DGKH (NM_001204506) Human Tagged ORF Clone

CAT#: RC233198

DGKH (Myc-DDK tagged) - Homo sapiens diacylglycerol kinase, eta (DGKH), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGKH
• Synonyms: DGKeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233198 representing NM_001204506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGTGCTGAGAACAGAAAGGAGATGGAGGATTGGATCAGCTCACTGAAGTCTGTACAGACCAGAG
  AACCCTACGAGGTGGCCCAGTTTAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCA
  CGCCCGACCCACCTTCTGTAACGTGTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGC
  GAAGTGTGTAAATTCAAGGCTCACAAAAGATGTGCAGTGAGAGCAACAAATAACTGTAAATGGACTACCC
  TGGCCTCCATCGGGAAGGACATTATAGAAGATGAAGATGGCGTCGCGATGCCTCACCAGTGGCTTGAGGG
  CAACCTGCCTGTAAGTGCCAAGTGTGCTGTCTGCGACAAAACATGTGGCAGTGTTCTCCGTCTACAGGAT
  TGGAAATGCCTTTGGTGTAAGACAATGGTACACACTGCCTGCAAAGATTTATACCATCCAATATGTCCAC
  TTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGCACCGATTCCGATGGTTTCTGTAG
  AGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATTCTAAGAGTGGAGATAATCAGGGA
  GTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGTGTTTGATTTAATGAATGGAGGTC
  CTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATTCTTGTTTGTGGAGGCGATGGAAG
  TGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAACAGTGTCAGCTGGGAGTGTTGCCT
  TTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTCATATGACGATGACACCCAACTTC
  CTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGACAGGTGGAGTATAATGACATATGA
  ACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAGCATCTGAAGAATTTTATATGACG
  ATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTCTGATGAACATGCAGTGGTCATAT
  CTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAAGTAGAAAAGACGTATGACAAAAC
  CTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAGTCCTAAACGAGAAGCTCGAACAA
  CTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCCTGGCCTCAGCCCTCTCATTGTGG
  AAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGCAAAGAGCAGCTTGGGGATGACGT
  TACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGTTGCGGGCAAATAGTTTAAAGAAA
  GCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCCGACAGTTCACCCCTGTGAACCAG
  CTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAGGAGGAAGCTAAAGATGATGGTGC
  CAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCCCGGGCAAGTTATGGCCATTCCCAA
  ACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCTCCCTGTGCTCAATACCAGAATAA
  TCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGTTCGATTATCAACAAAATGTTACT
  GGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCGGATCTAGATTCCGTAGATGGATATTCA
  GAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGATGCAAAAATTTCATTAGAATTTAATAATA
  AAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTGATGTGGTATGGAGTCCTTGGAAC
  CCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAAAGGGTTCAACTTGAGTGTGATGGGCAGTAT
  ATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAGCTATGCTGGAGGCACTAACTTTT
  GGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCATCCTTTGATGACAAGATCCTGGAAGTTGTAGC
  AATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTCATTAAACTGCAGCATCATCGAATAGCCCAGTGC
  CGTACAGTGAAAATCACTATATTTGGTGACGAAGGAGTCCCAGTGCAAGTGGATGGTGAAGCGTGGGTTC
  AGCCTCCAGGGATTATCAAAATTGTGCACAAAAACAGAGCACAAATGCTAACAAGGGACAGAGCCTTTGA
  GAGCACTCTGAAATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTAAACCAGTTCTCCGAACCCATTTG
  TACATCCATCACGCCATTGACTTGGCAACAGAAGAGGTGTCGCAGATGCAGCTATGCTCCCAGGCTGCAG
  AGGAGCTCATTACTAGGATATGTGACGCAGCCACAATTCACTGTCTTTTGGAGCAAGAACTGGCCCATGC
  TGTGAATGCCTGCTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCCCGGAGAGTCTTACAAGAGACACT
  GCCACTGAAATAGCCATCAATGTGAAGGCGCTGTATAATGAAACAGAATCTTTGCTAGTTGGCAGGGTTC
  CTTTGCAGCTGGAATCGCCACATGAAGAGCGAGTATCCAATGCCTTACACTCTGTGGAGGTGGAATTACA
  GAAACTGACAGAGATTCCTTGGCTTTATTATATCTTACACCCAAATGAGGATGAGGAACCTCCTATGGAT
  TGCACCAAAAGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAAGTTTAAAAAGGAAAAGGTTCAGA
  AGCAGAAGACAAGTTCACAGCCTGTTCAGAAATGGGGCACAGAGGAAGTTGCTGCTTGGCTGGATCTGCT
  CAATTTGGGAGAGTACAAAGATATCTTCATCCGTCATGACATCAGAGGGGCTGAACTTTTGCATCTGGAA
  AGGCGAGATCTTAAGGATCTGGGGATACCGAAAGTGGGTCATGTGAAGCGAATTCTCCAGGGAATTAAAG
  AGCTTGGAAGGAGCACTCCACAGTCGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233198 representing NM_001204506
  Red=Cloning site Green=Tags(s)
  
  MLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSC
  EVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQD
  WKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQG
  VKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLP
  LGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMT
  IYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQ
  LLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKK
  AVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQ
  TDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYS
  EKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQY
  IPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQC
  RTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHL
  YIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDT
  ATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMD
  CTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLE
  RRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204506
• ORF Size: 3252 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204506.1, NM_001204506.2, NP_001191435.1
• RefSeq Size: 3956
• RefSeq ORF: 3255
• Locus ID: 160851
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 121 kDa
• Gene Summary: This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]