Eph receptor B6 (EPHB6) (NM_004445) Human Tagged ORF Clone

CAT#: RC600052

EPHB6 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor B6, Signal peptide (1-31) plus EC domain (32-594)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: EPHB6
• Synonyms: HEP
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600052 representing leader sequence plus the extracellular domain region of NM_004445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGGCATGGTGTGTAGCCTATGGGTGCTGC
  TCCTGGTGTCTTCAGTTCTGGCTCTGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGG
  CTGGCTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCAGCGACGCCTGACTCGG
  ACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTCCAGGCACCGGGCAGGACAATTGGTTGCAGACACACT
  TTGTGGAGCGGCGCGGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTCCAGCCT
  GGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACTACCGTCAGGCTGAGGAGCCCGACAGC
  CCTGACAGCGTTTCCTCCTGGCACCTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCT
  TTCCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGGGACCCCACGGGGCTGG
  GCAGCGGGCTGGACTGCAACTGAACGTCAAAGAGCGGAGCTTTGGGCCTCTCACCCAACGCGGCTTCTAC
  GTGGCCTTCCAGGACACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCTGCCCTG
  CCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGTGGGGCTGGGGGGGCCTCCCTGGTGGC
  AGCTGTGGGCACCTGTGTGGCTCATGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGCAGC
  CCCCCCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGCTGCCGCTGCCAGCCTG
  GATACCAACCAGCACGAGGAGACAAGGCCTGCCAAGCCTGCCCACGGGGGCTCTATAAGTCTTCTGCTGG
  GAATGCTCCCTGCTCACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGCCCCTGC
  CTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCCCTGCACTGGTCCTCCATCGGCTCCCC
  AGGAGCTTTGGTTTGAGGTGCAAGGCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGGGGG
  TCGAGGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGAACCTGCCAGCGGTGGT
  GGGGGCACTTGTCACCGCTGCAGGGATGAGGTCCACTTCGACCCTCGCCAGAGAGGCCTGACTGAGAGCC
  GAGTGTTAGTGGGGGGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGGGGTGTC
  TGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCAGCACCAGCCATGAAGTGCCCTCTGCT
  GTCCCTGTGGTGCACCAGGTGAGCCGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACCAGA
  CCAATGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACGAATCCCACTCCTTCAC
  CCTGACCAGCGAGACCAACACTGCCACCGTGACACAGCTGAGCCCTGGCCACATCTATGGTTTCCAGGTG
  CGGGCCCGGACTGCTGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTCAAGGGG
  AGCTGTCTTCCCAGCTTCCGGAAAGACTCTCC
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600052 representing signal peptide plus the extracellular domain region of NM_004445
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MATEGAAQLGNRVAGMVCSLWVLLLVSSVLALEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTR
  TFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDS
  PDSVSSWHLKRWTKVDTIAADESFPSSSSSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFY
  VAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGS
  PPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPRGLYKSSAGNAPCSPCPARSHAPNPAAPVCPC
  LEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASGG
  GGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSA
  VPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQV
  RARTAAGHGPYGGKVYFQTLPQGELSSQLPERLS
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_004445
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004445.5, NP_004436.4
• RefSeq Size: 3449 bp
• RefSeq ORF: 3069 bp
• Locus ID: 2051
• Cytogenetics: 7q34
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 63.3 kDa
• Gene Summary: 'This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'