAGAACGTGGCCGCCATCAAGGCCCACA AGTTGGAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229043 representing NM_001161440
Red=Cloning site Green=Tags(s) MAGAGGGLGVWGNLVLLGLCSWTGARAPAPNPGRNLTVETQTTSSISLSWEVPDGLDSQNSNYWVQCTGD GGTTETRNTTATNVTVDGLGPGSLYTCSVWVEKDGVNSSVEIVTSATAPNPVRNLTVEAQTNSSIALTWE VPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTNITVDRLEPGCLYVFSVWVGKNGINSSRETRNATTAPNP VRNLHMETQTNSSIALCWEVPDGPYPQDYTYWVEYTGDGGGTETRNTTNTSVTAERLEPGTLYTFSVWAE KNGARGSRQNVSISTVPNAVTSLSKQDWTNSTIALRWTAPQGPGQSSYSYWVSWVREGMTDPRTQSTSGT DITLKELEAGSLYHLTVWAERNEVRGYNSTLTAATAPNEVTDLQNETQTKNSVMLWWKAPGDPHSQLYVY WVQWASKGHPRRGQDPQANWVNQTSRTNETWYKVEALEPGTLYNFTVWAERNDVASSTQSLCASTYPDTV TITSCVSTSAGYGVNLIWSCPQGGYEAFELEVGGQRGSQDRSSCGEAVSVLGLGPARSYPATITTIWDGM KVVSHSVVCHTESAGVIAGAFVGILLFLILVGLLIFFLKRRNKKKQQKPELRDLVFSSPGDIPAEDFADH VRKNERDSNCGFADEYQQLSLVGHSQSQMVASASENNAKNRYRNVLPYDWSRVPLKPIHEEPGSDYINAS FMPGLWSPQEFIATQGPLPQTVGDFWRLVWEQQSHTLVMLTNCMEAGRVKCEHYWPLDSQPCTHGHLRVT LVDEEVMENWTVRELLLLQVEEQKTLSVRQFHYQAWPDHGVPSSPDTLLAFWRMLRQWLDQTMEGGPPIV HCSAGVGRTGTLIALDVLLRQLQSEGLLGPFSFVRKMRESRPLMVQTEAQYVFLHQCILRFLQQSAQAPA EKEVPYEDVENLIYENVAAIKAHKLEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001161440 |
ORF Size | 2811 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001161440.1, NM_001161440.2, NP_001154912.1 |
RefSeq ORF | 2814 bp |
Locus ID | 5794 |
Cytogenetics | 19q13.42 |
Protein Families | Druggable Genome, Transmembrane |
MW | 103.2 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]' |
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