ECHDC2 (NM_001198962) Human Tagged ORF Clone

CAT#: RC231091

ECHDC2 (Myc-DDK-tagged)-Human enoyl CoA hydratase domain containing 2 (ECHDC2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY434090) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231091 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ECHDC2
• Synonyms: DKFZp686F0985; DKFZp686K13244; FLJ10948; FLJ45240; FLJ51075; FLJ52213; FLJ52450; FLJ78805
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231091 representing NM_001198962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCGTTCTGTGCCTCCTGCGCCCCTGGAGGCCCCTTCGGGCCCGCGGCTGCGCTTCCGACGGGG
  CGGCCGGGGGCTCAGAGATCCAAGTGCGCGCCCTGGCGGGTCCGGACCAAGGGATCACTGAGATTCTGAT
  GAACAGACCTTCTGCCCGCAATGCCTTGGGGAATGTCTTCGTCAGTGAGCTGCTGGAAACTCTGGCCCAG
  CTGCGGGAGGACCGGCAAGTGCGTGTCCTGCTCTTCAGAAGTGGAGTGAAGGGCGTGTTCTGTGCAGGTG
  CAGACCTGAAGGAGCGGGAACAGATGAGTGAAGCAGAGGTGGGGGTGTTTGTCCAGCGACTCCGGGGCCT
  GATGAATGACATCGCTTCCTCGGCAGTCATGGGACTGATTGAGACCACGCGAGGGCTCCTCCCGGGGGCA
  GGAGGGACTCAGAGGCTGCCCCGTTGTCTGGGGGTGGCCCTGGCGAAGGAGCTCATCTTCACGGGCCGAC
  GACTGAGTGGAACTGAGGCCCACGTACTGGGGCTGGTGAATCACGCTGTGGCCCAGAACGAGGAGGGGGA
  CGCCGCCTACCAGCGGGCACGAGCACTGGCCCAGGAGATCCTGCCCCAGGTGGACATTGCATCTGGGATG
  GCCATTGAAGGGATGTGCTATGCCCAGAATATTCCAACCCGGGACCGGCTAGAGGGCATGGCAGCCTTCA
  GGGAGAAGCGGACTCCCAAATTTGTTGGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231091 representing NM_001198962
  Red=Cloning site Green=Tags(s)
  
  MLRVLCLLRPWRPLRARGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSELLETLAQ
  LREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDIASSAVMGLIETTRGLLPGA
  GGTQRLPRCLGVALAKELIFTGRRLSGTEAHVLGLVNHAVAQNEEGDAAYQRARALAQEILPQVDIASGM
  AIEGMCYAQNIPTRDRLEGMAAFREKRTPKFVGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198962
• ORF Size: 732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198962.1, NP_001185891.1
• RefSeq ORF: 735
• Locus ID: 55268
• MW: 26.8 kDa