CRMP2 (DPYSL2) (NM_001244604) Human Tagged ORF Clone

CAT#: RC232947

DPYSL2 (Myc-DDK tagged) - Homo sapiens dihydropyrimidinase-like 2 (DPYSL2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPYSL2
• Synonyms: CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232947 representing NM_001244604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATGGGTTGATCAAGCAAATAGGAGAAAATCTGATTGTGCCAGGAGGAGTGAAGACCATCGAGG
  CCCACTCCCGGATGGTGATCCCCGGAGGAATTGACGTCCACACTCGTTTCCAGATGCCTGATCAGGGAAT
  GACGTCTGCTGATGATTTCTTCCAAGGAACCAAGGCGGCCCTGGCTGGGGGAACCACTATGATCATTGAC
  CACGTTGTTCCTGAGCCTGGGACAAGCCTGCTCGCTGCCTTTGACCAGTGGAGGGAATGGGCCGACAGCA
  AGTCCTGCTGTGACTACTCTCTGCATGTGGACATCAGTGAGTGGCATAAGGGCATCCAGGAGGAGATGGA
  AGCGCTTGTGAAGGATCACGGGGTAAATTCCTTCCTCGTGTACATGGCTTTCAAAGATCGCTTCCAGCTA
  ACGGATTGCCAGATTTATGAAGTACTGAGTGTGATCCGGGATATTGGCGCCATAGCCCAAGTCCACGCAG
  AAAATGGCGACATCATTGCAGAGGAGCAGCAGAGGATCCTGGATCTGGGCATCACGGGCCCCGAGGGACA
  TGTGCTGAGCCGACCTGAGGAGGTCGAGGCCGAAGCCGTGAATCGTGCCATCACCATCGCCAACCAGACC
  AACTGCCCGCTGTATATCACCAAGGTGATGAGCAAAAGCTCTGCTGAGGTCATCGCCCAGGCACGGAAGA
  AGGGAACTGTGGTGTATGGCGAGCCCATCACTGCCAGCTTGGGAACGGACGGCTCCCATTACTGGAGCAA
  GAACTGGGCCAAGGCTGCTGCCTTTGTCACCTCCCCACCCTTGAGCCCTGATCCAACCACTCCAGACTTT
  CTCAACTCCTTGCTGTCCTGTGGAGACCTCCAGGTCACGGGCAGTGCCCATTGCACGTTTAACACTGCCC
  AGAAGGCTGTAGGAAAGGACAACTTCACCCTGATTCCGGAGGGCACCAATGGCACTGAGGAGCGGATGTC
  CGTCATCTGGGACAAGGCTGTGGTCACTGGGAAGATGGATGAGAACCAGTTTGTGGCTGTGACCAGCACC
  AATGCAGCCAAAGTCTTCAACCTTTACCCCCGGAAAGGCCGCATTGCTGTGGGATCCGATGCCGACCTGG
  TCATCTGGGACCCCGACAGCGTTAAAACCATCTCTGCCAAGACACACAACAGCTCTCTCGAGTACAACAT
  CTTTGAAGGCATGGAGTGCCGCGGCTCCCCACTGGTGGTCATCAGCCAGGGGAAGATTGTCCTGGAGGAC
  GGCACCCTGCATGTCACCGAAGGCTCTGGACGCTACATTCCCCGGAAGCCCTTCCCTGATTTTGTTTACA
  AGCGTATCAAGGCAAGGAGCAGGCTGGCTGAGCTGAGAGGGGTTCCTCGTGGCCTGTATGACGGACCTGT
  GTGTGAAGTGTCTGTGACGCCCAAGACAGTCACTCCAGCCTCCTCGGCCAAGACGTCTCCTGCCAAGCAG
  CAGGCCCCACCTGTCCGGAACCTGCACCAGTCTGGATTCAGTTTGTCTGGTGCTCAGATTGATGACAACA
  TTCCCCGCCGCACCACCCAGCGTATCGTGGCGCCCCCCGGTGGCCGTGCCAACATCACCAGCCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232947 representing NM_001244604
  Red=Cloning site Green=Tags(s)
  
  MEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIID
  HVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQL
  TDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQT
  NCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDF
  LNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTST
  NAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLED
  GTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQ
  QAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244604
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244604.1, NP_001231533.1
• RefSeq Size: 4297 bp
• RefSeq ORF: 1611 bp
• Locus ID: 1808
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 58.6 kDa
• Gene Summary: 'This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'