EAAT2 (SLC1A2) (NM_001252652) Human Tagged ORF Clone

CAT#: RC234446

SLC1A2 (Myc-DDK tagged) - Homo sapiens solute carrier family 1 (glial high affinity glutamate transporter), member 2 (SLC1A2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC1A2
• Synonyms: EAAT2; EIEE41; GLT-1; HBGT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234446 representing NM_001252652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTACGGAAGGTGCCAACAATATGCCCAAGCAGGTGGAAGTGCGAATGCACGACAGTCATCTTG
  GCTCAGAGGAACCCAAGCACCGGCACCTGGGCCTGCGCCTGTGTGACAAGCTGGGGAAGAATCTGCTGCT
  CACCCTGACGGTGTTTGGTGTCATCCTGGGAGCAGTGTGTGGAGGGCTTCTTCGCTTGGCATCTCCCATC
  CACCCTGATGTGGTTATGTTAATAGCCTTCCCAGGGGATATACTCATGAGGATGCTAAAAATGCTCATTC
  TCCCTCTAATCATCTCCAGCTTAATCACAGGGTTGTCAGGCCTGGATGCTAAGGCTAGTGGCCGCTTGGG
  CACGAGAGCCATGGTGTATTACATGTCCACGACCATCATTGCTGCAGTACTGGGGGTCATTCTGGTCTTG
  GCTATCCATCCAGGCAATCCCAAGCTCAAGAAGCAGCTGGGGCCTGGGAAGAAGAATGATGAAGTGTCCA
  GCCTGGATGCCTTCCTGGACCTTATTCGAAATCTCTTCCCTGAAAACCTTGTCCAAGCCTGCTTTCAACA
  GATTCAAACAGTGACGAAGAAAGTCCTGGTTGCACCACCGCCGGACGAGGAGGCCAACGCAACCAGCGCT
  GTTGTCTCTCTGTTGAACGAGACTGTGACTGAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAAGGGCC
  TGGAGTTCAAGGATGGGATGAACGTCTTAGGTCTGATAGGGTTTTTCATTGCTTTTGGCATCGCTATGGG
  GAAGATGGGAGATCAGGCCAAGCTGATGGTGGATTTCTTCAACATTTTGAATGAGATTGTAATGAAGTTA
  GTGATCATGATCATGTGGTACTCTCCCCTGGGTATCGCCTGCCTGATCTGTGGAAAGATCATTGCAATCA
  AGGACTTAGAAGTGGTTGCTAGGCAACTGGGGATGTACATGGTAACAGTGATCATAGGCCTCATCATCCA
  CGGGGGCATCTTTCTCCCCTTGATTTACTTTGTAGTGACCAGGAAAAACCCCTTCTCCTTTTTTGCTGGC
  ATTTTCCAAGCTTGGATCACTGCCCTGGGCACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTTTCGTT
  GCCTGGAAGAAAATCTGGGGATTGATAAGCGTGTGACTAGATTCGTCCTTCCTGTTGGAGCAACCATTAA
  CATGGATGGTACAGCCCTTTATGAAGCGGTAGCCGCCATCTTTATAGCCCAAATGAATGGTGTTGTCCTG
  GATGGAGGACAGATTGTGACTGTAAGCCTCACAGCCACCCTGGCAAGCGTCGGCGCGGCCAGTATCCCCA
  GTGCCGGGCTGGTCACCATGCTCCTCATTCTGACAGCCGTGGGCCTGCCAACAGAGGACATCAGCCTGCT
  GGTGGCTGTGGACTGGCTGCTGGACAGGATGAGAACTTCAGTCAATGTTGTGGGTGACTCTTTTGGGGCT
  GGGATAGTCTATCACCTCTCCAAGTCTGAGCTGGATACCATTGACTCCCAGCATCGAGTGCATGAAGATA
  TTGAAATGACCAAGACTCAATCCATTTATGATGACATGAAGAACCACAGGGAAAGCAACTCTAATCAATG
  TGTCTATGCTGCACACAACTCTGTCATAGTAGATGAATGCAAGGTAACTCTGGCAGCCAATGGAAAGTCA
  GCCGACTGCAGTGTTGAGGAAGAACCTTGGAAACGTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234446 representing NM_001252652
  Red=Cloning site Green=Tags(s)
  
  MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPI
  HPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVL
  AIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSA
  VVSLLNETVTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKL
  VIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAG
  IFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVL
  DGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGA
  GIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKS
  ADCSVEEEPWKREK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252652
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252652.1, NP_001239581.1
• RefSeq Size: 11724 bp
• RefSeq ORF: 1698 bp
• Locus ID: 6506
• Cytogenetics: 11p13
• Protein Families: Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• MW: 62.1 kDa
• Gene Summary: 'This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]'