Neogenin (NEO1) (NM_001172623) Human Tagged ORF Clone

CAT#: RC230713

NEO1 (Myc-DDK-tagged)-Human neogenin 1 (NEO1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEO1
• Synonyms: IGDCC2; NGN; NTN1R2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230713 representing NM_001172623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGCGGGGAGCCCGGCGACTCCTCAGCACCCCCTCCTTCTGGCTCTACTGCCTGCTGCTGC
  TCGGGCGCCGGGCGCCGGGCGCCGCGGCCGCCAGGAGCGGCTCCGCGCCGCAGTCCCCAGGAGCCAGCAT
  TCGAACGTTCACTCCATTTTATTTTCTGGTGGAGCCGGTGGATACACTCTCAGTTAGAGGCTCTTCTGTT
  ATATTAAACTGTTCAGCATATTCTGAGCCTTCTCCAAAAATTGAATGGAAAAAAGATGGAACTTTTTTAA
  ACTTAGTATCAGATGATCGACGCCAGCTTCTCCCGGATGGATCTTTATTTATCAGCAATGTGGTGCATTC
  CAAACACAATAAACCTGATGAAGGTTATTATCAGTGTGTGGCCACTGTTGAGAGTCTTGGAACTATTATC
  AGTAGAACAGCGAAGCTCATAGTAGCAGGTCTTCCAAGATTTACCAGCCAACCAGAACCTTCCTCAGTTT
  ATGCTGGGAACAATGCAATTCTGAATTGTGAAGTTAATGCAGATTTGGTCCCATTTGTGAGGTGGGAACA
  GAACAGACAACCCCTTCTTCTGGATGATAGAGTTATCAAACTTCCAAGTGGAATGCTGGTTATCAGCAAT
  GCAACTGAAGGAGATGGCGGGCTTTATCGCTGCGTAGTGGAAAGTGGTGGGCCACCAAAGTATAGTGATG
  AAGTTGAATTGAAGGTTCTTCCAGATCCTGAGGTGATATCAGACTTGGTATTTTTGAAACAGCCTTCTCC
  CTTAGTCAGAGTCATTGGTCAGGATGTAGTGTTGCCATGTGTTGCTTCAGGACTTCCTACTCCAACCATT
  AAATGGATGAAAAATGAGGAGGCACTTGACACAGAAAGCTCTGAAAGATTGGTATTGCTGGCAGGTGGTA
  GCCTGGAGATCAGTGATGTTACTGAGGATGATGCTGGGACTTATTTTTGTATAGCTGATAATGGAAATGA
  GACAATTGAAGCTCAAGCAGAGCTTACAGTGCAAGCTCAACCTGAATTCCTGAAGCAGCCTACTAATATA
  TATGCTCACGAATCTATGGATATTGTATTTGAATGTGAAGTGACTGGAAAACCAACTCCAACTGTGAAGT
  GGGTCAAAAATGGGGATATGGTTATCCCAAGTGATTATTTTAAGATTGTAAAGGAACATAATCTTCAAGT
  TTTGGGTCTGGTGAAATCAGATGAAGGGTTCTATCAGTGCATTGCTGAAAATGATGTTGGAAATGCACAA
  GCTGGAGCCCAACTGATAATCCTTGAACATGCACCAGCCACAACGGGACCACTGCCTTCAGCTCCTCGGG
  ATGTCGTGGCCTCCCTGGTCTCTACCCGCTTCATCAAATTGACGTGGCGGACACCTGCATCAGATCCTCA
  CGGAGACAACCTTACCTACTCTGTGTTCTACACCAAGGAAGGGATTGCTAGGGAACGTGTTGAGAATACC
  AGTCACCCAGGAGAGATGCAAGTAACCATTCAAAACCTAATGCCAGCGACCGTGTACATCTTTAGAGTTA
  TGGCTCAAAATAAGCATGGCTCAGGAGAGAGTTCAGCTCCACTGCGAGTAGAAACACAACCTGAGGTTCA
  GCTCCCTGGCCCAGCACCTAACCTTCGTGCATATGCAGCTTCGCCTACCTCCATCACTGTTACGTGGGAA
  ACACCAGTGTCTGGCAATGGGGAAATTCAGAATTATAAATTGTACTACATGGAAAAGGGGACTGATAAAG
  AACAGGATGTTGATGTTTCAAGTCACTCTTACACCATTAATGGGTTGAAAAAATATACAGAGTATAGTTT
  CCGAGTGGTGGCCTACAATAAACATGGTCCTGGAGTTTCCACACCAGATGTTGCTGTTCGAACATTGTCA
  GATGTTCCCAGTGCTGCTCCTCAGAATCTGTCCTTGGAAGTGAGAAATTCAAAGAGTATTATGATTCACT
  GGCAGCCACCTGCTCCAGCCACACAAAATGGGCAGATTACTGGCTACAAGATTCGCTACCGAAAGGCCTC
  CCGAAAGAGTGATGTCACTGAGACCTTGGTAAGCGGGACACAGCTGTCTCAGCTGATTGAAGGTCTTGAT
  CGGGGGACTGAGTATAATTTCCGAGTGGCTGCTCTAACAATCAATGGTACAGGCCCGGCAACTGACTGGC
  TGTCTGCTGAAACTTTTGAAAGTGACCTAGATGAAACTCGTGTTCCTGAAGTGCCTAGCTCTCTTCACGT
  ACGCCCGCTCGTTACTAGCATCGTAGTGAGCTGGACTCCTCCAGAGAATCAGAACATTGTGGTCAGAGGT
  TACGCCATTGGTTATGGCATTGGCAGCCCTCATGCCCAGACCATCAAAGTGGACTATAAACAGCGCTATT
  ACACCATTGAAAATCTGGATCCCAGCTCTCACTATGTGATTACCCTGAAAGCATTTAATAACGTGGGTGA
  AGGCATCCCCCTGTATGAGAGTGCTGTGACCAGGCCTCACACAGACACTTCTGAAGTTGATTTATTTGTT
  ATTAATGCTCCATACACTCCAGTGCCAGATCCCACTCCCATGATGCCACCAGTGGGAGTTCAGGCTTCCA
  TTCTGAGTCATGACACCATCAGGATTACGTGGGCAGACAACTCGCTGCCCAAGCACCAGAAGATTACAGA
  CTCCCGATACTACACCGTCCGATGGAAAACCAACATCCCAGCAAACACCAAGTACAAGAATGCAAATGCA
  ACCACTTTGAGTTATTTGGTGACTGGTTTAAAGCCGAATACACTCTATGAATTCTCTGTGATGGTGACCA
  AAGGTCGAAGATCAAGTACATGGAGTATGACAGCCCATGGGACCACCTTTGAATTAGTTCCGACTTCTCC
  ACCCAAGGATGTGACTGTTGTGAGTAAAGAGGGGAAACCTAAGACCATAATTGTGAATTGGCAGCCTCCC
  TCCGAAGCCAATGGCAAAATTACAGGTTACATCATATATTACAGTACAGATGTGAATGCAGAGATACATG
  ACTGGGTTATTGAGCCTGTTGTGGGAAACAGACTGACTCACCAGATACAAGAGTTAACTCTTGACACACC
  ATACTACTTCAAAATCCAGGCACGGAACTCAAAGGGCATGGGACCCATGTCTGAAGCTGTCCAATTCAGA
  ACACCTAAAGCGGACTCCTCTGATAAAATGCCTAATGATCAAGCCTCAGGGTCTGGAGGGAAAGGAAGCC
  GGCTGCCAGACCTAGGATCCGACTACAAACCTCCAATGAGCGGCAGTAACAGCCCTCATGGGAGCCCCAC
  CTCTCCTCTGGACAGTAATATGCTGCTGGTCATAATTGTTTCTGTTGGCGTCATCACCATCGTGGTGGTT
  GTGATTATCGCTGTCTTTTGTACCCGTCGTACCACCTCTCACCAGAAAAAGAAACGAGCTGCCTGCAAAT
  CAGTGAATGGCTCTCATAAGTACAAAGGGAATTCCAAAGATGTGAAACCTCCAGATCTCTGGATCCATCA
  TGAGAGACTGGAGCTGAAACCCATTGATAAGTCTCCAGACCCAAACCCCATCATGACTGATACTCCAATT
  CCTCGCAACTCTCAAGATATCACACCAGTTGACAACTCCATGGACAGCAATATCCATCAAAGGCGAAATT
  CATACAGAGGGCATGAGTCAGAGGACAGCATGTCTACACTGGCTGGAAGGCGAGGAATGAGACCAAAAAT
  GATGATGCCCTTTGACTCCCAGCCACCCCAGCAATCCGTTCGAAATACCCCCAGCACTGACACCATGCCA
  GCCTCTTCGTCTCAAACATGCTGCACTGATCACCAGGACCCTGAAGGTGCTACCAGCTCCTCTTACTTGG
  CCAGCTCCCAAGAGGAAGATTCAGGCCAGAGTCTTCCCACTGCCCATGTTCGCCCTTCCCACCCATTGAA
  GAGCTTCGCCGTGCCAGCAATCCCGCCTCCAGGACCTCCCACCTATGATCCTGCATTGCCAAGCACACCA
  TTACTGTCCCAGCAAGCTCTGAACCATCACATTCACTCAGTGAAGACAGCCTCCATCGGGACTCTAGGAA
  GGAGCCGGCCTCCTATGCCAGTGGTTGTTCCCAGTGCCCCTGAAGTGCAGGAGACCACAAGGATGTTGGA
  AGACTCCGAGAGTAGCTATGAACCAGATGAGCTGACCAAAGAGATGGCCCACCTGGAAGGACTAATGAAG
  GACCTAAACGCTATCACAACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230713 representing NM_001172623
  Red=Cloning site Green=Tags(s)
  
  MAAERGARRLLSTPSFWLYCLLLLGRRAPGAAAARSGSAPQSPGASIRTFTPFYFLVEPVDTLSVRGSSV
  ILNCSAYSEPSPKIEWKKDGTFLNLVSDDRRQLLPDGSLFISNVVHSKHNKPDEGYYQCVATVESLGTII
  SRTAKLIVAGLPRFTSQPEPSSVYAGNNAILNCEVNADLVPFVRWEQNRQPLLLDDRVIKLPSGMLVISN
  ATEGDGGLYRCVVESGGPPKYSDEVELKVLPDPEVISDLVFLKQPSPLVRVIGQDVVLPCVASGLPTPTI
  KWMKNEEALDTESSERLVLLAGGSLEISDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNI
  YAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQ
  AGAQLIILEHAPATTGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENT
  SHPGEMQVTIQNLMPATVYIFRVMAQNKHGSGESSAPLRVETQPEVQLPGPAPNLRAYAASPTSITVTWE
  TPVSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSYTINGLKKYTEYSFRVVAYNKHGPGVSTPDVAVRTLS
  DVPSAAPQNLSLEVRNSKSIMIHWQPPAPATQNGQITGYKIRYRKASRKSDVTETLVSGTQLSQLIEGLD
  RGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRG
  YAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFV
  INAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANA
  TTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPKTIIVNWQPP
  SEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFR
  TPKADSSDKMPNDQASGSGGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVV
  VIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPI
  PRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMP
  ASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPPGPPTYDPALPSTP
  LLSQQALNHHIHSVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMK
  DLNAITTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172623
• ORF Size: 4224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172623.1, NP_001166094.1
• RefSeq ORF: 4227 bp
• Locus ID: 4756
• Cytogenetics: 15q24.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 154.8 kDa
• Gene Summary: 'This gene encodes a cell surface protein that is a member of the immunoglobulin superfamily. The encoded protein consists of four N-terminal immunoglobulin-like domains, six fibronectin type III domains, a transmembrane domain and a C-terminal internal domain that shares homology with the tumor suppressor candidate gene DCC. This protein may be involved in cell growth and differentiation and in cell-cell adhesion. Defects in this gene are associated with cell proliferation in certain cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]'