GGA3 (NM_001172704) Human Tagged ORF Clone

CAT#: RC230320

GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY433320) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230320 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA3
• Synonyms: KIAA0154
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230320 representing NM_001172704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCCAGAAGAAGCAAAGATCAAAGACGCCTACCACATGCTGAAGAGACAGGGCATAGTGCAGT
  CTGACCCACCAATTCCTGTGGATAGGACGCTGATCCCCTCTCCACCACCTCGTCCCAAAAACCCTGTTTT
  TGATGATGAGGAGAAGTCCAAGCTTTTAGCCAAGCTGCTGAAAAGCAAAAACCCAGATGACCTGCAGGAG
  GCCAACAAGCTCATCAAGTCCATGGTGAAGGAAGACGAGGCACGGATCCAGAAGGTGACCAAGCGTCTGC
  ACACGTTAGAGGAAGTTAACAACAACGTGAGACTGCTCAGTGAGATGCTGCTTCATTACAGCCAGGAGGA
  CTCTTCGGACGGGGACAGAGAGCTGATGAAGGAGCTGTTTGATCAGTGTGAGAACAAGAGGCGGACTTTA
  TTTAAACTCGCCAGTGAGACTGAGGACAATGATAACAGTTTGGGGGACATCCTGCAAGCCAGTGACAACC
  TCTCCCGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGGTCATCAATGGCGAGGTGGCTACCTT
  AACCCTGCCTGACTCGGAAGGAAACAGTCAGTGCAGTAACCAAGGCACGCTCATCGACCTTGCGGAGCTG
  GACACGACCAACAGTTTGTCCTCCGTGTTGGCCCCAGCACCTACTCCACCCTCCTCAGGCATCCCAATCC
  TCCCTCCACCACCCCAGGCCTCAGGACCTCCACGGAGCCGCTCCTCTAGCCAGGCCGAGGCCACCCTGGG
  GCCCAGCAGCACAAGCAACGCCCTCTCCTGGCTGGACGAGGAGCTACTCTGCTTGGGCCTCGCCGACCCA
  GCCCCTAATGTTCCTCCCAAAGAGTCAGCTGGGAACAGCCAGTGGCACCTGCTCCAGAGGGAACAGTCCG
  ACCTGGACTTCTTCAGCCCCAGGCCGGGGACCGCTGCCTGTGGCGCCTCCGATGCTCCTCTGCTCCAGCC
  CTCAGCCCCCTCCTCAAGCAGCTCCCAAGCTCCACTGCCGCCTCCCTTCCCAGCTCCTGTGGTCCCAGCC
  AGTGTTCCTGCCCCCAGTGCGGGCTCCTCCTTGTTTTCTACTGGAGTGGCCCCAGCCTTGGCCCCAAAAG
  TTGAGCCCGCAGTCCCTGGGCACCATGGCTTGGCGTTGGGCAACAGCGCGCTGCACCACCTGGATGCCCT
  CGATCAGCTTCTAGAAGAGGCCAAAGTGACCTCGGGCTTGGTGAAACCCACTACCTCCCCTCTCATCCCC
  ACCACCACCCCAGCCAGGCCCCTCCTGCCCTTCTCCACGGGGCCCGGCAGCCCGCTCTTCCAGCCACTGA
  GTTTCCAGTCCCAGGGCAGCCCCCCGAAGGGGCCTGAGCTCTCCCTGGCCAGCATCCACGTGCCCCTGGA
  ATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTACGATAAAAACGGCTTCCGCATCCTCTTCCAC
  TTTGCCAAGGAGTGTCCCCCAGGACGACCTGACGTGCTGGTGGTGGTGGTGTCCATGCTGAACACGGCTC
  CCTTACCTGTCAAGAGCATCGTGCTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGTTGCAGCCACC
  TTCTGGGACAGAACTCTCTCCATTTAGCCCCATCCAGCCACCTGCAGCCATCACCCAGGTCATGTTGCTG
  GCCAATCCACTGAAGAAGCAGGTACTCAGCTTTCTGGGAAAAGCGTGCCTCCAGCCGTGGGGACAGGCCA
  TCCTACTGACTACCTCTTGCTTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230320 representing NM_001172704
  Red=Cloning site Green=Tags(s)
  
  MALPEEAKIKDAYHMLKRQGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQE
  ANKLIKSMVKEDEARIQKVTKRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTL
  FKLASETEDNDNSLGDILQASDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAEL
  DTTNSLSSVLAPAPTPPSSGIPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADP
  APNVPPKESAGNSQWHLLQREQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPA
  SVPAPSAGSSLFSTGVAPALAPKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIP
  TTTPARPLLPFSTGPGSPLFQPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFH
  FAKECPPGRPDVLVVVVSMLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLL
  ANPLKKQVLSFLGKACLQPWGQAILLTTSCLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172704
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172704.1, NM_001172704.2, NP_001166175.1
• RefSeq ORF: 1779
• Locus ID: 23163
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 63.4 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]