Eph receptor B6 (EPHB6) (NM_004445) Human Tagged ORF Clone

CAT#: RC229404

EPHB6 (Myc-DDK-tagged)-Human EPH receptor B6 (EPHB6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPHB6
• Synonyms: HEP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229404 representing NM_004445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGGCATGGTGTGTAGCCTATGGGTGCTGC
  TCCTGGTGTCTTCAGTTCTGGCTCTGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGG
  CTGGCTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCAGCGACGCCTGACTCGG
  ACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTCCAGGCACCGGGCAGGACAATTGGTTGCAGACACACT
  TTGTGGAGCGGCGCGGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTCCAGCCT
  GGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACTACCGTCAGGCTGAGGAGCCCGACAGC
  CCTGACAGCGTTTCCTCCTGGCACCTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCT
  TTCCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGGGACCCCACGGGGC
  TGGGCAGCGGGCTGGACTGCAACTGAACGTCAAAGAGCGGAGCTTTGGGCCTCTCACCCAACGCGGCTTC
  TACGTGGCCTTCCAGGACACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCTGCC
  CTGCCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGTGGGGCTGGGGGGGCCTCCCTGGT
  GGCAGCTGTGGGCACCTGTGTGGCTCATGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGC
  AGCCCCCCCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGCTGCCGCTGCCAGC
  CTGGATACCAACCAGCACGAGGAGACAAGGCCTGCCAAGCCTGCCCACGGGGGCTCTATAAGGCTTCTGC
  TGGGAATGCTCCCTGCTCACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGCCCC
  TGCCTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCCCTGCACTGGTCCTCCATCGGCTC
  CCCAGGAGCTTTGGTTTGAGGTGCAAGGCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGG
  GGGTCGAGGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGAACCTGCCAGCGGT
  GGTGGGGGCACTTGTCACCGCTGCAGGGATGAGGTCCACTTCGACCCTCGCCAGAGAGGCCTGACTGAGA
  GCCGAGTGTTAGTGGGGGGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGGGGT
  GTCTGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCAGCACCAGCCATGAAGTGCCCTCT
  GCTGTCCCTGTGGTGCACCAGGTGAGCCGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACC
  AGACCAATGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACGAATCCCACTCCTT
  CACCCTGACCAGCGAGACCAACACTGCCACCGTGACACAGCTGAGCCCTGGCCACATCTATGGTTTCCAG
  GTGCGGGCCCGGACTGCTGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTCAAG
  GGGAGCTGTCTTCCCAGCTTCCAGAAAGACTCTCCTTGGTGATCGGCTCCATCCTGGGGGCTTTGGCCTT
  CCTCCTGCTGGCAGCCATCACCGTGCTGGCGGTCGTCTTCCAGCGGAAGCGGCGTGGGACTGGCTACACA
  GAGCAGCTGCAGCAATACAGCAGCCCAGGACTCGGGGTGAAGTATTACATCGACCCCTCCACCTACGAGG
  ACCCCTGTCAGGCCATCCGAGAACTTGCCCGGGAAGTCGATCCTGCTTATATCAAGATTGAGGAGGTCAT
  TGGGACAGGCTCTTTTGGAGAAGTGCGCCAGGGCCGCCTGCAGCCACGGGGACGGAGGGAGCAGACTGTG
  GCCATCCAGGCCCTGTGGGCCGGGGGCGCCGAAAGCCTGCAGATGACCTTCCTGGGCCGGGCCGCAGTGC
  TGGGTCAGTTCCAGCACCCCAACATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCATGGT
  GCTGACGGAGTTCATGGAGCTTGGCCCCCTGGACAGCTTCCTCAGGCAGCGGGAGGGCCAGTTCAGCAGC
  CTGCAGCTGGTGGCCATGCAGCGGGGAGTGGCTGCTGCCATGCAGTACCTGTCCAGCTTTGCCTTCGTCC
  ATCGCTCGCTGTCTGCCCACAGCGTGCTGGTGAATAGCCACTTGGTGTGCAAGGTGGCCCGTCTTGGCCA
  CAGTCCTCAGGGCCCAAGTTGTTTGCTTCGCTGGGCAGCCCCAGAGGTCATTGCACATGGAAAGCATACA
  ACATCCAGTGATGTCTGGAGCTTTGGGATACTCATGTGGGAAGTGATGAGTTATGGAGAACGGCCTTACT
  GGGACATGAGTGAGCAGGAGGTACTAAATGCAATAGAGCAGGAGTTCCGGCTGCCCCCGCCTCCAGGCTG
  TCCTCCTGGATTACATCTACTTATGTTGGACACTTGGCAGAAGGACCGTGCCCGGCGGCCTCATTTTGAC
  CAGCTGGTGGCTGCATTTGACAAGATGATCCGCAAGCCAGATACCCTGCAGGCTGGCGGGGACCCAGGGG
  AAAGGCCTTCCCAGGCCCTTCTGACCCCTGTGGCCCTGGACTTTCCTTGTCTGGACTCACCCCAGGCCTG
  GCTTTCAGCCATTGGACTGGAGTGCTACCAGGACAACTTCTCCAAGTTTGGCCTCTGTACCTTCAGTGAT
  GTGGCTCAGCTCAGCCTAGAAGACCTGCCTGCCCTGGGCATCACCCTGGCTGGCCACCAGAAGAAGCTGC
  TGCACCACATCCAGCTCCTTCAGCAACACCTGAGGCAGCAGGGCTCAGTGGAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229404 representing NM_004445
  Red=Cloning site Green=Tags(s)
  
  MATEGAAQLGNRVAGMVCSLWVLLLVSSVLALEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTR
  TFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDS
  PDSVSSWHLKRWTKVDTIAADESFPSSSSSSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGF
  YVAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGG
  SPPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPRGLYKASAGNAPCSPCPARSHAPNPAAPVCP
  CLEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASG
  GGGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPS
  AVPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQ
  VRARTAAGHGPYGGKVYFQTLPQGELSSQLPERLSLVIGSILGALAFLLLAAITVLAVVFQRKRRGTGYT
  EQLQQYSSPGLGVKYYIDPSTYEDPCQAIRELAREVDPAYIKIEEVIGTGSFGEVRQGRLQPRGRREQTV
  AIQALWAGGAESLQMTFLGRAAVLGQFQHPNILRLEGVVTKSRPLMVLTEFMELGPLDSFLRQREGQFSS
  LQLVAMQRGVAAAMQYLSSFAFVHRSLSAHSVLVNSHLVCKVARLGHSPQGPSCLLRWAAPEVIAHGKHT
  TSSDVWSFGILMWEVMSYGERPYWDMSEQEVLNAIEQEFRLPPPPGCPPGLHLLMLDTWQKDRARRPHFD
  QLVAAFDKMIRKPDTLQAGGDPGERPSQALLTPVALDFPCLDSPQAWLSAIGLECYQDNFSKFGLCTFSD
  VAQLSLEDLPALGITLAGHQKKLLHHIQLLQQHLRQQGSVEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004445
• ORF Size: 3066 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004445.3, NM_004445.4, NM_004445.5, NP_004436.2, NP_004436.4
• RefSeq Size: 3449 bp
• RefSeq ORF: 3069 bp
• Locus ID: 2051
• Cytogenetics: 7q34
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 110.8 kDa
• Gene Summary: 'This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'