TJP2 (NM_001170414) Human Tagged ORF Clone

CAT#: RC230621

TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TJP2
• Synonyms: C9DUPq21.11; DFNA51; DUP9q21.11; PFIC4; X104; ZO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230621 representing NM_001170414
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCTGATATGGGAACAGTACACTGTGACCCTACAAAAGGATTCCAAAAGAGGATTTGGAATTG
  CAGTGTCCGGAGGCAGAGACAACCCCCACTTTGAAAATGGAGAAACGTCAATTGTCATTTCTGATGTGCT
  CCCGGGTGGGCCTGCTGATGGGCTGCTCCAAGAAAATGACAGAGTGGTCATGGTCAATGGCACCCCCATG
  GAGGATGTGCTTCATTCGTTTGCAGTTCAGCAGCTCAGAAAAAGTGGGAAGGTCGCTGCTATTGTGGTCA
  AGAGGCCCCGGAAGGTCCAGGTGGCCGCACTTCAGGCCAGCCCTCCCCTGGATCAGGATGACCGGGCTTT
  TGAGGTGATGGACGAGTTTGATGGCAGAAGTTTCCGGAGTGGCTACAGCGAGAGGAGCCGGCTGAACAGC
  CATGGGGGGCGCAGCCGCAGCTGGGAGGACAGCCCGGAAAGGGGGCGTCCCCATGAGCGGGCCCGGAGCC
  GGGAGCGGGACCTCAGCCGGGACCGGAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCAAGACCATGC
  GCGCACCCGAGACCGCAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCACGACTTTGGGCCATCCCGG
  GACCGGGACCGTGACCGCAGCCGCGGCCGGAGCATTGACCAGGACTACGAGCGAGCCTATCACCGGGCCT
  ACGACCCAGACTACGAGCGGGCCTACAGCCCGGAGTACAGGCGCGGGGCCCGCCACGATGCCCGCTCTCG
  GGGACCCCGAAGCCGCAGCCGCGAGCACCCGCACTCACGGAGCCCCAGCCCCGAGCCTAGGGGGCGGCCG
  GGGCCCATCGGGGTCCTCCTGATGAAAAGCAGAGCGAACGAAGAGTATGGTCTCCGGCTTGGGAGTCAGA
  TCTTCGTAAAGGAAATGACCCGAACGGGTCTGGCAACTAAAGATGGCAACCTTCACGAAGGAGACATAAT
  TCTCAAGATCAATGGGACTGTAACTGAGAACATGTCTTTAACGGATGCTCGAAAATTGATAGAAAAGTCA
  AGAGGAAAACTACAGCTAGTGGTGTTGAGAGACAGCCAGCAGACCCTCATCAACATCCCGTCATTAAATG
  ACAGTGACTCAGAAATAGAAGATATTTCAGAAATAGAGTCAAACCGATCATTTTCTCCAGAGGAGAGACG
  TCATCAGTATTCTGATTATGATTATCATTCCTCAAGTGAGAAGCTGAAGGAAAGGCCAAGTTCCAGAGAG
  GACACGCCGAGCAGATTGTCCAGGATGGGTGCGACACCCACTCCCTTTAAGTCCACAGGGGATATTGCAG
  GCACAGTTGTCCCAGAGACCAACAAGGAACCCAGATACCAAGAGGACCCCCCAGCTCCTCAACCAAAAGC
  AGCCCCGAGAACTTTTCTTCGTCCTAGTCCTGAAGATGAAGCAATATATGGCCCTAATACCAAAATGGTA
  AGGTTCAAGAAGGGAGACAGCGTGGGCCTCCGGTTGGCTGGTGGCAATGATGTCGGGATATTTGTTGCTG
  GCATTCAAGAAGGGACCTCGGCGGAGCAGGAGGGCCTTCAAGAAGGAGACCAGATTCTGAAGGTGAACAC
  ACAGGATTTCAGAGGATTAGTGCGGGAGGATGCCGTTCTCTACCTGTTAGAAATCCCTAAAGGTGAAATG
  GTGACCATTTTAGCTCAGAGCCGAGCCGATGTGTATAGAGACATCCTGGCTTGTGGCAGAGGGGATTCGT
  TTTTTATAAGAAGCCACTTTGAATGTGAGAAGGAAACTCCACAGAGCCTGGCCTTCACCAGAGGGGAGGT
  CTTCCGAGTGGTAGACACACTGTATGACGGCAAGCTGGGCAACTGGCTGGCTGTGAGGATTGGGAACGAG
  TTGGAGAAAGGCTTAATCCCCAACAAGAGCAGAGCTGAACAAATGGCCAGTGTTCAAAATGCCCAGAGAG
  ACAACGCTGGGGACCGGGCAGATTTCTGGAGAATGCGTGGCCAGAGGTCTGGGGTGAAGAAGAACCTGAG
  GAAAAGTCGGGAAGACCTCACAGCTGTTGTGTCTGTCAGCACCAAGTTCCCAGCTTATGAGAGGGTTTTG
  CTGCGAGAAGCTGGTTTCAAGAGACCTGTGGTCTTATTCGGCCCCATAGCTGATATAGCAATGGAAAAAT
  TGGCTAATGAGTTACCTGACTGGTTTCAAACTGCTAAAACGGAACCAAAAGATGCAGGATCTGAGAAATC
  CACTGGAGTGGTCCGGTTAAATACCGTGAGGCAAATTATTGAACAGGATAAGCATGCACTACTGGATGTG
  ACTCCGAAAGCTGTGGACCTGTTGAATTACACCCAGTGGTTCCCAATTGTGATTTTTTTCAACCCAGACT
  CCAGACAAGGTGTCAAAACCATGAGACAAAGGTTAAATCCAACGTCCAACAAAAGTTCTCGAAAGTTATT
  TGATCAAGCCAACAAGCTTAAAAAAACGTGTGCACACCTTTTTACAGCTACAATCAACCTAAATTCAGCC
  AATGATAGCTGGTTTGGCAGCTTAAAGGACACTATTCAGCATCAGCAAGGAGAAGCGGTTTGGGTCTCTG
  AAGGAAAGATGGAAGGGATGGATGATGACCCCGAAGACCGCATGTCCTACTTAACCGCCATGGGCGCGGA
  CTATCTGAGTTGCGACAGCCGCCTCATCAGTGACTTTGAAGACACGGACGGTGAAGGAGGCGCCTACACT
  GACAATGAGCTGGATGAGCCAGCCGAGGAGCCGCTGGTGTCGTCCATCACCCGCTCCTCGGAGCCGGTGC
  AGCACGAGGAGATCGAAATTGCCCAGAAGCATCCTGATATCTATGCAGTTCCAATCAAAACGCACAAGCC
  AGACCCTGGCACGCCCCAGCACACGAGTTCCAGACCCCCTGAGCCACAGAAAGCTCCTTCCAGACCTTAT
  CAGGATACCAGAGGAAGTTATGGCAGTGATGCCGAGGAGGAGGAGTACCGCCAGCAGCTGTCAGAACACT
  CCAAGCGCGGTTACTATGGCCAGTCTGCCCGATACCGGGACACAGAATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230621 representing NM_001170414
  Red=Cloning site Green=Tags(s)
  
  MEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPM
  EDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNS
  HGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSR
  DRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREHPHSRSPSPEPRGRP
  GPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKS
  RGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERRHQYSDYDYHSSSEKLKERPSSRE
  DTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKAAPRTFLRPSPEDEAIYGPNTKMV
  RFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGEM
  VTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGNWLAVRIGNE
  LEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLRKSREDLTAVVSVSTKFPAYERVL
  LREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKSTGVVRLNTVRQIIEQDKHALLDV
  TPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLFDQANKLKKTCAHLFTATINLNSA
  NDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYT
  DNELDEPAEEPLVSSITRSSEPVQHEEIEIAQKHPDIYAVPIKTHKPDPGTPQHTSSRPPEPQKAPSRPY
  QDTRGSYGSDAEEEEYRQQLSEHSKRGYYGQSARYRDTEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170414
• ORF Size: 3060 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170414.1, NM_001170414.2, NP_001163885.1
• RefSeq Size: 4129
• RefSeq ORF: 3063
• Locus ID: 9414
• Protein Pathways: Tight junction, Vibrio cholerae infection
• MW: 115.2 kDa
• Gene Summary: This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]