EAAT2 (SLC1A2) (NM_001252652) Human Untagged Clone

CAT#: SC332208

SLC1A2 (untagged) - Homo sapiens solute carrier family 1 (glial high affinity glutamate transporter), member 2 (SLC1A2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC1A2
• Synonyms: EAAT2; EIEE41; GLT-1; HBGT
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001252652, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCAAGCAGGTGGAAGTGCGAATGCACGACAGTCATCTTGGCTCAGAGGAACCCAAGCACCGGCACC
  TGGGCCTGCGCCTGTGTGACAAGCTGGGGAAGAATCTGCTGCTCACCCTGACGGTGTTTGGTGTCATCCT
  GGGAGCAGTGTGTGGAGGGCTTCTTCGCTTGGCATCTCCCATCCACCCTGATGTGGTTATGTTAATAGCC
  TTCCCAGGGGATATACTCATGAGGATGCTAAAAATGCTCATTCTCCCTCTAATCATCTCCAGCTTAATCA
  CAGGGTTGTCAGGCCTGGATGCTAAGGCTAGTGGCCGCTTGGGCACGAGAGCCATGGTGTATTACATGTC
  CACGACCATCATTGCTGCAGTACTGGGGGTCATTCTGGTCTTGGCTATCCATCCAGGCAATCCCAAGCTC
  AAGAAGCAGCTGGGGCCTGGGAAGAAGAATGATGAAGTGTCCAGCCTGGATGCCTTCCTGGACCTTATTC
  GAAATCTCTTCCCTGAAAACCTTGTCCAAGCCTGCTTTCAACAGATTCAAACAGTGACGAAGAAAGTCCT
  GGTTGCACCACCGCCGGACGAGGAGGCCAACGCAACCAGCGCTGTTGTCTCTCTGTTGAACGAGACTGTG
  ACTGAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAAGGGCCTGGAGTTCAAGGATGGGATGAACGTCT
  TAGGTCTGATAGGGTTTTTCATTGCTTTTGGCATCGCTATGGGGAAGATGGGAGATCAGGCCAAGCTGAT
  GGTGGATTTCTTCAACATTTTGAATGAGATTGTAATGAAGTTAGTGATCATGATCATGTGGTACTCTCCC
  CTGGGTATCGCCTGCCTGATCTGTGGAAAGATCATTGCAATCAAGGACTTAGAAGTGGTTGCTAGGCAAC
  TGGGGATGTACATGGTAACAGTGATCATAGGCCTCATCATCCACGGGGGCATCTTTCTCCCCTTGATTTA
  CTTTGTAGTGACCAGGAAAAACCCCTTCTCCTTTTTTGCTGGCATTTTCCAAGCTTGGATCACTGCCCTG
  GGCACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTTTCGTTGCCTGGAAGAAAATCTGGGGATTGATA
  AGCGTGTGACTAGATTCGTCCTTCCTGTTGGAGCAACCATTAACATGGATGGTACAGCCCTTTATGAAGC
  GGTAGCCGCCATCTTTATAGCCCAAATGAATGGTGTTGTCCTGGATGGAGGACAGATTGTGACTGTAAGC
  CTCACAGCCACCCTGGCAAGCGTCGGCGCGGCCAGTATCCCCAGTGCCGGGCTGGTCACCATGCTCCTCA
  TTCTGACAGCCGTGGGCCTGCCAACAGAGGACATCAGCCTGCTGGTGGCTGTGGACTGGCTGCTGGACAG
  GATGAGAACTTCAGTCAATGTTGTGGGTGACTCTTTTGGGGCTGGGATAGTCTATCACCTCTCCAAGTCT
  GAGCTGGATACCATTGACTCCCAGCATCGAGTGCATGAAGATATTGAAATGACCAAGACTCAATCCATTT
  ATGATGACATGAAGAACCACAGGGAAAGCAACTCTAATCAATGTGTCTATGCTGCACACAACTCTGTCAT
  AGTAGATGAATGCAAGGTAACTCTGGCAGCCAATGGAAAGTCAGCCGACTGCAGTGTTGAGGAAGAACCT
  TGGAAACGTGAGAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252652
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252652.1, NP_001239581.1
• RefSeq Size: 11724 bp
• RefSeq ORF: 1698 bp
• Locus ID: 6506
• Cytogenetics: 11p13
• Protein Families: Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: 'This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]'
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which is shorter than isoform 1.