AFF2 (NM_001170628) Human Tagged ORF Clone

CAT#: RC230593

AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFF2
• Synonyms: FMR2; FMR2P; FRAXE; MRX2; OX19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230593 representing NM_001170628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCAAAAGGAGGCACCAAGCATTTCCGTCCTTCTTCAAGATGAAGGTAAGCCTTCCCAGTGATC
  CAAGCTGTGTTGAAGAAATCTTGCGGGAATCGCAGCATCTGACCCCAGGATTCACCTTACAAAAGTGGAA
  TGACCCAACCACCAGAGCTTCTACAAAGTCAGTGTCTTTCAAATCGATGCTTGAGGATGACCTGAAGCTG
  AGCAGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACC
  AGGCTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACC
  TGCAGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGAC
  ACTGAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCAC
  CCTCAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTG
  TGGCCAAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAG
  ATGAAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTA
  GGGAGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAAC
  AACTAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGC
  ACTGACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGC
  TTCATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACC
  TAACATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAA
  TTCATTGAAACAGATTCATCTACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCC
  TGCCTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCT
  CAGCACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCA
  CCTATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGG
  TGAAGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCC
  AGACCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCT
  AAGGGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGG
  AGGCCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAG
  AGAAAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCA
  CTGCCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACA
  TCGCCATGACTGGACAAATCACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAA
  AGGGATATCGGTAAATGAGGGAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACT
  GCTATTGCCACTGCTACTGTCACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCA
  CGGCCACCACCACAACTACTACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGA
  TAGCAGTCACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGG
  AGACCCAAGCTCACTTTTGATGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGA
  AGCACAAAGCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTT
  CACTGAATGTGGCAATGCCATGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAG
  ACTGTGGAGCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACA
  AAAAGCTAGCAGTACTATGCTACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGA
  CCATGCTATGAAGTACTCCAGATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATA
  CCCTCTCCATGGGTAAGCAATGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCA
  ACGCAATGGGGAACTGTAACAATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCA
  CGTCAACATCACTAGCAATGTGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAA
  AACAAAGAATTCTTTGGTGATCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATC
  TTGTCCGCTACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230593 representing NM_001170628
  Red=Cloning site Green=Tags(s)
  
  MKFKRRHQAFPSFFKMKVSLPSDPSCVEEILRESQHLTPGFTLQKWNDPTTRASTKSVSFKSMLEDDLKL
  SSDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSD
  TESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQ
  MKVKTNASQVPAEPKERPLLSLIREKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTS
  TDEFTWPKPNITSSTPKEKESVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSRE
  FIETDSSTSDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFS
  PIPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAP
  KGKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSP
  LPEDPPRRRNVSGNNGPFGQDKNIAMTGQITSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNT
  AIATATVTATAIVTTTVTATATATATTTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVR
  RPKLTFDDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSE
  TVELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQI
  PSPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTRE
  NKEFFGDLDTLMGPLTQHSSMTNLVRYVRQGLCWLRIDAHLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170628
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170628.1, NP_001164099.1
• RefSeq ORF: 2859 bp
• Locus ID: 2334
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• MW: 105.6 kDa
• Gene Summary: 'This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016]'