SAP1 (PTPRH) (NM_001161440) Human Untagged Clone

CAT#: SC327678

PTPRH (untagged)-Human protein tyrosine phosphatase receptor type H (PTPRH) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRH
• Synonyms: R-PTP-H; SAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001161440, the custom clone sequence may differ by one or more nucleotides
  ATGGCTGGGGCTGGCGGGGGCCTCGGGGTCTGGGGGAACCTGGTGCTGCTGGGCCTGTGC
  AGCTGGACAGGGGCCAGGGCGCCTGCCCCCAACCCAGGGAGGAACCTGACAGTGGAGACT
  CAGACCACCAGCTCCATCTCCCTGAGCTGGGAGGTCCCCGATGGCCTAGACTCACAGAAC
  TCCAACTACTGGGTTCAGTGTACTGGAGACGGCGGCACAACAGAGACTCGAAACACAACA
  GCCACCAACGTCACCGTGGATGGCCTTGGACCCGGGTCATTGTATACGTGTTCTGTGTGG
  GTGGAGAAAGACGGAGTAAATAGCTCTGTGGGGACTGTCACTACTGCCACAGCTCCCAAC
  CCAGTGAGAAACCTGACAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAG
  GTCCCCGATGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGT
  GGCAGAGCAGGGACTCGAAGCACAGCACACACCAACATCACCGTGGATAGACTTGAACCC
  GGGTGTTTGTATGTGTTTTCCGTGTGGGTGGGGAAGAATGGAATCAACAGCTCCCGGGAG
  ACTCGAAATGCCACCACAGCCCCCAACCCAGTGAGAAACCTCCATATGGAGACTCAGACC
  AACAGCTCCATCGCCCTATGCTGGGAAGTCCCCGATGGCCCATACCCTCAGGACTACACC
  TACTGGGTAGAGTACACTGGAGACGGTGGTGGCACAGAGACCCGAAACACAACAAATACC
  AGTGTGACAGCTGAGAGACTTGAGCCCGGAACCTTGTACACATTCTCTGTATGGGCAGAA
  AAAAATGGAGCACGTGGCTCCAGGCAGAATGTCAGCATCTCCACAGTCCCCAACGCAGTG
  ACAAGCCTCAGCAAGCAGGACTGGACCAACAGCACCATTGCTTTGCGCTGGACAGCTCCC
  CAGGGCCCAGGCCAGTCTTCCTACAGCTACTGGGTCTCATGGGTCAGGGAAGGCATGACT
  GACCCCAGGACCCAAAGCACCTCAGGTACTGACATCACCCTAAAGGAACTGGAAGCTGGC
  AGCCTGTACCACCTCACCGTCTGGGCCGAGAGGAATGAGGTCAGAGGCTATAACAGCACC
  CTCACTGCAGCCACTGCTCCCAATGAGGTCACAGATCTCCAGAATGAAACTCAGACTAAG
  AACTCAGTCATGCTGTGGTGGAAGGCCCCTGGAGACCCCCACTCTCAGTTGTACGTATAC
  TGGGTCCAGTGGGCCAGCAAGGGACATCCCCGGAGGGGGCAAGATCCCCAAGCGAATTGG
  GTCAACCAGACCAGCAGGACCAATGAGACGTGGTACAAAGTGGAGGCCCTGGAACCCGGG
  ACGTTGTACAATTTCACCGTGTGGGCAGAGAGGAATGACGTAGCCAGTTCCACGCAGAGC
  CTCTGTGCGTCCACATACCCAGACACAGTCACCATCACTTCCTGTGTCAGCACCTCAGCG
  GGCTATGGAGTCAACTTGATCTGGTCCTGCCCCCAGGGAGGCTACGAGGCCTTTGAGTTG
  GAGGTGGGAGGACAGCGGGGCTCCCAGGACAGATCTTCATGTGGGGAGGCTGTGTCTGTG
  TTGGGTCTCGGGCCGGCTCGGTCCTACCCAGCCACCATCACGACCATCTGGGACGGAATG
  AAGGTCGTGTCTCACTCTGTGGTCTGCCACACCGAGAGTGCAGGGGTCATTGCCGGAGCC
  TTTGTGGGCATCCTCCTGTTTCTCATCCTCGTGGGCCTGCTGATTTTCTTCCTGAAGAGG
  AGGAATAAGAAGAAGCAGCAGAAACCAGAACTCAGGGATCTGGTCTTTAGCTCCCCAGGG
  GACATCCCAGCTGAAGACTTCGCTGACCACGTCAGGAAGAATGAGAGGGACAGCAACTGT
  GGTTTTGCAGACAAGTACCAGCAACTCTCCCTGGTGGGCCACAGCCAGTCTCAGATGGTG
  GCTTCGGCTTCAGAGAACAACGCCAAGAACCGCTACAGAAATGTGCTGCCCTATGACTGG
  TCCCGGGTGCCCCTGAAGCCCATCCATGAGGAGCCAGGCTCTGACTACATCAATGCCAGC
  TTCATGCCCGGTCTCTGGAGCCCCCAGGAGTTCATTGCAACCCAGGGTCCCCTGCCACAG
  ACAGTGGGTGACTTCTGGCGCCTGGTGTGGGAACAGCAGAGCCACACCCTGGTCATGCTG
  ACCAACTGCATGGAGGCCGGCCGGGTGAAGTGTGAGCATTACTGGCCTCTGGACTCGCAG
  CCCTGCACCCATGGGCACCTGCGGGTAACCCTGGTAGGTGAGGAAGTGATGGAGAACTGG
  ACGGTGCGGGAACTGCTGCTCCTCCAGGTGGAGGAGCAGAAGACACTGTCTGTGCGCCAA
  TTCCACTACCAGGCCTGGCCGGATCACGGCGTTCCCTCCTCCCCAGACACCTTGCTGGCT
  TTCTGGAGGATGCTTCGGCAGTGGCTGGATCAGACCATGGAGGGAGGCCCACCCATTGTG
  CACTGCAGTGCTGGCGTGGGTCGCACAGGAACCCTCATTGCCCTGGACGTCCTGCTCCGG
  CAGCTGCAGTCCGAGGGTCTCCTTGGGCCCTTCAGCTTTGTAAGGAAGATGAGAGAGAGT
  CGGCCGTTGATGGTGCAGACTGAGGCTCAGTACGTATTCCTGCATCAGTGCATCCTGCGG
  TTCCTCCAACAGTCAGCCCAGGCCCCAGCCGAGAAGGAAGTCCCGTATGAGGATGTCGAA
  AACCTCATCTACGAGAACGTGGCCGCCATCCAGGCCCACAAGTTGGAGGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001161440
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161440.1, NP_001154912.1
• RefSeq Size: 3401 bp
• RefSeq ORF: 2814 bp
• Locus ID: 5794
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]'
  Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.