CHST11 (NM_001173982) Human Tagged ORF Clone

CAT#: RC229922

CHST11 (Myc-DDK-tagged)-Human carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY432922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229922 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHST11
• Synonyms: C4ST; C4ST-1; C4ST1; HSA269537; OCBMD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229922 representing NM_001173982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGCGCTGCTGGAAGTGATGAGGATGAACAGAATCTGCCGGATGGTGCTGGCCACTTGCTTGG
  GATCCTTTATCCTGGTCATCTTCTATTTCCAAATCATGCGGAGGAATCCCTTTGGTGTGGACATCTGCTG
  CCGGAAGGGGTCCCGAAGCCCCCTGCAGGAACTCTACAACCCAATCCAGCTGGAGCTCTCAAACACTGCT
  GTCCTGCACCAGATGCGGCGGGACCAGGTGACAGACACGTGCCGAGCCAACAGCGCCACAAGCCGTAAGC
  GGAGGGTGCTGACCCCCAACGACCTGAAGCACTTGGTGGTGGATGAGGACCACGAGCTCATCTACTGCTA
  CGTGCCCAAGGTGGCCTGCACCAACTGGAAGCGGCTCATGATGGTCCTGACCGGGCGGGGGAAGTACAGC
  GACCCCATGGAGATCCCGGCCAACGAGGCACACGTCTCCGCCAACCTGAAGACCCTGAACCAGTACAGCA
  TCCCAGAAATCAACCACCGCTTGAAAAGCTACATGAAGTTCCTGTTTGTCCGGGAGCCCTTCGAGAGGCT
  AGTGTCCGCCTACCGCAACAAGTTCACCCAGAAGTACAACATCTCCTTCCACAAGCGGTACGGCACCAAG
  ATCATCAAACGCCAGCGGAAGAACGCCACCCAGGAGGCCCTGCGCAAAGGGGACGATGTCAAATTCGAGG
  AGTTTGTGGCCTATCTCATCGACCCACACACCCAGCGGGAGGAGCCTTTCAACGAACACTGGCAAACCGT
  CTACTCACTCTGCCATCCCTGCCACATCCACTATGACCTCGTGGGCAAGTACGAGACACTGGAAGAGGAT
  TCTAATTACGTCCTGCAGCTGGCAGGAGTGGGCAGCTACCTGAAGTTCCCCACCTATGCAAAGTCTACGA
  GAACTACTGATGAAATGACCACAGAATTCTTCCAGAACATCAGCTCAGAGCACCAAACGCAGCTGTACGA
  AGTCTACAAACTCGATTTTTTAATGTTCAATTACTCAGTGCCAAGCTACCTGAAATTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229922 representing NM_001173982
  Red=Cloning site Green=Tags(s)
  
  MKPALLEVMRMNRICRMVLATCLGSFILVIFYFQIMRRNPFGVDICCRKGSRSPLQELYNPIQLELSNTA
  VLHQMRRDQVTDTCRANSATSRKRRVLTPNDLKHLVVDEDHELIYCYVPKVACTNWKRLMMVLTGRGKYS
  DPMEIPANEAHVSANLKTLNQYSIPEINHRLKSYMKFLFVREPFERLVSAYRNKFTQKYNISFHKRYGTK
  IIKRQRKNATQEALRKGDDVKFEEFVAYLIDPHTQREEPFNEHWQTVYSLCHPCHIHYDLVGKYETLEED
  SNYVLQLAGVGSYLKFPTYAKSTRTTDEMTTEFFQNISSEHQTQLYEVYKLDFLMFNYSVPSYLKLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173982
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001173982.1, NP_001167453.1
• RefSeq ORF: 1044
• Locus ID: 50515
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Sulfur metabolism
• MW: 41.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving this gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]