IGSF1 (NM_001170961) Human Tagged ORF Clone

CAT#: RC230705

IGSF1 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 1 (IGSF1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGSF1
• Synonyms: CHTE; IGCD1; IGDC1; INHBP; p120; PGSF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230705 representing NM_001170961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGGACAGACCAGGGGAGGGGGCCACCATGCTGAAGACATTCACTGTTTTGCTCTTTTGCATTC
  GGATGAGTCTGGGTATGACATCGATAGTGATGGACCCTCAACCGGAGTTGTGGATAGAGTCCAACTACCC
  CCAGGCCCCTTGGGAGAACATCACGCTTTGGTGCCGAAGCCCCTCTCGGATATCAAGCAAGTTCCTGCTG
  CTGAAGGATAAGACACAGATGACCTGGATCCGCCCTTCCCACAAGACCTTCCAAGTTTCATTCCTTATAG
  GTGCCCTTACTGAGTCCAATGCAGGTCTTTACCGGTGCTGCTACTGGAAGGAGACAGGCTGGTCAAAGCC
  CAGTAAAGTTCTAGAGTTGGAGGCACCAGGCCAACTGCCCAAGCCCATCTTCTGGATTCAGGCTGAGACC
  CCCGCTCTTCCTGGGTGTAATGTTAACATCCTCTGCCATGGCTGGCTGCAGGATTTGGTATTCATGCTGT
  TTAAAGAGGGATATGCAGAGCCTGTGGATTACCAAGTCCCAACTGGGACAATGGCCATATTCTCCATTGA
  CAACCTGACACCTGAGGATGAAGGGGTTTACATCTGCCGCACTCATATCCAGATGCTCCCCACCCTGTGG
  TCAGAGCCCAGCAACCCCCTGAAGCTGGTTGTAGCAGGACTCTACCCCAAACCAACTTTGACAGCCCATC
  CTGGGCCCATCATGGCACCTGGAGAAAGCCTGAATCTCAGGTGCCAAGGGCCAATCTATGGAATGACCTT
  TGCTCTAATGAGGGTTGAAGACTTGGAGAAGTCCTTTTACCACAAGAAGACAATAAAAAATGAGGCAAAT
  TTCTTCTTCCAGTCTTTGAAGATCCAAGATACTGGACATTACCTCTGTTTTTACTATGACGCATCATATA
  GAGGTTCACTCCTTAGTGATGTCCTGAAAATCTGGGTGACTGACACTTTCCCCAAGACCTGGCTACTTGC
  TCGGCCCAGTGCTGTGGTCCAAATGGGTCAGAATGTGAGCCTACGGTGTCGAGGACCAGTGGATGGAGTG
  GGTCTTGCACTCTATAAGAAAGGAGAAGACAAACCACTTCAATTTTTGGATGCCACCAGCATCGATGACA
  ACACATCATTCTTCCTCAACAATGTAACCTACAGTGATACTGGCATCTATAGCTGCCACTATCTTCTCAC
  CTGGAAGACCTCCATTAGGATGCCATCACACAACACTGTGGAGCTTATGGTTGTAGATAAGCCCCCCAAA
  CCCTCCCTGTCAGCTTGGCCAAGCACTGTGTTCAAGCTAGGAAAGGCCATCACCCTTCAGTGCCGAGTAT
  CTCATCCAGTACTGGAATTTTCTCTGGAATGGGAAGAAAGAGAAACATTCCAAAAATTCTCAGTAAACGG
  AGACTTCATCATCAGTAATGTTGACGGGAAAGGCACAGGGACCTACAGTTGCAGCTATCGCGTAGAGACA
  CATCCTAACATCTGGTCACATCGCAGTGAGCCCCTGAAGCTGATGGGGCCAGCAGGCTATCTCACCTGGA
  ATTACGTTCTGAATGAAGCTATCAGGTTGTCTCTAATCATGCAGCTTGTTGCCTTGCTGTTGGTAGTGCT
  GTGGATAAGGTGGAAGTGTCGGAGACTCAGAATCAGAGAAGCCTGGTTGCTGGGAACAGCTCAAGGGGTC
  ACCATGCTCTTCATAGTCACGGCCCTTCTCTGCTGTGCAATTTCTTTTGCAGGACTGTGCAATGGGGTAT
  TGATAGAAGAGACTGAAATAGTCATGCCAACCCCTAAGCCTGAGCTGTGGGCAGAGACCAACTTTCCTCT
  GGCCCCGTGGAAGAACTTAACCCTCTGGTGCAGAAGCCCTTCTGGCTCAACTAAGGAGTTTGTGTTGCTG
  AAGGATGGGACCGGGTGGATCGCCACTCGCCCGGCCTCAGAGCAGGTCCGGGCTGCCTTCCCCCTTGGCG
  CCCTGACCCAGAGCCACACCGGGAGCTACCACTGCCATTCATGGGAGGAGATGGCTGTATCGGAGCCCAG
  TGAGGCACTTGAGCTGGTGGGGACAGACATCCTCCCCAAACCTGTCATTTCTGCTTCCCCCACAATCCGG
  GGCCAGGAACTACAACTCCGGTGCAAAGGATGGCTGGCAGGCATGGGGTTTGCTCTGTATAAGGAGGGAG
  AGCAAGAACCTGTCCAGCAACTTGGTGCTGTTGGAAGAGAAGCCTTCTTTACAATCCAGAGAATGGAGGA
  TAAAGACGAAGGCAATTACAGCTGCCGCACTCACACTGAAAAACGCCCCTTCAAGTGGTCTGAGCCCAGT
  GAGCCGCTGGAGCTTGTCATAAAAGAAATGTACCCTAAGCCCTTCTTCAAGACATGGGCCAGCCCTGTGG
  TCACCCCTGGTGCCCGAGTGACTTTCAATTGCTCCACCCCCCACCAGCATATGAGCTTTATTCTTTACAA
  AGATGGAAGTGAAATAGCATCCAGTGACAGGTCCTGGGCAAGTCCGGGGGCCAGTGCAGCTCACTTTCTA
  ATCATTTCGGTGGGCATTGGTGATGGAGGGAATTACAGCTGCCGATATTATGACTTTTCTATCTGGTCTG
  AGCCCAGCGACCCTGTGGAGCTCGTGGTGACAGAATTCTACCCCAAACCCACTCTCCTGGCACAGCCAGG
  TCCTGTGGTGTTTCCTGGGAAGAGTGTGATCCTGCGCTGCCAAGGGACTTTCCAGGGCATGAGGTTCGCC
  CTCTTGCAGGAGGGAGCCCATGTTCCCTTACAGTTTCGGAGTGTCTCAGGGAACTCAGCTGACTTCCTTC
  TCCACACTGTTGGAGCAGAGGACTCTGGGAACTATAGCTGTATCTACTATGAGACAACCATGTCAAACAG
  GGGGTCATATCTCAGTATGCCCCTTATGATCTGGGTGACTGACACATTCCCTAAGCCATGGTTGTTTGCT
  GAGCCCAGTTCTGTGGTTCCCATGGGGCAGAATGTTACTCTCTGGTGCCGAGGGCCGGTCCATGGAGTAG
  GATACATTCTGCACAAAGAAGGAGAAGCCACTTCAATGCAGCTCTGGGGATCCACCAGTAATGACGGGGC
  ATTCCCCATCACCAATATATCTGGTACTAGCATGGGGCGTTACAGCTGCTGCTACCACCCTGACTGGACC
  AGTTCTATCAAGATACAACCTAGCAACACCCTGGAACTCCTAGTCACAGGCTTACTCCCCAAACCCAGCC
  TATTAGCCCAGCCTGGTCCCATGGTGGCCCCTGGCGAAAATATGACTCTTCAGTGTCAAGGGGAACTGCC
  AGACTCAACATTTGTCCTGTTGAAGGAGGGGGCTCAGGAGCCTTTAGAGCAACAGAGGCCAAGTGGGTAC
  AGGGCTGACTTCTGGATGCCAGCAGTGAGAGGTGAAGACTCTGGGATCTATAGCTGTGTTTATTATTTGG
  ACTCTACTCCCTTTGCAGCTTCAAATCACAGTGACTCCCTGGAGATCTGGGTGACTGATAAGCCCCCTAA
  ACCCTCTCTGTCAGCCTGGCCCAGCACCATGTTCAAGTTAGGGAAGGACATCACCCTTCAGTGCCGAGGA
  CCCCTGCCAGGTGTTGAATTTGTCCTAGAACATGATGGAGAAGAAGCACCTCAGCAGTTTTCAGAGGATG
  GAGACTTTGTCATCAACAACGTAGAAGGAAAAGGCATTGGAAACTACAGCTGCAGCTACCGCCTCCAGGC
  CTACCCTGATATCTGGTCAGAGCCTAGTGATCCCCTGGAGCTGGTGGGGGCAGCAGGGCCTGTTGCTCAG
  GAGTGCACTGTAGGGAACATTGTCCGAAGTAGCCTAATCGTGGTGGTTGTTGTAGCCTTGGGGGTAGTGC
  TAGCCATAGAGTGGAAGAAGTGGCCTCGACTGCGAACCAGAGGCTCAGAGACAGACGGAAGAGACCAGAC
  CATTGCCCTTGAAGAGTGTAACCAAGAAGGAGAACCAGGCACCCCTGCCAATTCTCCTTCATCAACCTCT
  CAGAGAATCTCTGTGGAACTGCCCGTTCCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230705 representing NM_001170961
  Red=Cloning site Green=Tags(s)
  
  MTLDRPGEGATMLKTFTVLLFCIRMSLGMTSIVMDPQPELWIESNYPQAPWENITLWCRSPSRISSKFLL
  LKDKTQMTWIRPSHKTFQVSFLIGALTESNAGLYRCCYWKETGWSKPSKVLELEAPGQLPKPIFWIQAET
  PALPGCNVNILCHGWLQDLVFMLFKEGYAEPVDYQVPTGTMAIFSIDNLTPEDEGVYICRTHIQMLPTLW
  SEPSNPLKLVVAGLYPKPTLTAHPGPIMAPGESLNLRCQGPIYGMTFALMRVEDLEKSFYHKKTIKNEAN
  FFFQSLKIQDTGHYLCFYYDASYRGSLLSDVLKIWVTDTFPKTWLLARPSAVVQMGQNVSLRCRGPVDGV
  GLALYKKGEDKPLQFLDATSIDDNTSFFLNNVTYSDTGIYSCHYLLTWKTSIRMPSHNTVELMVVDKPPK
  PSLSAWPSTVFKLGKAITLQCRVSHPVLEFSLEWEERETFQKFSVNGDFIISNVDGKGTGTYSCSYRVET
  HPNIWSHRSEPLKLMGPAGYLTWNYVLNEAIRLSLIMQLVALLLVVLWIRWKCRRLRIREAWLLGTAQGV
  TMLFIVTALLCCAISFAGLCNGVLIEETEIVMPTPKPELWAETNFPLAPWKNLTLWCRSPSGSTKEFVLL
  KDGTGWIATRPASEQVRAAFPLGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASPTIR
  GQELQLRCKGWLAGMGFALYKEGEQEPVQQLGAVGREAFFTIQRMEDKDEGNYSCRTHTEKRPFKWSEPS
  EPLELVIKEMYPKPFFKTWASPVVTPGARVTFNCSTPHQHMSFILYKDGSEIASSDRSWASPGASAAHFL
  IISVGIGDGGNYSCRYYDFSIWSEPSDPVELVVTEFYPKPTLLAQPGPVVFPGKSVILRCQGTFQGMRFA
  LLQEGAHVPLQFRSVSGNSADFLLHTVGAEDSGNYSCIYYETTMSNRGSYLSMPLMIWVTDTFPKPWLFA
  EPSSVVPMGQNVTLWCRGPVHGVGYILHKEGEATSMQLWGSTSNDGAFPITNISGTSMGRYSCCYHPDWT
  SSIKIQPSNTLELLVTGLLPKPSLLAQPGPMVAPGENMTLQCQGELPDSTFVLLKEGAQEPLEQQRPSGY
  RADFWMPAVRGEDSGIYSCVYYLDSTPFAASNHSDSLEIWVTDKPPKPSLSAWPSTMFKLGKDITLQCRG
  PLPGVEFVLEHDGEEAPQQFSEDGDFVINNVEGKGIGNYSCSYRLQAYPDIWSEPSDPLELVGAAGPVAQ
  ECTVGNIVRSSLIVVVVVALGVVLAIEWKKWPRLRTRGSETDGRDQTIALEECNQEGEPGTPANSPSSTS
  QRISVELPVPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170961
• ORF Size: 4023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170961.1, NP_001164432.1
• RefSeq ORF: 4026 bp
• Locus ID: 3547
• Cytogenetics: Xq26.1
• Protein Families: Transmembrane
• MW: 149.9 kDa
• Gene Summary: 'This gene encodes a member of the immunoglobulin-like domain-containing superfamily. Proteins in this superfamily contain varying numbers of immunoglobulin-like domains and are thought to participate in the regulation of interactions between cells. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]'