C6orf134 (ATAT1) (NM_024909) Human Tagged ORF Clone

CAT#: RC229162

ATAT1 (Myc-DDK-tagged)-Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY432186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229162 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATAT1
• Synonyms: alpha-TAT; alpha-TAT1; C6orf134; MEC17; Nbla00487; TAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229162 representing NM_024909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCCCGTTCGATGTGGACGCGCTGTTCCCGGAGCGGATCACGGTGCTGGACCAGCACCTGAGGC
  CCCCAGCCCGCCGACCCGGAACCACAACGCCGGCCCGTGTTGATCTACAGCAGCAAATTATGACCATTAT
  AGATGAACTGGGCAAGGCTTCTGCCAAGGCCCAGAATCTTTCCGCTCCTATCACTAGTGCATCAAGGATG
  CAGAGTAACCGCCATGTTGTTTATATTCTCAAAGACAGTTCAGCCCGACCGGCTGGAAAAGGAGCCATTA
  TTGGTTTCATCAAAGTTGGATACAAGAAGCTCTTTGTACTGGATGATCGTGAGGCTCATAATGAGGTAGA
  ACCACTTTGCATCCTGGACTTTTACATCCATGAGTCTGTGCAACGCCATGGCCATGGGCGAGAACTCTTC
  CAGTATATGTTGCAGAAGGAGCGAGTGGAACCGCACCAACTGGCAATTGACCGACCCTCACAGAAGCTGC
  TGAAATTCCTGAATAAGCACTACAATCTGGAGACCACAGTCCCACAGGTGAACAACTTTGTGATCTTTGA
  AGGCTTCTTTGCCCATCAACATCGGCCCCCTGCTCCCTCTCTGAGGGCAACTCGACACTCTCGTGCTGCT
  GCAGTCGATCCCACGCCCGCTGCTCCAGCAAGGAAGCTGCCACCCAAGAGAGCAGAGGGAGACATCAAGC
  CATACTCCTCTAGTGACCGAGAATTTCTGAAGGTAGCTGTGGAGCCTCCTTGGCCCCTAAACAGGGCCCC
  TCGCCGCGCCACACCTCCAGCCCACCCACCCCCCCGCTCCAGCAGCCTGGGAAACTCACCAGAACGAGGT
  CCCCTCCGCCCCTTTGTGCCAGAGCAGGAGCTGCTGCGTTCCTTGCGCCTCTGCCCCCCACACCCTACCG
  CCCGCCTTCTGTTGGCTGCTGACCCTGGGGGCAGCCCAGCTCAACGTCGTCGCACCAGCTCCCTTCCCCG
  CTCTGAGGAGAGTCGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229162 representing NM_024909
  Red=Cloning site Green=Tags(s)
  
  MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRM
  QSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELF
  QYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAA
  AVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERG
  PLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTSSLPRSEESRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024909
• ORF Size: 999 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024909.2, NM_024909.3, NP_079185.2
• RefSeq ORF: 1002
• Locus ID: 79969
• MW: 37.9 kDa
• Gene Summary: This gene encodes a protein that localizes to clathrin-coated pits, where it acetylates alpha tubulin on lysine 40. This process may be important in microtubule growth, for instance during chemotaxis and the formation of cilium. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]