STEAP4 (NM_001205315) Human Tagged ORF Clone

CAT#: RC232783

STEAP4 (Myc-DDK tagged) - Homo sapiens STEAP family member 4 (STEAP4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STEAP4
• Synonyms: SchLAH; STAMP2; TIARP; TNFAIP9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232783 representing NM_001205315
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAACTTGTATAGATGCACTTCCTCTTACTATGAATTCTTCAGAAAAGCAAGAGACTGTATGTA
  TTTTTGGAACTGGTGATTTTGGAAGATCACTGGGATTGAAAATGCTCCAGTGTGGTTATTCTGTTGTTTT
  TGGAAGTCGAAACCCCCAGAAGACCACCCTACTGCCCAGTGGTGCAGAAGTCTTGAGCTATTCAGAAGCA
  GCCAAGAAGTCTGACATCATAATCATAGCAATCCACAGAGAGCATTATGATTTTCTCACAGAATTAACTG
  AGGTTCTCAATGGAAAAATATTGGTAGACATCAGCAACAACCTCAAAATCAATCAATATCCAGAATCTAA
  TGCAGAGTACCTTGCTCATTTGGTGCCAGGAGCCCACGTGGTAAAAGCATTTAACACCATCTCAGCCTGG
  GCTCTCCAGTCAGGAGCACTGGATGCAAGTCGGCAGGTGTTTGTGTGTGGAAATGACAGCAAAGCCAAGC
  AAAGAGTGATGGATATTGTTCGTAATCTTGGACTTACTCCAATGGATCAAGGATCACTCATGGCAGCCAA
  AGAAATTGAAAAGTACCCCCTGCAGCTATTTCCAATGTGGAGGTTCCCCTTCTATTTGTCTGCTGTGCTG
  TGTGTCTTCTTGTTTTTCTATTGTGTTATAAGAGACGTAATCTACCCTTATGTTTATGAAAAGAAAGATA
  ATACATTTCGTATGGCTATTTCCATTCCAAATCGTATCTTTCCAATAACAGCACTTACACTGCTTGCTTT
  GGTTTACCTCCCTGGTGTTATTGCTGCCATTCTACAACTGTACCGAGGCACAAAATACCGTCGATTCCCA
  GACTGGCTTGACCACTGGATGCTTTGCCGAAAGCAGCTTGGCTTGGTAGCTCTGGGATTTGCCTTCCTTC
  ATGTCCTCTACACACTTGTGATTCCTATTCGATATTATGTACGATGGAGATTGGGAAACTTAACCGTTAC
  CCAGGCAATACTCAAGAAGGAGAATCCATTTAGCACCTCCTCAGCCTGGCTCAGTGATTCATATGTGGCT
  TTGGGAATACTTGGGTTTTTTCTGTTTGTACTCTTGGGAATCACTTCTTTGCCATCTGTTAGCAATGCAG
  TCAACTGGAGAGAGTTCCGATTTGTCCAGTCCAAACTGGGTTATTTGACCCTGATCTTGTGTACAGCCCA
  CACCCTGGTGTACGGTGGGAAGAGATTCCTCAGCCCTTCAAATCTCAGATGGTATCTTCCTGCAGCCTAC
  GTGTTAGGGCTTATCATTCCTTGCACTGTGCTGGTGATCAAGTTTGTCCTAATCATGCCATGTGTAGACA
  ACACCCTTACAAGGATCCGCCAGGGCTGGGAAAGGAACTCAAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232783 representing NM_001205315
  Red=Cloning site Green=Tags(s)
  
  MEKTCIDALPLTMNSSEKQETVCIFGTGDFGRSLGLKMLQCGYSVVFGSRNPQKTTLLPSGAEVLSYSEA
  AKKSDIIIIAIHREHYDFLTELTEVLNGKILVDISNNLKINQYPESNAEYLAHLVPGAHVVKAFNTISAW
  ALQSGALDASRQVFVCGNDSKAKQRVMDIVRNLGLTPMDQGSLMAAKEIEKYPLQLFPMWRFPFYLSAVL
  CVFLFFYCVIRDVIYPYVYEKKDNTFRMAISIPNRIFPITALTLLALVYLPGVIAAILQLYRGTKYRRFP
  DWLDHWMLCRKQLGLVALGFAFLHVLYTLVIPIRYYVRWRLGNLTVTQAILKKENPFSTSSAWLSDSYVA
  LGILGFFLFVLLGITSLPSVSNAVNWREFRFVQSKLGYLTLILCTAHTLVYGGKRFLSPSNLRWYLPAAY
  VLGLIIPCTVLVIKFVLIMPCVDNTLTRIRQGWERNSKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205315
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205315.1, NP_001192244.1
• RefSeq Size: 4587 bp
• RefSeq ORF: 1380 bp
• Locus ID: 79689
• Protein Families: Druggable Genome, Transmembrane
• MW: 52 kDa
• Gene Summary: The protein encoded by this gene belongs to the STEAP (six transmembrane epithelial antigen of prostate) family, and resides in the golgi apparatus. It functions as a metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+), using NAD(+) as acceptor. Studies in mice and human suggest that this gene maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]