STAT2 (NM_198332) Human Tagged ORF Clone

CAT#: RC230538

STAT2 (Myc-DDK-tagged)-Human signal transducer and activator of transcription 2, 113kDa (STAT2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAT2
• Synonyms: IMD44; ISGF-3; P113; STAT113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230538 representing NM_198332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGTGGGAAATGCTGCAGAATCTTGACAGCCCCTTTCAGGATCAGCTGCACCAGCTTTACTCGC
  ACAGCCTCCTGCCTGTGGACATTCGACAGTACTTGGCTGTCTGGATTGAAGACCAGAACTGGCAGGAAGC
  TGCACTTGGGAGTGATGATTCCAAGGCTACCATGCTATTCTTCCACTTCTTGGATCAGCTGAACTATGAG
  TGTGGCCGTTGCAGCCAGGACCCAGAGTCCTTGTTGCTGCAGCACAATTTGCGGAAATTCTGCCGGGACA
  TTCAGGATCCTACCCAGTTGGCTGAGATGATCTTTAACCTCCTTCTGGAAGAAAAAAGAATTTTGATCCA
  GGCTCAGAGGGCCCAATTGGAACAAGGAGAGCCAGTTCTCGAAACACCTGTGGAGAGCCAGCAACATGAG
  ATTGAATCCCGGATCCTGGATTTAAGGGCTATGATGGAGAAGCTGGTAAAATCCATCAGCCAACTGAAAG
  ACCAGCAGGATGTCTTCTGCTTCCGATATAAGATCCAGGCCAAAGGGAAGACACCCTCTCTGGACCCCCA
  TCAGACCAAAGAGCAGAAGATTCTGCAGGAAACTCTCAATGAACTGGACAAAAGGAGAAAGGAGGTGCTG
  GATGCCTCCAAAGCACTGCTAGGCCGATTAACTACCCTAATCGAGCTACTGCTGCCAAAGTTGGAGGAGT
  GGAAGGCCCAGCAGCAAAAAGCCTGCATCAGAGCTCCCATTGACCACGGGTTGGAACAGCTGGAGACATG
  GTTCACAGCTGGAGCAAAGCTGTTGTTTCACCTGAGGCAGCTGCTGAAGGAGCTGAAGGGACTGAGTTGC
  CTGGTTAGCTATCAGGATGACCCTCTGACCAAAGGGGTGGACCTACGCAACGCCCAGGTCACAGAGTTGC
  TACAGCGTCTGCTCCACAGAGCCTTTGTGGTAGAAACCCAGCCCTGCATGCCCCAAACTCCCCATCGACC
  CCTCATCCTCAAGACTGGCAGCAAGTTCACCGTCCGAACAAGGCTGCTGGTGAGACTCCAGGAAGGCAAT
  GAGTCACTGACTGTGGAAGTCTCCATTGACAGGAATCCTCCTCAATTACAAGGCTTCCGGAAGTTCAACA
  TTCTGACTTCAAACCAGAAAACTTTGACCCCCGAGAAGGGGCAGAGTCAGGGTTTGATTTGGGACTTTGG
  TTACCTGACTCTGGTGGAGCAACGTTCAGGTGGTTCAGGAAAGGGCAGCAATAAGGGGCCACTAGGTGTG
  ACAGAGGAACTGCACATCATCAGCTTCACGGTCAAATATACCTACCAGGGTCTGAAGCAGGAGCTGAAAA
  CGGACACCCTCCCTGTGGTGATTATTTCCAACATGAACCAGCTCTCAATTGCCTGGGCTTCAGTTCTCTG
  GTTCAATTTGCTCAGCCCAAACCTTCAGAACCAGCAGTTCTTCTCCAACCCCCCCAAGGCCCCCTGGAGC
  TTGCTGGGCCCTGCTCTCAGTTGGCAGTTCTCCTCCTATGTTGGCCGAGGCCTCAACTCAGACCAGCTGA
  GCATGCTGAGAAACAAGCTGTTCGGGCAGAACTGTAGGACTGAGGATCCATTATTGTCCTGGGCTGACTT
  CACTAAGCGAGAGAGCCCTCCTGGCAAGTTACCATTCTGGACATGGCTGGACAAAATTCTGGAGTTGGTA
  CATGACCACCTGAAGGATCTCTGGAATGATGGACGCATCATGGGCTTTGTGAGTCGGAGCCAGGAGCGCC
  GGCTGCTGAAGAAGACCATGTCTGGCACCTTTCTACTGCGCTTCAGTGAATCGTCAGAAGGGGGCATTAC
  CTGCTCCTGGGTGGAGCACCAGGATGATGACAAGGTGCTCATCTACTCTGTGCAACCGTACACGAAGGAG
  GTGCTGCAGTCACTCCCGCTGACTGAAATCATCCGCCATTACCAGTTGCTCACTGAGGAGAATATACCTG
  AAAACCCACTGCGCTTCCTCTATCCCCGAATCCCCCGGGATGAAGCTTTTGGGTGCTACTACCAGGAGAA
  AGTTAATCTCCAGGAACGGAGGAAATACCTGAAACACAGGCTCATTGTGGTCTCTAATAGACAGGTGGAT
  GAACTGCAACAACCGCTGGAGCTTAAGCCAGAGCCAGAGCTGGAGTCATTAGAGCTGGAACTAGGGCTGG
  TGCCAGAGCCAGAGCTCAGCCTGGACTTAGAGCCACTGCTGAAGGCAGGGCTGGATCTGGGGCCAGAGCT
  AGAGTCTGTGCTGGAGTCCACTCTGGAGCCTGTGATAGAGCCCACACTATGCATGGTATCACAAACAGTG
  CCAGAGCCAGACCAAGGACCTGTATCACAGCCAGTGCCAGAGCCAGATTTGCCCTGTGATCTGAGACATT
  TGAACACTGAGCCAATGGAAATCTTCAGAAACTGTGTAAAGATTGAAGAAATCATGCCGAATGGTGACCC
  ACTGTTGGCTGGCCAGAACACCGTGGATGAGGTTTACGTCTCCCGCCCCAGCCACTTCTACACTGATGGA
  CCCTTGATGCCTTCTGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230538 representing NM_198332
  Red=Cloning site Green=Tags(s)
  
  MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYE
  CGRCSQDPESLLLQHNLRKFCRDIQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHE
  IESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVL
  DASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSC
  LVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGN
  ESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGV
  TEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWS
  LLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELV
  HDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKE
  VLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVD
  ELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTV
  PEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDG
  PLMPSDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198332
• ORF Size: 2541 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198332.1, NP_938146.1
• RefSeq ORF: 2544 bp
• Locus ID: 6773
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway
• MW: 97.9 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44. [provided by RefSeq, Aug 2020]'