SAP1 (PTPRH) (NM_001161440) Human Tagged ORF Clone

CAT#: RG229043

PTPRH (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, H (PTPRH), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRH
• Synonyms: R-PTP-H; SAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229043 representing NM_001161440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGCTGGCGGGGGCCTCGGGGTCTGGGGGAACCTGGTGCTGCTGGGCCTGTGCAGCTGGACAG
  GGGCCAGGGCGCCTGCCCCCAACCCAGGGAGGAACCTGACAGTGGAGACTCAGACCACCAGCTCCATCTC
  CCTGAGCTGGGAGGTCCCCGATGGCCTAGACTCACAGAACTCCAACTACTGGGTTCAGTGTACTGGAGAC
  GGCGGCACAACAGAGACTCGAAACACAACAGCCACCAACGTCACCGTGGATGGCCTTGGACCCGGGTCAT
  TGTATACGTGTTCTGTGTGGGTGGAGAAAGACGGAGTAAATAGCTCTGTGGAGATTGTCACTAGTGCCAC
  AGCTCCCAACCCAGTGAGAAACCTGACAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAG
  GTCCCCGATGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAG
  GGACTCGAAGCACAGCACACACCAACATCACCGTGGATAGACTTGAACCCGGGTGTTTGTATGTGTTTTC
  CGTGTGGGTGGGGAAGAATGGAATCAACAGCTCCCGGGAGACTCGAAATGCCACCACAGCCCCCAACCCA
  GTGAGAAACCTCCATATGGAGACTCAGACCAACAGCTCCATCGCCCTATGCTGGGAAGTCCCCGATGGCC
  CATACCCTCAGGACTACACCTACTGGGTAGAGTACACTGGAGACGGTGGTGGCACAGAGACCCGAAACAC
  AACAAATACCAGTGTGACAGCTGAGAGACTTGAGCCCGGAACCTTGTACACATTCTCTGTATGGGCAGAA
  AAAAATGGAGCACGTGGCTCCAGGCAGAATGTCAGCATCTCCACAGTCCCCAACGCAGTGACAAGCCTCA
  GCAAGCAGGACTGGACCAACAGCACCATTGCTTTGCGCTGGACAGCTCCCCAGGGCCCAGGCCAGTCTTC
  CTACAGCTACTGGGTCTCATGGGTCAGGGAAGGCATGACTGACCCCAGGACCCAAAGCACCTCAGGTACT
  GACATCACCCTAAAGGAACTGGAAGCTGGCAGCCTGTACCACCTCACCGTCTGGGCCGAGAGGAATGAGG
  TCAGAGGCTATAACAGCACCCTCACTGCAGCCACTGCTCCCAATGAGGTCACAGATCTCCAGAATGAAAC
  TCAGACTAAGAACTCAGTCATGCTGTGGTGGAAGGCCCCTGGAGACCCCCACTCTCAGTTGTACGTATAC
  TGGGTCCAGTGGGCCAGCAAGGGACATCCCCGGAGGGGGCAAGATCCCCAAGCGAATTGGGTCAACCAGA
  CCAGCAGGACCAATGAGACGTGGTACAAAGTGGAGGCCCTGGAACCCGGGACGCTGTACAATTTCACCGT
  GTGGGCAGAGAGGAATGACGTAGCCAGTTCCACGCAGAGCCTCTGTGCGTCCACATACCCAGACACAGTC
  ACCATCACTTCCTGTGTCAGCACCTCAGCGGGCTATGGAGTCAACTTGATCTGGTCCTGCCCCCAGGGAG
  GCTACGAGGCCTTTGAGTTGGAGGTGGGAGGACAGCGGGGCTCCCAGGACAGATCTTCATGTGGGGAGGC
  TGTGTCTGTGTTGGGTCTCGGGCCGGCTCGGTCCTACCCAGCCACCATCACGACCATCTGGGACGGAATG
  AAGGTCGTGTCTCACTCTGTGGTCTGCCACACCGAGAGTGCAGGGGTCATTGCCGGAGCCTTTGTGGGCA
  TCCTCCTGTTTCTCATCCTCGTGGGCCTGCTGATTTTCTTCCTGAAGAGGAGGAATAAGAAGAAGCAGCA
  GAAACCAGAACTCAGGGATCTGGTCTTTAGCTCCCCAGGGGACATCCCAGCTGAAGACTTCGCTGACCAC
  GTCAGGAAGAATGAGAGGGACAGCAACTGTGGTTTTGCAGACGAGTACCAGCAACTCTCCCTGGTGGGCC
  ACAGCCAGTCTCAGATGGTGGCTTCGGCTTCAGAGAACAACGCCAAGAACCGCTACAGAAATGTGCTGCC
  CTATGACTGGTCCCGGGTGCCTCTGAAGCCCATCCATGAGGAGCCAGGCTCTGACTACATCAATGCCAGC
  TTCATGCCCGGTCTCTGGAGCCCCCAGGAGTTCATTGCAACCCAGGGTCCCCTGCCACAGACAGTGGGTG
  ACTTCTGGCGCCTGGTGTGGGAACAGCAGAGCCACACCCTGGTCATGCTGACCAACTGCATGGAGGCCGG
  CCGGGTGAAGTGTGAGCATTACTGGCCTCTGGACTCGCAGCCCTGCACCCATGGGCACCTGCGGGTAACC
  CTGGTAGATGAGGAAGTGATGGAGAACTGGACGGTGCGGGAACTGCTGCTCCTCCAGGTGGAGGAGCAGA
  AGACACTGTCTGTGCGCCAATTCCACTACCAGGCCTGGCCGGATCACGGCGTTCCCTCCTCCCCAGACAC
  CTTGCTGGCTTTCTGGAGGATGCTTCGGCAGTGGCTGGATCAGACCATGGAGGGAGGCCCACCCATTGTG
  CACTGCAGTGCTGGCGTGGGTCGCACAGGAACCCTCATTGCCCTGGACGTCCTGCTCCGGCAGCTGCAGT
  CCGAGGGTCTCCTTGGGCCCTTCAGCTTTGTAAGGAAGATGAGAGAGAGTCGGCCGTTGATGGTGCAGAC
  TGAGGCTCAGTACGTATTCCTGCATCAGTGCATCCTGCGGTTCCTCCAACAGTCAGCCCAGGCCCCAGCC
  GAGAAGGAAGTCCCGTATGAGGATGTCGAAAACCTCATCTACGAGAACGTGGCCGCCATCAAGGCCCACA
  AGTTGGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229043 representing NM_001161440
  Red=Cloning site Green=Tags(s)
  
  MAGAGGGLGVWGNLVLLGLCSWTGARAPAPNPGRNLTVETQTTSSISLSWEVPDGLDSQNSNYWVQCTGD
  GGTTETRNTTATNVTVDGLGPGSLYTCSVWVEKDGVNSSVEIVTSATAPNPVRNLTVEAQTNSSIALTWE
  VPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTNITVDRLEPGCLYVFSVWVGKNGINSSRETRNATTAPNP
  VRNLHMETQTNSSIALCWEVPDGPYPQDYTYWVEYTGDGGGTETRNTTNTSVTAERLEPGTLYTFSVWAE
  KNGARGSRQNVSISTVPNAVTSLSKQDWTNSTIALRWTAPQGPGQSSYSYWVSWVREGMTDPRTQSTSGT
  DITLKELEAGSLYHLTVWAERNEVRGYNSTLTAATAPNEVTDLQNETQTKNSVMLWWKAPGDPHSQLYVY
  WVQWASKGHPRRGQDPQANWVNQTSRTNETWYKVEALEPGTLYNFTVWAERNDVASSTQSLCASTYPDTV
  TITSCVSTSAGYGVNLIWSCPQGGYEAFELEVGGQRGSQDRSSCGEAVSVLGLGPARSYPATITTIWDGM
  KVVSHSVVCHTESAGVIAGAFVGILLFLILVGLLIFFLKRRNKKKQQKPELRDLVFSSPGDIPAEDFADH
  VRKNERDSNCGFADEYQQLSLVGHSQSQMVASASENNAKNRYRNVLPYDWSRVPLKPIHEEPGSDYINAS
  FMPGLWSPQEFIATQGPLPQTVGDFWRLVWEQQSHTLVMLTNCMEAGRVKCEHYWPLDSQPCTHGHLRVT
  LVDEEVMENWTVRELLLLQVEEQKTLSVRQFHYQAWPDHGVPSSPDTLLAFWRMLRQWLDQTMEGGPPIV
  HCSAGVGRTGTLIALDVLLRQLQSEGLLGPFSFVRKMRESRPLMVQTEAQYVFLHQCILRFLQQSAQAPA
  EKEVPYEDVENLIYENVAAIKAHKLEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161440
• ORF Size: 2811 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161440.1, NP_001154912.1
• RefSeq Size: 3401 bp
• RefSeq ORF: 2814 bp
• Locus ID: 5794
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]'