Eph receptor B6 (EPHB6) (NM_004445) Human Tagged ORF Clone

CAT#: RG229404

EPHB6 (GFP-tagged) - Human EPH receptor B6 (EPHB6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPHB6
• Synonyms: HEP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229404 representing NM_004445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGGCATGGTGTGTAGCCTATGGGTGCTGC
  TCCTGGTGTCTTCAGTTCTGGCTCTGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGG
  CTGGCTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCAGCGACGCCTGACTCGG
  ACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTCCAGGCACCGGGCAGGACAATTGGTTGCAGACACACT
  TTGTGGAGCGGCGCGGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTCCAGCCT
  GGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACTACCGTCAGGCTGAGGAGCCCGACAGC
  CCTGACAGCGTTTCCTCCTGGCACCTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCT
  TTCCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGGGACCCCACGGGGCTGG
  GCAGCGGGCTGGACTGCAACTGAACGTCAAAGAGCGGAGCTTTGGGCCTCTCACCCAACGCGGCTTCTAC
  GTGGCCTTCCAGGACACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCTGCCCTG
  CCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGTGGGGCTGGGGGGGCCTCCCTGGTGGC
  AGCTGTGGGCACCTGTGTGGCTCATGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGCAGC
  CCCCCCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGCTGCCGCTGCCAGCCTG
  GATACCAACCAGCACGAGGAGACAAGGCCTGCCAAGCCTGCCCACGGGGGCTCTATAAGTCTTCTGCTGG
  GAATGCTCCCTGCTCACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGCCCCTGC
  CTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCCCTGCACTGGTCCTCCATCGGCTCCCC
  AGGAGCTTTGGTTTGAGGTGCAAGGCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGGGGG
  TCGAGGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGAACCTGCCAGCGGTGGT
  GGGGGCACTTGTCACCGCTGCAGGGATGAGGTCCACTTCGACCCTCGCCAGAGAGGCCTGACTGAGAGCC
  GAGTGTTAGTGGGGGGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGGGGTGTC
  TGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCAGCACCAGCCATGAAGTGCCCTCTGCT
  GTCCCTGTGGTGCACCAGGTGAGCCGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACCAGA
  CCAATGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACGAATCCCACTCCTTCAC
  CCTGACCAGCGAGACCAACACTGCCACCGTGACACAGCTGAGCCCTGGCCACATCTATGGTTTCCAGGTG
  CGGGCCCGGACTGCTGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTCAAGGGG
  AGCTGTCTTCCCAGCTTCCGGAAAGACTCTCCTTGGTGATCGGCTCCATCCTGGGGGCTTTGGCCTTCCT
  CCTGCTGGCAGCCATCACCGTGCTGGCGGTCGTCTTCCAGCGGAAGCGGCGTGGGACTGGCTACACGGAG
  CAGCTGCAGCAATACAGCAGCCCAGGACTCGGGGTGAAGTATTACATCGACCCCTCCACCTACGAGGACC
  CCTGTCAGGCCATCCGAGAACTTGCCCGGGAAGTCGATCCTGCTTATATCAAGATTGAGGAGGTCATTGG
  GACAGGCTCTTTTGGAGAAGTGCGCCAGGGCCGCCTGCAGCCACGGGGACGGAGGGAGCAGACTGTGGCC
  ATCCAGGCCCTGTGGGCCGGGGGCGCCGAAAGCCTGCAGATGACCTTCCTGGGCCGGGCCGCAGTGCTGG
  GTCAGTTCCAGCACCCCAACATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCATGGTGCT
  GACGGAGTTCATGGAGCTTGGCCCCCTGGACAGCTTCCTCAGGCAGCGGGAGGGCCAGTTCAGCAGCCTG
  CAGCTGGTGGCCATGCAGCGGGGAGTGGCTGCTGCCATGCAGTACCTGTCCAGCTTTGCCTTCGTCCATC
  GCTCGCTGTCTGCCCACAGCGTGCTGGTGAATAGCCACTTGGTGTGCAAGGTGGCCCGTCTTGGCCACAG
  TCCTCAGGGCCCAAGTTGTTTGCTTCGCTGGGCAGCCCCAGAGGTCATTGCACATGGAAAGCATACAACA
  TCCAGTGATGTCTGGAGCTTTGGGATACTCATGTGGGAAGTGATGAGTTATGGAGAACGGCCTTACTGGG
  ACATGAGTGAGCAGGAGGTACTAAATGCAATAGAGCAGGAGTTCCGGCTGCCCCCGCCTCCAGGCTGTCC
  TCCTGGATTACATCTACTTATGTTGGACACTTGGCAGAAGGACCGTGCCCGGCGGCCTCATTTTGACCAG
  CTGGTGGCTGCATTTGACAAGATGATCCGCAAGCCAGATACCCTGCAGGCTGGCGGGGACCCAGGGGAAA
  GGCCTTCCCAGGCCCTTCTGACCCCTGTGGCCCTGGACTTTCCTTGTCTGGACTCACCCCAGGCCTGGCT
  TTCAGCCATTGGACTGGAGTGCTACCAGGACAACTTCTCCAAGTTTGGCCTCTGTACCTTCAGTGATGTG
  GCTCAGCTCAGCCTAGAAGACCTGCCTGCCCTGGGCATCACCCTGGCTGGCCACCAGAAGAAGCTGCTGC
  ACCACATCCAGCTCCTTCAGCAACACCTGAGGCAGCAGGGCTCAGTGGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229404 representing NM_004445
  Red=Cloning site Green=Tags(s)
  
  MATEGAAQLGNRVAGMVCSLWVLLLVSSVLALEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTR
  TFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDS
  PDSVSSWHLKRWTKVDTIAADESFPSSSSSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFY
  VAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGS
  PPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPRGLYKSSAGNAPCSPCPARSHAPNPAAPVCPC
  LEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASGG
  GGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSA
  VPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQV
  RARTAAGHGPYGGKVYFQTLPQGELSSQLPERLSLVIGSILGALAFLLLAAITVLAVVFQRKRRGTGYTE
  QLQQYSSPGLGVKYYIDPSTYEDPCQAIRELAREVDPAYIKIEEVIGTGSFGEVRQGRLQPRGRREQTVA
  IQALWAGGAESLQMTFLGRAAVLGQFQHPNILRLEGVVTKSRPLMVLTEFMELGPLDSFLRQREGQFSSL
  QLVAMQRGVAAAMQYLSSFAFVHRSLSAHSVLVNSHLVCKVARLGHSPQGPSCLLRWAAPEVIAHGKHTT
  SSDVWSFGILMWEVMSYGERPYWDMSEQEVLNAIEQEFRLPPPPGCPPGLHLLMLDTWQKDRARRPHFDQ
  LVAAFDKMIRKPDTLQAGGDPGERPSQALLTPVALDFPCLDSPQAWLSAIGLECYQDNFSKFGLCTFSDV
  AQLSLEDLPALGITLAGHQKKLLHHIQLLQQHLRQQGSVEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004445
• ORF Size: 3063 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004445.3, NP_004436.2
• RefSeq Size: 4059 bp
• RefSeq ORF: 3069 bp
• Locus ID: 2051
• Cytogenetics: 7q34
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'