EAAT2 (SLC1A2) (NM_001252652) Human Tagged ORF Clone

CAT#: RG234446

SLC1A2 (GFP-tagged) - Homo sapiens solute carrier family 1 (glial high affinity glutamate transporter), member 2 (SLC1A2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC1A2
• Synonyms: EAAT2; EIEE41; GLT-1; HBGT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234446 representing NM_001252652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAGCAGGTGGAAGTGCGAATGCACGACAGTCATCTTGGCTCAGAGGAACCCAAGCACCGGCACC
  TGGGCCTGCGCCTGTGTGACAAGCTGGGGAAGAATCTGCTGCTCACCCTGACGGTGTTTGGTGTCATCCT
  GGGAGCAGTGTGTGGAGGGCTTCTTCGCTTGGCATCTCCCATCCACCCTGATGTGGTTATGTTAATAGCC
  TTCCCAGGGGATATACTCATGAGGATGCTAAAAATGCTCATTCTCCCTCTAATCATCTCCAGCTTAATCA
  CAGGGTTGTCAGGCCTGGATGCTAAGGCTAGTGGCCGCTTGGGCACGAGAGCCATGGTGTATTACATGTC
  CACGACCATCATTGCTGCAGTACTGGGGGTCATTCTGGTCTTGGCTATCCATCCAGGCAATCCCAAGCTC
  AAGAAGCAGCTGGGGCCTGGGAAGAAGAATGATGAAGTGTCCAGCCTGGATGCCTTCCTGGACCTTATTC
  GAAATCTCTTCCCTGAAAACCTTGTCCAAGCCTGCTTTCAACAGATTCAAACAGTGACGAAGAAAGTCCT
  GGTTGCACCACCGCCGGACGAGGAGGCCAACGCAACCAGCGCTGTTGTCTCTCTGTTGAACGAGACTGTG
  ACTGAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAAGGGCCTGGAGTTCAAGGATGGGATGAACGTCT
  TAGGTCTGATAGGGTTTTTCATTGCTTTTGGCATCGCTATGGGGAAGATGGGAGATCAGGCCAAGCTGAT
  GGTGGATTTCTTCAACATTTTGAATGAGATTGTAATGAAGTTAGTGATCATGATCATGTGGTACTCTCCC
  CTGGGTATCGCCTGCCTGATCTGTGGAAAGATCATTGCAATCAAGGACTTAGAAGTGGTTGCTAGGCAAC
  TGGGGATGTACATGGTAACAGTGATCATAGGCCTCATCATCCACGGGGGCATCTTTCTCCCCTTGATTTA
  CTTTGTAGTGACCAGGAAAAACCCCTTCTCCTTTTTTGCTGGCATTTTCCAAGCTTGGATCACTGCCCTG
  GGCACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTTTCGTTGCCTGGAAGAAAATCTGGGGATTGATA
  AGCGTGTGACTAGATTCGTCCTTCCTGTTGGAGCAACCATTAACATGGATGGTACAGCCCTTTATGAAGC
  GGTAGCCGCCATCTTTATAGCCCAAATGAATGGTGTTGTCCTGGATGGAGGACAGATTGTGACTGTAAGC
  CTCACAGCCACCCTGGCAAGCGTCGGCGCGGCCAGTATCCCCAGTGCCGGGCTGGTCACCATGCTCCTCA
  TTCTGACAGCCGTGGGCCTGCCAACAGAGGACATCAGCCTGCTGGTGGCTGTGGACTGGCTGCTGGACAG
  GATGAGAACTTCAGTCAATGTTGTGGGTGACTCTTTTGGGGCTGGGATAGTCTATCACCTCTCCAAGTCT
  GAGCTGGATACCATTGACTCCCAGCATCGAGTGCATGAAGATATTGAAATGACCAAGACTCAATCCATTT
  ATGATGACATGAAGAACCACAGGGAAAGCAACTCTAATCAATGTGTCTATGCTGCACACAACTCTGTCAT
  AGTAGATGAATGCAAGGTAACTCTGGCAGCCAATGGAAAGTCAGCCGACTGCAGTGTTGAGGAAGAACCT
  TGGAAACGTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234446 representing NM_001252652
  Red=Cloning site Green=Tags(s)
  
  MPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIA
  FPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKL
  KKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETV
  TEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSP
  LGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITAL
  GTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVS
  LTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKS
  ELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEP
  WKREK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252652
• ORF Size: 1695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252652.1, NP_001239581.1
• RefSeq Size: 11724 bp
• RefSeq ORF: 1698 bp
• Locus ID: 6506
• Cytogenetics: 11p13
• Protein Families: Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: 'This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]'