RGS3 (NM_001276261) Human Tagged ORF Clone

CAT#: RC232998

RGS3 (Myc-DDK tagged) - Homo sapiens regulator of G-protein signaling 3 (RGS3), transcript variant 8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGS3
• Synonyms: C2PA; RGP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232998 representing NM_001276261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGCTTCAATGGGCTCTGCAAGGTGTGCTCGGAGCGCCGCTACCGCCAGATCACCATCCCGAGGG
  GAAAGGACGGCTTTGGCTTCACCATCTGCTGCGACTCTCCAGTTCGAGTCCAGGCCGTGGATTCCGGGGG
  TCCGGCGGAACGGGCAGGGCTGCAGCAGCTGGACACGGTGCTGCAGCTGAATGAGAGGCCTGTGGAGCAC
  TGGAAATGTGTGGAGCTGGCCCACGAGATCCGGAGCTGCCCCAGTGAGATCATCCTACTCGTGTGGCGCA
  TGGTCCCCCAGGTCAAGCCAGGACCAGATGGCGGGGTCCTGCGGCGGGCCTCCTGCAAGTCGACACATGA
  CCTCCAGTCACCCCCCAACAAACGGGAGAAGAACTGCACCCATGGGGTCCAGGCACGGCCTGAGCAGCGC
  CACAGCTGCCACCTGGTATGTGACAGCTCTGATGGGCTGCTGCTCGGCGGCTGGGAGCGCTACACCGAGG
  TGGCCAAGCGCGGGGGCCAGCACACCCTGCCTGCACTGTCCCGTGCCACTGCCCCCACCGACCCCAACTA
  CATCATCCTGGCCCCGCTGAATCCTGGGAGCCAGCTGCTCCGGCCTGTGTACCAGGAGGATACCATCCCC
  GAAGAATCAGGGAGTCCCAGTAAAGGGAAGTCCTACACAGGCCTGGGGAAGAAGTCCCGGCTGATGAAGA
  CAGTGCAGACCATGAAGGGCCACGGGAACTACCAAAACTGCCCGGTTGTGAGGCCGCATGCCACGCACTC
  AAGCTATGGCACCTACGTCACCCTGGCCCCCAAAGTCCTGGTGTTCCCTGTCTTTGTTCAGCCTCTAGAT
  CTCTGTAATCCTGCCCGGACCCTCCTGCTGTCAGAGGAGCTGCTGCTGTATGAAGGGAGGAACAAGGCTG
  CCGAGGTGACACTGTTTGCCTATTCGGACCTGCTGCTCTTCACCAAGGAGGACGAGCCTGGCCGCTGCGA
  CGTCCTGAGGAACCCCCTCTACCTCCAGAGTGTGAAGCTGCAGGAAGGTTCTTCAGAAGACCTGAAATTC
  TGCGTGCTCTATCTAGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAAGCGCACTCGCAGGAGCAGAAGA
  AGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAGCTGGCAGCATCACCCCCGGACAG
  CAAGATGAGCGGGGCTGACACCGTTGGGGATGATGACGAAGCCTCCCGGAAGAGAAAGAGCAAAAACCTA
  GCCAAGGACATGAAGAACAAGCTGGGGATCTTCAGACGGCGGAATGAGTCCCCTGGAGCCCCTCCCGCGG
  GCAAGGCAGACAAAATGATGAAGTCATTCAAGCCCACCTCAGAGGAAGCCCTCAAGTGGGGCGAGTCCTT
  GGAGAAGCTGCTGGTTCACAAATACGGGTTAGCAGTGTTCCAAGCCTTCCTTCGCACTGAGTTCAGTGAG
  GAGAATCTGGAGTTCTGGTTGGCTTGTGAGGACTTCAAGAAGGTCAAGTCACAGTCCAAGATGGCATCCA
  AGGCCAAGAAGATCTTTGCTGAATACATCGCGATCCAGGCATGCAAGGAGGTCAACCTGGACTCCTACAC
  GCGGGAGCACACCAAGGACAACCTGCAGAGCGTCACGCGGGGCTGCTTCGACCTGGCACAGAAGCGCATC
  TTCGGGCTCATGGAAAAGGACTCGTACCCTCGCTTTCTCCGTTCTGACCTCTACCTGGACCTTATTAACC
  AGAAGAAGATGAGTCCCCCGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232998 representing NM_001276261
  Red=Cloning site Green=Tags(s)
  
  MNRFNGLCKVCSERRYRQITIPRGKDGFGFTICCDSPVRVQAVDSGGPAERAGLQQLDTVLQLNERPVEH
  WKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRRASCKSTHDLQSPPNKREKNCTHGVQARPEQR
  HSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRATAPTDPNYIILAPLNPGSQLLRPVYQEDTIP
  EESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPVVRPHATHSSYGTYVTLAPKVLVFPVFVQPLD
  LCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTKEDEPGRCDVLRNPLYLQSVKLQEGSSEDLKF
  CVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAASPPDSKMSGADTVGDDDEASRKRKSKNL
  AKDMKNKLGIFRRRNESPGAPPAGKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAFLRTEFSE
  ENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRI
  FGLMEKDSYPRFLRSDLYLDLINQKKMSPPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276261
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276261.1, NP_001263190.1
• RefSeq Size: 2735 bp
• RefSeq ORF: 1776 bp
• Locus ID: 5998
• Cytogenetics: 9q32
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 66.9 kDa
• Gene Summary: 'This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]'