BOULE (BOLL) (NM_001284362) Human Tagged ORF Clone

CAT#: RC237222

BOLL (myc-DDK-tagged) - Human boule-like RNA-binding protein (BOLL), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BOLL
• Synonyms: BOULE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237222 representing NM_001284362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAACATCAAACCAGATGCAAACAGATTCATTATCTCCATCCCCTAATCCTGTGTCACCTGTGCCTT
  TGAATAACCCAACAAGTGCCCCAAGATATGGAACAGTGATCCCTAATCGCATCTTTGTAGGAGGAATTGA
  TTTTAAGACAAACGAAAGTGATTTAAGAAAATTTTTTTCCCAGTATGGGTCTGTGAAAGAAGTGAAGATT
  GTAAATGACAGAGCTGGAGTATCCAAAGGGTATGGTTTCGTCACTTTTGAAACACAAGAAGATGCACAAA
  AAATTTTACAAGAGGCTGAAAAACTTAATTATAAGGATAAGAAGCTGAACATTGGTCCAGCAATAAGAAA
  ACAACAAGTAGGGATCCCTCGTTCTAGTATAATGCCAGCAGCTGGAACAATGTATCTAACAACTTCAACT
  GGATATCCTTATACTTACCATAATGGTGTTGCTTATTTTCATACTCCAGAGGTAACTTCGGTCCCACCGC
  CTTGGCCTTCACGTTCTGTATGTAGCTCCCCTGTGATGGTAGCTCAGCCCATTTATCAGCAACCTGCATA
  TCACTACCAGGCCACCACACAGTATTTACCAGGACAGTGGCAGTGGAGTGTTCCTCAGCCTTCTGCCTCT
  TCTGCTCCATTCTTATACCTGCAACCTTCTGAGGTTATTTATCAACCAGTGGAAATTGCACAGGATGGTG
  GATGTGTTCCTCCTCCACTGTCTCTGATGGAAACTTCAGTTCCAGAGCCTTATTCTGATCATGGAGTTCA
  AGCAACATATCACCAGGTTTATGCTCCAAGTGCCATCACTATGCCTGCGCCTGTGATGCAGCCTGAGCCA
  ATTAAAACAGTGTGGAGCATTCATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237222 representing NM_001284362
  Red=Cloning site Green=Tags(s)
  
  MQTSNQMQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKI
  VNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTST
  GYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSAS
  SAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEP
  IKTVWSIHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284362
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001284362.1, NP_001271291.1
• RefSeq Size: 2925
• RefSeq ORF: 870
• Locus ID: 66037
• MW: 32.4 kDa
• Gene Summary: This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]