UTY (NM_001258249) Human Tagged ORF Clone

CAT#: RC235303

UTY (Myc-DDK tagged) - Homo sapiens ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (UTY), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UTY
• Synonyms: KDM6AL; KDM6C; UTY1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235303 representing NM_001258249
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG
  AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG
  TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA
  GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT
  GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA
  CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
  AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA
  AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT
  TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT
  TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC
  TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG
  AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
  GGCCAATCGTGGTATTTTCTTGGAAGGTGTTATTCAAGTATTGGGAAAGTTCAGGATGCCTTTATATCTT
  ACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGTTGTATCAGCA
  GCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACAATTGGACCATGGGCATGCCGCA
  GCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAAGATGCCATTAAATGCTACCTAA
  ATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAAGAATTAAATTTCTACAGGCTCA
  GTTGTGTAACCTTCCACAAAGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAGGAGGCATGG
  AGCCTACCAATCCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCACAGCAGGCTTATAGAG
  CTCATGATCCAAATACTGAACATGTATTAAACCACAGTCAAACACCAATTTTACAGCAATCCTTGTCACT
  ACACATGATTACTTCTAGCCAAGTAGAAGGCCTGTCCAGTCCTGCCAAGAAGAAAAGAACATCTAGTCCA
  ACAAAGAATGGTTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACAGCAAGTTTATT
  CGTTGTGTTTGACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGATAATTTAAATCC
  AGCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGAGACACAAAGAA
  GTTGCTCAGGTACGAACTACTGGAATTCATAACGGGGCCATAACTGATTCATCACTGCCTACAAACTCTG
  TCTCTAATCGACAACCACATGGTGCTCTGACCAGAGTATCTAGCGTCTCTCAGCCTGGAGTTCGCCCTGC
  TTGTGTTGAAAAACTTTTGTCCAGTGGAGCTTTTTCTGCAGGCTGTATTCCTTGTGGCACATCAAAAATT
  CTAGGAAGTACAGACACTATCTTGCTAGGCAGTAATTGTATAGCAGGAAGTGAAAGTAATGGAAATGTGC
  CTTACCTGCAGCAAAATACACACACTCTACCTCATAATCATACAGACCTGAACAGCAGCACAGAAGAGCC
  ATGGAGAAAACAGCTATCTAACTCCGCTCAGGGGCTTCATAAAAGTCAGAGTTCATGTTTGTCAGGACCT
  AATGAAGAACAACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGCAACTAGCACTGGTATTAAGA
  AGGCGAATGAACATCTCACTCTGCCTAGTAATTCAGTACCACAGGGGGATGCTGACAGTCACCTCTCCTG
  TCATACTGCTACCTCAGGTGGACAACAAGGCATTATGTTTACCAAAGAGAGCAAGCCTTCAAAAAATAGA
  TCCTTGGTGCCTGAAACAAGCAGGCATACTGGAGACACATCTAATGGCTGTGCTGATGTCAAGGGACTTT
  CTAATCATGTTCATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCATGGAGATTCACCAAATTTATT
  AATTGCAGACAATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATGGCAATGTGGGTACTGGAACC
  TGTGACAAAGTGAATAATATTCACCCAGCTGTTCATACAAAGACTGATCATTCTGTTGCCTCTTCACCCT
  CTTCAGCCATTTCCACAGCAACACCTTCTCCTAAATCCACTGAGCAGAGAAGCATAAACAGTGTTACCAG
  CCTTAACAGTCCTCACAGTGGATTACACACAGTCAATGGAGAGGGGCTGGGGAAGTCACAGAGCTCTACA
  AAAGTAGACCTGCCTTTAGCTAGCCACAGATCTACTTCTCAGATCTTACCATCAATGTCAGTGTCTATAT
  GCCCCAGTTCAACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAATGGCTTGTCTAATAGCTGCAT
  TTTGTTAGATAAATGTCCACCTCCAAGACCACCAACTTCACCATACCCACCCTTGCCAAAGGACAAGTTG
  AATCCACCCACACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACATCAATTTT
  GTACAAATCCAAAAAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGATCTTGGACT
  TTTCTCTACCAAAACTTTGGTAGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGTTGCTGCAA
  CCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGCGTTGTGAAAGCAATAGATCTCATA
  CTACAATTGCCAAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCATTGAGAGAAGAAAATGAGAA
  AAGAACACAACACAAAGATCATTCAGATAACGAATCCACATCTTCAGAGAATTCTGGAAGGAGAAGGAAA
  GGACCTTTTAAAACCATAAAATTTGGGACCAACATTGACCTCTCTGATAACAAAAAGTGGAAGTTGCAGT
  TACATGAACTGACTAAACTTCCTGCTTTTGCGCGTGTGGTGTCAGCAGGAAATCTTCTAACCCATGTTGG
  GCATACCATTCTGGGCATGAATACAGTACAACTGTATATGAAAGTTCCAGGGAGTCGGACACCAGGTCAC
  CAAGAAAATAACAACTTCTGCTCTGTTAACATAAATATTGGTCCAGGAGATTGTGAATGGTTTGTTGTAC
  CTGAAGATTATTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTTTTAATGAGTTCTTG
  GTGGCCCAACCTTGAAGATCTTTATGAAGCAAATGTCCCTGTGTATAGATTTATTCAGCGACCTGGAGAT
  TTGGTCTGGATAAATGCAGGCACTGTGCATTGGGTTCAAGCTGTTGGCTGGTGCAATAACATTGCCTGGA
  ATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTATGAATGGAACAAATTGAAAAG
  TGTGAAGTCACCAGTACCCATGGTGCATCTTTCCTGGAATATGGCACGAAATATCAAAGTCTCAGATCCA
  AAGCTTTTTGAAATGATTAAGTATTGTCTTTTGAAAATTCTGAAGCAATATCAGACATTGAGAGAAGCTC
  TTGTTGCAGCAGGAAAAGAGGTTATATGGCATGGGCGGACAAATGATGAACCAGCTCATTACTGTAGCAT
  TTGTGAGGTGGAGGTTTTTAATCTGCTTTTTGTCACTAATGAAAGCAATACTCAAAAAACCTACATAGTA
  CATTGCCATGATTGTGCACGAAAAACAAGCAAAAGTTTGGAAAATTTTGTGGTGCTCGAACAGTACAAAA
  TGGAGGACCTAATCCAAGTTTATGATCAATTTACACTAGCTCTTTCATTATCATCCTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235303 representing NM_001258249
  Red=Cloning site Green=Tags(s)
  
  MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL
  GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY
  NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH
  LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS
  GQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAA
  AWMDLGTLYESCNQPQDAIKCYLNAARSKRCSNTSTLAARIKFLQAQLCNLPQSSLQNKTKLLPSIEEAW
  SLPIPAELTSRQGAMNTAQQAYRAHDPNTEHVLNHSQTPILQQSLSLHMITSSQVEGLSSPAKKKRTSSP
  TKNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRANRDNLNPAQKHQLEQLESQFVLMQQMRHKE
  VAQVRTTGIHNGAITDSSLPTNSVSNRQPHGALTRVSSVSQPGVRPACVEKLLSSGAFSAGCIPCGTSKI
  LGSTDTILLGSNCIAGSESNGNVPYLQQNTHTLPHNHTDLNSSTEEPWRKQLSNSAQGLHKSQSSCLSGP
  NEEQPLFSTGSAQYHQATSTGIKKANEHLTLPSNSVPQGDADSHLSCHTATSGGQQGIMFTKESKPSKNR
  SLVPETSRHTGDTSNGCADVKGLSNHVHQLIADAVSSPNHGDSPNLLIADNPQLSALLIGKANGNVGTGT
  CDKVNNIHPAVHTKTDHSVASSPSSAISTATPSPKSTEQRSINSVTSLNSPHSGLHTVNGEGLGKSQSST
  KVDLPLASHRSTSQILPSMSVSICPSSTEVLKACRNPGKNGLSNSCILLDKCPPPRPPTSPYPPLPKDKL
  NPPTPSIYLENKRDAFFPPLHQFCTNPKNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQ
  PADENWDPTGTKKIWRCESNRSHTTIAKYAQYQASSFQESLREENEKRTQHKDHSDNESTSSENSGRRRK
  GPFKTIKFGTNIDLSDNKKWKLQLHELTKLPAFARVVSAGNLLTHVGHTILGMNTVQLYMKVPGSRTPGH
  QENNNFCSVNINIGPGDCEWFVVPEDYWGVLNDFCEKNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGD
  LVWINAGTVHWVQAVGWCNNIAWNVGPLTACQYKLAVERYEWNKLKSVKSPVPMVHLSWNMARNIKVSDP
  KLFEMIKYCLLKILKQYQTLREALVAAGKEVIWHGRTNDEPAHYCSICEVEVFNLLFVTNESNTQKTYIV
  HCHDCARKTSKSLENFVVLEQYKMEDLIQVYDQFTLALSLSSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258249
• ORF Size: 4332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258249.1, NP_001245178.1
• RefSeq Size: 6817 bp
• RefSeq ORF: 4335 bp
• Locus ID: 7404
• Cytogenetics: Yq11.221
• Protein Families: Transmembrane
• MW: 160.6 kDa
• Gene Summary: 'This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]'