UTY (NM_001258251) Human Tagged ORF Clone

CAT#: RC235280

UTY (Myc-DDK tagged) - Homo sapiens ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (UTY), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UTY
• Synonyms: KDM6AL; KDM6C; UTY1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235280 representing NM_001258251
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG
  AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG
  TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA
  GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT
  GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA
  CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
  AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA
  AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT
  TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT
  TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC
  TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG
  AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
  GGCCAATCGTGGTATTTTCTTGGAAGGTGTTATTCAAGTATTGGGAAAGTTCAGGATGCCTTTATATCTT
  ACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGTTGTATCAGCA
  GCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACAATTGGACCATGGGCATGCCGCA
  GCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAAGATGCCATTAAATGCTACCTAA
  ATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAAGAATTAAATTTCTACAGAATGG
  TTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACAGCAAGTTTATTCGTTGTGTTTG
  ACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGATAATTTAAATCCAGCACAGAAGC
  ATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGAGACACAAAGAAGTTGCTCAGGT
  ACGAACTACTGGAATTCATAACGGGGCCATAACTGATTCATCACTGCCTACAAACTCTGTCTCTAATCGA
  CAACCACATGGTGCTCTGACCAGAGTATCTAGCGTCTCTCAGCCTGGAGTTCGCCCTGCTTGTGTTGAAA
  AACTTTTGTCCAGTGGAGCTTTTTCTGCAGGCTGTATTCCTTGTGGCACATCAAAAATTCTAGGAAGTAC
  AGACACTATCTTGCTAGGCAGTAATTGTATAGCAGGAAGTGAAAGTAATGGAAATGTGCCTTACCTGCAG
  CAAAATACACACACTCTACCTCATAATCATACAGACCTGAACAGCAGCACAGAAGAGCCATGGAGAAAAC
  AGCTATCTAACTCCGCTCAGGCCTACCTTGGACATCTTACAGGTTTCATACAAGACAACTGCAATAAAGG
  GCTTCATAAAAGTCAGAGTTCATGTTTGTCAGGACCTAATGAAGAACAACCTCTGTTTTCCACTGGGTCA
  GCCCAGTATCACCAGGCAACTAGCACTGGTATTAAGAAGGCGAATGAACATCTCACTCTGCCTAGTAATT
  CAGTACCACAGGGGGATGCTGACAGTCACCTCTCCTGTCATACTGCTACCTCAGGTGGACAACAAGGCAT
  TATGTTTACCAAAGAGAGCAAGCCTTCAAAAAATAGATCCTTGGTGCCTGAAACAAGCAGGCATACTGGA
  GACACATCTAATGGCTGTGCTGATGTCAAGGGACTTTCTAATCATGTTCATCAGTTGATAGCAGATGCTG
  TTTCCAGTCCTAACCATGGAGATTCACCAAATTTATTAATTGCAGACAATCCTCAGCTCTCTGCTTTGTT
  GATTGGAAAAGCCAATGGCAATGTGGGTACTGGAACCTGTGACAAAGTGAATAATATTCACCCAGCTGTT
  CATACAAAGACTGATCATTCTGTTGCCTCTTCACCCTCTTCAGCCATTTCCACAGCAACACCTTCTCCTA
  AATCCACTGAGCAGAGAAGCATAAACAGTGTTACCAGCCTTAACAGTCCTCACAGTGGATTACACACAGT
  CAATGGAGAGGGGCTGGGGAAGTCACAGAGCTCTACAAAAGTAGACCTGCCTTTAGCTAGCCACAGATCT
  ACTTCTCAGATCTTACCATCAATGTCAGTGTCTATATGCCCCAGTTCAACAGAAGTTCTGAAAGCATGCA
  GGAATCCAGGTAAAAATGGCTTGTCTAATAGCTGCATTTTGTTAGATAAATGTCCACCTCCAAGACCACC
  AACTTCACCATACCCACCCTTGCCAAAGGACAAGTTGAATCCACCCACACCTAGTATTTACTTGGAAAAT
  AAACGTGATGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCAAAAAACCCTGTTACAGTAATAC
  GTGGCCTTGCTGGAGCTCTTAAATTAGATCTTGGACTTTTCTCTACCAAAACTTTGGTAGAAGCTAACAA
  TGAACATATGGTAGAAGTGAGGACACAGTTGCTGCAACCAGCAGATGAAAACTGGGATCCCACTGGAACA
  AAGAAAATCTGGCGTTGTGAAAGCAATAGATCTCATACTACAATTGCCAAATACGCACAATACCAGGCTT
  CCTCCTTCCAGGAATCATTGAGAGAAGAAAATGAGAAAAGAACACAACACAAAGATCATTCAGATAACGA
  ATCCACATCTTCAGAGAATTCTGGAAGGAGAAGGAAAGGACCTTTTAAAACCATAAAATTTGGGACCAAC
  ATTGACCTCTCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAACTGACTAAACTTCCTGCTTTTGCGC
  GTGTGGTGTCAGCAGGAAATCTTCTAACCCATGTTGGGCATACCATTCTGGGCATGAATACAGTACAACT
  GTATATGAAAGTTCCAGGGAGTCGGACACCAGGTCACCAAGAAAATAACAACTTCTGCTCTGTTAACATA
  AATATTGGTCCAGGAGATTGTGAATGGTTTGTTGTACCTGAAGATTATTGGGGTGTTCTGAATGACTTCT
  GTGAAAAAAATAATTTGAATTTTTTAATGAGTTCTTGGTGGCCCAACCTTGAAGATCTTTATGAAGCAAA
  TGTCCCTGTGTATAGATTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTGCATTGG
  GTTCAAGCTGTTGGCTGGTGCAATAACATTGCCTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAAT
  TGGCAGTGGAACGGTATGAATGGAACAAATTGAAAAGTGTGAAGTCACCAGTACCCATGGTGCATCTTTC
  CTGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTTTG
  AAAATTCTGAAGCAATATCAGACATTGAGAGAAGCTCTTGTTGCAGCAGGAAAAGAGGTTATATGGCATG
  GGCGGACAAATGATGAACCAGCTCATTACTGTAGCATTTGTGAGGTGGAGGTTTTTAATCTGCTTTTTGT
  CACTAATGAAAGCAATACTCAAAAAACCTACATAGTACATTGCCATGATTGTGCACGAAAAACAAGCAAA
  AGTTTGGAAAATTTTGTGGTGCTCGAACAGTACAAAATGGAGGACCTAATCCAAGTTTATGATCAATTTA
  CACTAGCTCTTTCATTATCATCCTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235280 representing NM_001258251
  Red=Cloning site Green=Tags(s)
  
  MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL
  GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY
  NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH
  LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS
  GQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAA
  AWMDLGTLYESCNQPQDAIKCYLNAARSKRCSNTSTLAARIKFLQNGSDNWNGGQSLSHHPVQQVYSLCL
  TPQKLQHLEQLRANRDNLNPAQKHQLEQLESQFVLMQQMRHKEVAQVRTTGIHNGAITDSSLPTNSVSNR
  QPHGALTRVSSVSQPGVRPACVEKLLSSGAFSAGCIPCGTSKILGSTDTILLGSNCIAGSESNGNVPYLQ
  QNTHTLPHNHTDLNSSTEEPWRKQLSNSAQAYLGHLTGFIQDNCNKGLHKSQSSCLSGPNEEQPLFSTGS
  AQYHQATSTGIKKANEHLTLPSNSVPQGDADSHLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTG
  DTSNGCADVKGLSNHVHQLIADAVSSPNHGDSPNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAV
  HTKTDHSVASSPSSAISTATPSPKSTEQRSINSVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRS
  TSQILPSMSVSICPSSTEVLKACRNPGKNGLSNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLEN
  KRDAFFPPLHQFCTNPKNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGT
  KKIWRCESNRSHTTIAKYAQYQASSFQESLREENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTN
  IDLSDNKKWKLQLHELTKLPAFARVVSAGNLLTHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNI
  NIGPGDCEWFVVPEDYWGVLNDFCEKNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHW
  VQAVGWCNNIAWNVGPLTACQYKLAVERYEWNKLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLL
  KILKQYQTLREALVAAGKEVIWHGRTNDEPAHYCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSK
  SLENFVVLEQYKMEDLIQVYDQFTLALSLSSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258251
• ORF Size: 4089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258251.1, NP_001245180.1
• RefSeq Size: 6574 bp
• RefSeq ORF: 4092 bp
• Locus ID: 7404
• Cytogenetics: Yq11.221
• Protein Families: Transmembrane
• MW: 151.8 kDa
• Gene Summary: 'This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]'